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Effect of 0.9% saline solution and phosphate buffer saline at different temperatures and incubation times on the morphology of goat preantral follicles Braz. J. Vet. Res. Anim. Sci.
Santos,Regiane Rodrigues dos; Silva,José Roberto Viana; Costa,Sonia Helena Furtado; Rodrigues,Ana Paula Ribeiro; Lôbo,Raimundo Nonato Braga; Figueiredo,José Ricardo de.
The present work investigated the efficiency of 0.9% saline solution and Phosphate Buffered Saline (PBS) in the preservation of goat preantral follicles in situ at different temperatures and incubation times. The ovarian pair of each animal was divided into 19 fragments. One ovarian fragment was taken randomly and fixed (control). The other 18 fragments were randomly distributed in tubes containing 0.9% saline solution or PBS at 4, 20 or 39 ºC for 4, 12 or 24 h. A total of 5,921 preantral follicles were examined. The quality of preantral follicles was evaluated by classical histology. The storage of ovarian fragments in 0.9% saline solution or PBS at 4 ºC did not reduce significantly the percentage of morphologically normal follicles when compared with the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Goat; Preantral follicle; Preservation; 0.9% saline solution; PBS.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000500007
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