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Carvalho,Dayse Cristina de; Silva,André Luís Lopes da; Schuck,Mariane Ruzza; Purcino,Marivel; Tanno,Guilherme Nakao; Biasi,Luiz Antonio. |
The aim of this work was to study the influence of sucrose and glucose, amino acids and BAP (6-Benzylaminopurine) levels on in vitro shoot regeneration of fox grape cv. Bordô and grapevine cv. Chardonnay. The nodal segments from micropropagated material were used as explants and half-strength MS medium as the basal medium. Sucrose and glucose at 15, 30 and 45 g.L-1 were tested as a carbon source and the supplementation of adenine, asparagine, alanine, glycine, cysteine, glutamine, arginine was tested at 40 g.L-1. The BAP levels (1 and 5 μM) in solid and double-phase media were evaluated and compared with a control medium without BAP. Bordô had best in vitro growth than Chardonnay. Sucrose was a better carbohydrate source than glucose for both the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Arginine; Nodal segment; Glucose; Adenine; Glycine. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200004 |
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