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| Costa,Eula Maria de M. B; Pimenta,Fabiana Cristina; Luz,Christian; Oliveira,Valéria de; Oliveira,Marília; Bueno,Elda; Petrofeza,Silvana. |
| Beauveria bassiana genetic diversity and ability to synthesize quercetin 2,3-dioxygenase (quercetinase) were analyzed. B. bassiana isolates, obtained from Brazilian soil samples, produced quercetinase after induction using 0.5 g/L quercetin. B. bassiana ATCC 7159 (29.6 nmol/mL/min) and isolate IP 11 (27.5 nmol/ml/min) showed the best performances and IP 3a (9.5 nmol/mL/min) presented the lowest level of quercetinase activity in the culture supernatant. A high level of polymorphism was detected by random amplified polymorphic DNA (RAPD) analysis. The use of internal-transcribed-spacer ribosomal region restriction fragment length polymorphism (ITS-RFLP) did not reveal characteristic markers to differentiate isolates. However, the ITS1-5.8S-ITS2 region... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Beauveria bassiana quercetinase quercetin-2; 3-dioxygenase genetic diversity. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100002 |
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