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| Hagel,Jillian M.; Krizevski,Raz; Kilpatrick,Korey; Sitrit,Yaron; Marsolais,Frédéric; Lewinsohn,Efraim; Facchini,Peter J.. |
| Khat (Catha edulis Forsk.) is a flowering perennial shrub cultivated for its neurostimulant properties resulting mainly from the occurrence of (S)-cathinone in young leaves. The biosynthesis of (S)-cathinone and the related phenylpropylamino alkaloids (1S,2S)-cathine and (1R,2S)-norephedrine is not well characterized in plants. We prepared a cDNA library from young khat leaves and sequenced 4,896 random clones, generating an expressed sequence tag (EST) library of 3,293 unigenes. Putative functions were assigned to > 98% of the ESTs, providing a key resource for gene discovery. Candidates potentially involved at various stages of phenylpropylamino alkaloid biosynthesis from L-phenylalanine to (1S,2S)-cathine were identified. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Khat; (S)-cathinone; Phenylpropylamino alkaloids biosynthesis; EST library; Gene discovery. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400017 |
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