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	Provedor de dados BABT (1) BJPS (1) Autor Chelladurai,Gladis Raja Malar (1) Chinnachamy,Chellaram (1) Enez,Baris (1) Fincan,Sema Aguloglu (1) Karakaya,Adem (1) Ozdemir,Sadin (1) Mais... Palavra-chave Α-Amylase (2) Antidiabetic (1) Apple Juice Clarification (1) Detergents (1) Raw starch (1) Salacia oblonga (1) Terpenoids (1) Thin-Layer Chromatography (TLC) (1) Α-Glucosidase (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2018 (2) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última A Novel Raw Starch Hydrolyzing Thermostable α-Amylase Produced by Newly Isolated Bacillus mojavensis SO-10: Purification, Characterization and Usage in Starch Industries BABT Ozdemir,Sadin; Fincan,Sema Aguloglu; Karakaya,Adem; Enez,Baris. ABSTRACT The aim of this study is the production, purification, and characterisation of thermostable raw starch hydrolyzing α-amylase produced by Bacillus mojavensis SO-10. The maximum production conditions of α-amylase were found at 36th hour, 35 °C and pH 7.0. We utilized three steps to purify the thermostable α-amylase and as a result, 34-fold and 18% yield were obtained. The molecular weight of purified α-amylase was determined as 73 kD. The Km and Vmax rates were detected as 0.010 mM and 3.38 µmol min−1, respectively. This purified α-amylase exhibited the highest activity at pH 5.0-6.0 and 70 ºC and showed stability over a wide variety of pH and temperature at 4.0-8.0, and 40-50 ºC, respectively. The thermostable purified α-amylase exhibited stability... Tipo: Info:eu-repo/semantics/article Palavras-chave: Α-Amylase; Raw starch; Thin-Layer Chromatography (TLC); Detergents; Apple Juice Clarification. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100412 Alpha amylase and Alpha glucosidase inhibitory effects of aqueous stem extract of Salacia oblonga and its GC-MS analysis BJPS Chelladurai,Gladis Raja Malar; Chinnachamy,Chellaram. ABSTRACT Our present investigation deals with the phytochemical screening, estimation of total flavonoids, terpenoids and tannin contents to evaluate the anti-diabetic activities of Salacia oblonga stem followed by GC-MS analysis. It explores the natural compounds and the potential α-amylase and α-glucosidase inhibitory actions of stem extracts. The aqueous stem extract was selected from other extracts (ethanol, acetone, petroleum ether and chloroform) for the in vitro study of anti-diabetic activity by alpha amylase and alpha glucosidase inhibitory assays. The stem extract was also analyzed by gas chromatography mass spectrometry to identify the natural chemical components. Phytochemical analysis of aqueous stem extract showed major classes of secondary... Tipo: Info:eu-repo/semantics/article Palavras-chave: Salacia oblonga; Antidiabetic; Α-Amylase; Α-Glucosidase; Terpenoids. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100606 Registros recuperados: 2 Primeira ... 1 ... Última

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