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| FELMER,RICARDO N; CLARK,JOHN A. |
| The feasibility of ablating differentiated adipocytes and the mechanism of cell ablation with a suitable prodrug activating system is described. The system is based on the use of E. coli nitroreductase (NTR) enzyme that activates certain nitro compounds, such as the antitumor drug CB1954, into cytotoxic DNA interstrand cross-linking agents. Differentiated preadipocyte cells (3T3L1) transfected with an aP2 driven nitroreductase construct were efficiently killed after incubation with medium containing the prodrug CB1954, while untransfected cells were not affected. It was demonstrated that the mechanism of cell ablation is apoptosis and that the system has a bystander effect mediated by a toxic metabolite of the prodrug. The described system should provide a... |
| Tipo: Journal article |
Palavras-chave: Genetic ablation; NTR/CB1954; Adipocytes; Differentiation. |
| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300009 |
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| Gomes,Júlia Ariana de Souza; Faria,Bruno Generoso; Silva,Viviam de Oliveira; Zangerônimo,Márcio Gilberto; Miranda,José Rafael; Lima,Adriene Ribeiro de; Pereira,Rosemary Gualberto Fonseca Alvarenga; Pereira,Luciano José. |
| The objective of this study was to evaluate the influence of integral and decaffeinated coffee brews (Coffea arabica L and C. canephora Pierre) on the metabolic parameters of rats fed with hyperlipidemic diet. Thirty male Wistar rats (initial weight of 270 g ± 20 g) were used in the study, which were divided into six groups five each. The treatments were normal diet, hyperlipidemic diet, hyperlipidemic diet associated with integral coffee arabica or canephora brews (7.2 mL/kg/day) and hyperlipidemic diet associated to decaffeinated arabica, or canephora brews, using the same dosage. After 41 days, performance analyses were conducted.The rats were then euthanized and the carcasses were used for the analysis of dried ether extract and crude protein.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lipids; Adipocytes; Hyperlipidemia; Caffeine; Decaffeination. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500015 |
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| Ultrastructural phenotypic transitional features were noted between adult adipocytes and fibroblasts in the subcutaneous adipose tissue of the dorsal pad of normal adult Wistar rats of both sexes, weighing 180-260 g, after acute injury either by the implantation of small (1.8 x 1 x 0.4 cm) perforated plastic boxes or by local heat application. Soon after the inflicted damage, fat-containing cells presented variable shapes. Early after damage, some of these cells were round, adipocyte-like, with numerous and large cytoplasmic fat droplets. A few days later, fat-containing cells became elongated, with the fat droplets in their cytoplasm becoming smaller and less numerous. The cells also showed a prominent active rough endoplasmic reticulum and newly formed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Adipocytes; Adipocyte potential for differentiation; Adipose tissue damage. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500009 |
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| Ceddia,R.B.; William Jr.,W.N.; Lima,F.B.; Carpinelli,A.R.; Curi,R.. |
| The OB protein, also known as leptin, is secreted by adipose tissue, circulates in the blood, probably bound to a family of binding proteins, and acts on central neural networks regulating ingestive behavior and energy balance. The two forms of leptin receptors (long and short forms) have been identified in various peripheral tissues, a fact that makes them possible target sites for a direct action of leptin. It has been shown that the OB protein interferes with insulin secretion from pancreatic islets, reduces insulin-stimulated glucose transport in adipocytes, and increases glucose transport, glycogen synthesis and fatty acid oxidation in skeletal muscle. Under normoglycemic and normoinsulinemic conditions, leptin seems to shift the flux of metabolites... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptin; Insulin secretion; Muscle metabolism; Insulin resistance; Adipocytes. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600001 |
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| Covas,D.T.; Siufi,J.L.C.; Silva,A.R.L.; Orellana,M.D.. |
| Bone marrow contains a population of stem cells that can support hematopoiesis and can differentiate into different cell lines including adipocytes, osteocytes, chondrocytes, myocytes, astrocytes, and tenocytes. These cells have been denoted mesenchymal stem cells. In the present study we isolated a cell population derived from the endothelium and subendothelium of the umbilical cord vein which possesses morphological, immunophenotypical and cell differentiation characteristics similar to those of mesenchymal stem cells isolated from bone marrow. The cells were isolated from three umbilical cords after treatment of the umbilical vein lumen with collagenase. The cell population isolated consisted of adherent cells with fibroblastoid morphology which, when... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Mesenchymal stem cells; Umbilical vein; Adipocytes; Osteocytes; Cell differentiation. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900006 |
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| Sertié,R.A.L.; Andreotti,S.; Proença,A.R.G.; Campaña,A.B.; Lima,F.B.. |
| As it is a common observation that obesity tends to occur after discontinuation of exercise, we investigated how white adipocytes isolated from the periepididymal fat of animals with interrupted physical training transport and oxidize glucose, and whether these adaptations support the weight regain seen after 4 weeks of physical detraining. Male Wistar rats (45 days old, weighing 200 g) were divided into two groups (n=10): group D (detrained), trained for 8 weeks and detrained for 4 weeks; and group S (sedentary). The physical exercise was carried out on a treadmill for 60 min/day, 5 days/week for 8 weeks, at 50-60% of the maximum running capacity. After the training protocol, adipocytes isolated from the periepididymal adipose tissue were submitted to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Physical detraining; Adipocytes; Glucose uptake; Glucose oxidation; Lipogenesis. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700650 |
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| Caminhotto,R de O.; Sertié,R.A.L.; Andreotti,S.; Campaãa,A.B.; Lima,F.B.. |
| Due to the presence of the renin-angiotensin system (RAS) in tissues and its specific influence on white adipose tissue, fat cells are possible targets of pharmacological RAS blockers commonly used as anti-hypertensive drugs. In the present study, we investigated the effects of different RAS blockers on fat cell metabolism, more specifically on lipolysis, lipogenesis and oxidation of energy substrates. Isolated primary adipocytes were incubated with different RAS blockers (aliskiren, captopril and losartan) in vitro for 24 h and lipolysis, lipogenesis and glucose oxidation capacities were determined in dose-response assays to a β-adrenergic agonist and to insulin. Although no change was found in lipolytic capacity, the RAS blockers modulated lipogenesis... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Adipocytes; Lipolysis; Lipogenesis; Glucose; Renin-angiotensin system. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800608 |
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| 何, 茂龍; 盧, 尚建; 岡, 英之; 日高, 智; 松長, 延吉; 左, 久; He, Mao Long; Roh, Sang-Gun; Oka, Hideyuki; Hidaka, Satoshi; Matsunaga, Nobuyoshi; Hidari, Hisashi. |
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Palavras-chave: Fatty acid; Adipocytes; Steer; Cell diameter. |
| Ano: 1997 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2622 |
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