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| Registros recuperados: 53 | |
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| Swiech,Kamilla; da Silva,Gracinda M.C; Zangirolami,Teresa C; Iemma,Mônica R.C; Selistre-de-Araújo,Heloísa S; Suazo,Cláudio A.T. |
| We present kinetic and physiological data regarding the culturing of rCHO-K1 cells on various microcarriers, to evaluate the potential of this culture strategy for mass production of these cells and expression of a recombinant disintegrin. Cultures were performed in 500 mL spinner flasks in DMEM culture medium with 10% v/v fetal calf serum, gently shaken at 37ºC, pH 7.4, in a 10% v/v CO2 atmosphere. The following values were obtained, respectively, for the adhesion time-constant Ka (h) and specific growth rate μmax (d-1) on each microcarrier: Cytodex 1 (0.91, 0.45), Cultispher S (0.28, 0.34), Immobasil FS (0.85, 0.52) and Pronectin F (5.12, 0.67). Metabolic characteristics showed some variation among the cultures with the four microcarriers, the... |
| Tipo: Journal article |
Palavras-chave: Adhesion; Animal cell; CHO-K1; Growth; Microcarrier; Recombinant disintegrin. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200004 |
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| Labreuche, Yannick; Soudant, Philippe; Goncalves, Madeleine; Lambert, Christophe; Nicolas, Jean-louis. |
| Vibrio aestuarianus strain 01/32 was previously shown to be pathogenic to Crassostrea gigas juveniles. To investigate virulence mechanisms of this pathogen, we studied the toxicity to oysters of its extracellular products (ECPs). ECPs displayed lethality to animals, with a LD50 value of 3.3 mu g/g body weight. To determine the oyster cellular immune responses induced by these ECPs, we further examined in vitro their effects on C. gigas hemocytes, using flow cytometric-based hemocyte assays. Treatment of hemolymph with ECPs caused a significant inhibition of hemocyte phagocytosis and adhesive capabilities. In contrast, the pathway of reactive oxygen species production was enhanced by higher ECP concentrations. Exposure of hemocytes to live bacteria induced... |
| Tipo: Text |
Palavras-chave: Vibrio aestuarianus; ECPs; Crassostrea gigas; ROS production; Adhesion; Phagocytosis; Flow cytometry; Bivalve immunity. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1035.pdf |
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| Cognard, Jean Yves; Davies, Peter; Gineste, B; Sohier, L. |
| This study is concerned with improving performance of composite structures through the use of adhesive bonding, particularly for marine and underwater applications. Some preliminary results from tests on simple composite/composite assemblies are given first. Difficulty in modelling the failure of even these simple joints highlighted the need for more reliable constituent input data. The first objective was to define an experimental methodology enabling the adhesives of interest to be characterised up to failure. A metal metal assembly was considered, in order to concentrate on the analysis of the behaviour of thin adhesive films. The aims were to characterise the adhesive, analyse its non-linear behaviour, and study the influence of parameters such as film... |
| Tipo: Text |
Palavras-chave: Finite element analysis; Adhesion. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-765.pdf |
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| Lambert, Christophe; Soudant, Phillippe; Degremont, Lionel; Delaporte, Maryse; Moal, Jeanne; Boudry, Pierre; Jean, Frédéric; Huvet, Arnaud; Samain, Jean-francois. |
| High variability among individuals is often encountered when hemocyte characteristics are measured in bivalves. Such variability is suspected to result partly from genetic factors. In this study, hemocyte characteristics of six families of Crassostrea gigas were compared by flow cytometry at one sampling date in October 2001. These families were obtained from a nested, half-sibling cross design, and reared from July to October 2001 at three sites distributed along the French Atlantic coast from north to south: Baie des Veys (Normandy), Riviere d'Auray (Brittany) and Ronce (Marennes-oleron Basin, Poitou Charentes). Among the 15 measured hemocyte characteristics, production of reactive oxygen species (ROS) of untreated hemocytes (maintained in filtered... |
| Tipo: Text |
Palavras-chave: Genetic; Flow cytometry; Reactive oxygen species ROS; Adhesion; Hemocyte; Crassostrea gigas; Summer mortality. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3054.pdf |
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| Nassif, L. Abi; Rioual, S.; Trepos, Rozenn; Fauchon, M.; Farah, W.; Hellio, C.; Abboud, M.; Lescop, B.. |
| Microorganisms have the ability to settle on nearly all man-made surfaces in contact with seawater and subsequently to form biofilm. Biofilms control and removal is necessary in the sectors of maritime transport, energy... In this work, we present the development of new pure calcium, zinc or copper alginate, but also mixed Ca/Cu and Ca/Zn alginate hydrogels. These materials have been evaluated for their potential inhibition of adhesion of two key biofilm-forming microalgae Halamphora coffeaeformis and Cylindrotheca closterium). All the tested materials have presented high adhesion inhibition about 80%). Copper-base materials present a high toxicity against H. coffeaeformis. Pure zinc alginate is also toxic for this strain. However, the addition of calcium... |
| Tipo: Text |
Palavras-chave: Biofilm; Adhesion; Alginate; Zinc; Biomaterials; Microalgae. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00605/71671/70168.pdf |
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| Leroy, C.; Delbarre Ladrat, Christine; Ghillebaert, F.; Compere, Chantal; Combes, D.. |
| The antifouling potential of commercial hydrolases, four proteases, seven glycosidases and one lipase was evaluated on the adhesion of marine Pseudoalteromonas sp. D41. The experimental method, adapted to screen antifouling agents, was based on bacterial adhesion in natural sterile sea water in a microtiter plate and on total biomass quantification by the fluorescent dye DAPI (4[prime]6-diamidino-2-phenylindole). Savinase (subtilisin) was the most effective hydrolase in both the prevention of bacterial adhesion and the removal of adhered bacteria. However, some enzymatic preparations tested such as Amano protease were not only ineffective but also increased the number of adhered bacterial cells. Enumeration using epifluorescence microscopy of CTC... |
| Tipo: Text |
Palavras-chave: Marine biofilm; Antifouling; Pseudoalteromonas; Enzymes; Adhesion. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-6140.pdf |
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| Darold, L; Blanco, E. |
| One of the major defects that can be found on composite sandwich structures is poor bonding of the core on the exterior skins, particularly with the technique of bonding under vacuum. Therefore, we have looked at all the possible means of detecting these bonding defects, with two objectives: to be able to control integrally a piece of large dimensions, and to be able to control the piece in the course of manufacturing, that is to say after the bonding on the first skin. All of the non-destructive test methods now known have therefore been tested, and in particularly infrared thermography, ultrasonic control, and acoustic methods. This study, carried out in collaboration with the CETIM, the STCAN, Aerospatiale and other laboratories and companies without... |
| Tipo: Text |
Palavras-chave: Adhesion; Ship technology; Composite materials; Contrôle non destructif; Structure sandwich. |
| Ano: 1992 |
URL: http://archimer.ifremer.fr/doc/1992/acte-1049.pdf |
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| Cognard, Jean Yves; Davies, Peter; Sohier, S; Creac' Hcadec, R. |
| The objective of this study is to define a reliable tool for dimensioning of adhesively bonded assemblies, particularly for marine and underwater applications. This paper presents experimental and numerical results, which describe the non-linear behaviour of an adhesive in a bonded assembly for various loadings. A modified Arcan fixture, well-suited for the study of the behaviour of bonded metal-metal assemblies, was developed in order to focus on the analysis of the behaviour of the adhesive in thin films. The edge effects are minimised by using an appropriate design of the substrates so that experimental results give reliable data. To analyze the kinematics of the deformation of the adhesive joint non-contact extensometry techniques were employed. Thus,... |
| Tipo: Text |
Palavras-chave: Non linear behaviour; Finite element; Arcan fixture; Marine structures; Composite; Adhesion. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2079.pdf |
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| Fera, P; Prieur, D. |
| Before the building of an (OTEC) power plant, and experimental station was built near the entrance of the Bay of Brest in order to do preliminary studies. Using these facilities, the bacterial colonization of three substrata (stainless steel UB6, aluminium AG4, polycarbonate filters) was studied in an open seawater system, with a speed of 0,7 m s super(-1). The study was performed between May 1983 and May 1984, including seven experiments of 15 days each. Bacteria colonizing the metallic surfaces were counted by scanning electron microscopy, and bacteria colonizing the polycarbonate filters counted by epifluorescence microscopy. On the three substrata, bacterial colonization occurred in three successive stages: initial colonization, sometimes unsteady... |
| Tipo: Text |
Palavras-chave: Fouling; OTEC plants; Bacteria; Colonization; Adhesion; Microbiological analysis; Surface films. |
| Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/1984/acte-964.pdf |
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| Cognard, J. Y.; Sohier, L.; Davies, Peter. |
| Composite materials are a key element in weight reduction strategies, so the analysis of the mechanical behavior of assemblies of composite modules is of great importance. Failure initiation in bonded assemblies involving composites is often associated with crack initiation in the adhesive or delamination of the composite plies close to the adhesive joint, caused by interlaminar or through-thickness stresses. Thus, the analysis of the behavior of composites and their assemblies under out-of-plane loadings is necessary in order to optimize such structures. However, few experimental devices are proposed in the literature and they often require thick composite specimens which are not representative of most industrial applications. This paper describes the use... |
| Tipo: Text |
Palavras-chave: Laminates; Adhesion; Stress concentrations; Mechanical testing. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00026/13740/11039.pdf |
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| Chiapparrone,María L; Soto,Pedro; Catena,María. |
| Campylobacter fetus is extracellular bacteria of the genital tract of cattle. They cause infertility and abortion, but there is no documented information on the susceptibility of bovine sperm cells to this bacteria. The aim of this present work was to study the effects provoked by Campylobacter fetus subsp. venerealis when in interaction with bovine sperm cells. The bovine spermatozoa were obtained frozen bovine semen pooled from uninfected bulls, and were exposed to living campylobacter over different periods of time. Light microscopy and scanning electron microscopy first revealed a tropism, then a close proximity followed by tight adhesion between these two different cells. A decrease in the spermatozoa motility was observed. Motile bacteria were... |
| Tipo: Journal article |
Palavras-chave: Adhesion; Campylobacter fetus subsp. venerealis; Sperm cells. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400040 |
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| Registros recuperados: 53 | |
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