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| BULAT,TANJA; KETA,OTILIJA; KORIĆANAC,LELA; ŽAKULA,JELENA; PETROVIĆ,IVAN; RISTIĆ-FIRA,ALEKSANDRA; TODOROVIĆ,DANIJELA. |
| ABSTRACT Ionizing radiation induces DNA double strand breaks (DSBs) that trigger phosphorylation of the histone protein H2AX (γH2AX). Immunofluorescent staining visualizes formation of γH2AX foci, allowing their quantification. This method, as opposed to Western blot assay and Flow cytometry, provides more accurate analysis, by showing exact position and intensity of fluorescent signal in each single cell. In practice there are problems in quantification of γH2AX. This paper is based on two issues: the determination of which technique should be applied concerning the radiation dose, and how to analyze fluorescent microscopy images obtained by different microscopes. HTB140 melanoma cells were exposed to γ-rays, in the dose range from 1 to 16 Gy. Radiation... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ApoTome software; AxioImagerA1 microscope; Immunofluorescence microscopy; Western blot; ΓH2AX. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100127 |
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| Xu,Xianrong; Lu,Jing; Sheng,Hongqiang; Zhang,Long; Gan,Tieer; Zhang,Jianyun; Xu,Yuying; Zhu,Xinqiang; Yang,Jun. |
| The melamine and cyanuric acid (CA) complex has been suggested to cause the toxic effects observed in melamine-contaminated food or milk. However, the cytotoxic and genotoxic effects of co-exposure to melamine and CA are not fully clear. Therefore, the cytotoxic effects of melamine and CA were first examined by co‐exposure in human kidney 293 cells using the MTT assay. During a 24-h period for the three concentrations tested (0.5, 1, and 5 mg/mL), neither melamine nor CA alone showed significant toxic effects on 293 cells at 0.5 mg/mL, while higher concentrations led to decreased in cell viability. However, co-exposure to several combinations of melamine and CA [100:1, 10:1, 1:10, and 1:100 (v:v), at a final concentration of 0.5 mg/mL] did cause... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Melamine; Cyanuric acid; ΓH2AX; DNA damage; Genotoxicity. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500612 |
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