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Wollheim,Claudia; Guerra,Ivani Maria F; Conte,Vania D; Hoffman,Sheila P; Schreiner,Fernando J; Delamare,Ana Paula L; Barth,Afonso L; Echeverrigaray,Sérgio; Costa,Sérgio Olavo P da. |
OBJECTIVES: To determine the prevalence of class A extended spectrum β-lactamases (ESBL)-producing Escherichia coli and Klebsiella spp., and to investigate clonality among ESBL-producing isolates of nosocomial and community infections. METHODS: The study involved 354 nosocomial infections samples and 992 community infections samples, obtained between 2003 and 2006 at Caxias do Sul, RS. The detection of ESBL was performed by the disk-diffusion test. Presence of blaCTX-M, blaSHV and blaTEM β-lactamase genes was evaluated by PCR, and genomic typing was determined by pulsed-field gel electrophoresis analysis. RESULTS: Higher frequency of ESBL-producing isolates were detected among nosocomial samples of E. coli (6.7%) and Klebsiella (43.7%), than those obtained... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Β-lactamases; Polymerase chain reaction; Bacterial typing techniques. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200008 |
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Santos,Daniella Fabíola dos; Pimenta,Fabiana Cristina; Alves,Rodrigo; Montalvão,Edlaine Rodrigues; Santos,Daniela Braz dos; Carmo Filho,José Rodrigues do. |
This study was developed to evaluate the prevalence of extended-spectrum β-lactamases (ESBL) producing Klebsiella pneumoniae in two hospitals (A and B) in Goiânia, GO, Brazil. The antimicrobial susceptibility of the isolates was determined using the MicroScan WalkAway (Dade Behring, USA). Tests to evaluate the genetic correlation between the isolates were also performed. For the ESBL phenotypic test, the Double-disk diffusion (DD) method was used. The strainsisolated in Hospital B were submitted to DNA analysis by pulsed-field gel electrophoresis (PFGE). The study showed high prevalence of ESBL-producing K. pneumoniae (25% in hospital A and 66.7% in hospital B), with high rates of antimicrobial resistance. The most active compound was imipenem (100%... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Extended-spectrum β-lactamases (ESBL); Klebsiella pneumoniae; Β-lactamases; Bacterial resistance. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400002 |
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