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Changes in diapause related gene expression pattern during early embryonic development in HCl-treated eggs of bivoltine silkworm Bombyx mori (Lepidoptera: Bombycidae) BABT
Sasibhushan,Sirigineedi; Ponnuvel,Kangayam M.; Vijayaprakash,Nanjappa B..
Investigation of differential expression of diapause related genes (five metabolic, five heat shock protein and one translational regulatory) in HCl-treated (non-diapause) and untreated (diapause) eggs of B. mori during early embryogenesis (up to 48h following oviposition) revealed the up-regulation of sorbitol dehydrogenase upon HCl treatment, indicating increased glycogen synthesis for further embryonic development but, down-regulation of phosphofructo kinase gene expression after 18h of oviposition indicating an arrest of glycerol and sorbitol conversion. The expression of poly A binding protein gene expression was higher upon HCl treatment, revealing the initiation of translation. The expression levels of other genes analyzed did not vary...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hibernation; Acid treatment; Metabolic genes; Heat shock protein genes.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100001
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Acetic acid as an intervention strategy to decontaminate beef carcasses in mexican commercial slaughterhouse Ciênc. Tecnol. Aliment.
Reyes Carranza,Laura; Rubio Lozano,Maria Salud; Méndez Medina,Ruben Danilo; Rodarte,Maria Del Carmen Wacher; Núñez Espinosa,Jose Fernando; Velázquez Camacho,Bertha Lucila; Macedo,Renata Ernlund Freitas.
Beef can be contaminated during the slaughter process, thus other methods, besides the traditional water washing, must be adopted to preserve meat safety. The objective of this study was to evaluate the effect of 2% acetic acid interventions on the reduction of indicator bacteria on beef carcasses at a commercial slaughterhouse in Mexico. Reduction was measured by the count of mesophilic aerobic bacteria (TPC), total coliform (TC), and fecal coliform (FC) (log CFU/ cm²). Among the different interventions tested, treatments combining acetic acid solution sprayed following carcass water washing had greater microbial reduction level. Acetic acid solution sprayed at low pressure and longer time (10-30 psi/ 60 s) reached higher TPC, TC, and FC reductions than...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine carcass; Acid treatment; Microbial load.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000300009
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