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การผลิตโพลีโคลนอลแอนติซีรัมที่จำเพาะต่อ Streptomyces-PR15 และการใช้ตรวจติดตามเชื้อรอบผิวรากต้นกล้าพริก Thai Agricultural
Weerakorn Saengsai; Petcharat Thummabenjapone.
The antagonistic Streptomyces-PR15 is a broad spectrum against various plant pathogenic fungi and bacteria of economic crops. To develop the ELISA technique for detection the Streptomyces-PR15 in bioproducts and plant surrounding environment, the production of polyclonal antiserum against Streptomyces-PR15 was carried out. The antigen prepared from Streptomyces-PR15 whole cell proteins was mixed with Freund’s incomplete adjuvant (1:1, v/v) was injected to in New Zealand White rabbit with immunization program as follows, intramuscular injection for 3 times then only antigen was intravenous injected for 2 times. Bleeding was done at 10 days after final immunization and then at weekly interval for 8 weeks. The antigen was re-intravenous injected at 14 days...
Tipo: PhysicalObject Palavras-chave: Pepper; Chilli seedlings; Antagonist; Streptomyces-PR15; Polyclonal antiserum; Bioproduct; Elisa; Antiserum against; พริกหนุ่มเขียว; พริกขี้หนู; รากต้นกล้า; จุลินทรีย์ปฏิปักษ์; เชื้อแบคทีเรีย สเตรปโตมัยซีส; โพลีโคลนอลแอนติบอดี; การตรวจติดตามเชื้อ; ชีวภัณฑ์; คุณภาพแอนติซีรัม; แอนติบอดีจำเพาะ.
Ano: 2013 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5804
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