Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: 

RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 27
Primeira ... 12 ... Última
Imagem não selecionada

Imprime registro no formato completo
Association of the severity of tongue lesions in broiler chickens with the quality of corn in feed PAB
Vaccari,Isabel Cristina Mores; Duarte,Vinicius; Rodrigues,Denise Russi; Santos,Fabiana Ramos dos; Minafra,Cibele Silva.
Abstract: The objective of this work was to determine the correlation of the physical attributes of corn and the mycotoxins present in its grains with the severity of tongue lesions in broiler breeder roosters. The occurrence of the aflatoxin, vomitoxin, zearalenone, T-2 toxin, and fumonisin mycotoxins was detected in the corn grains used in the feed formulation for breeder chickens. The percentages of moisture and broken grains were positively correlated (Pearson’s correlation) with the presence of aflatoxin. Milder lesions (grade 1) are positively correlated with aflatoxin and negatively correlated with fumonisin, and the most severe lesions (grade 3) are positively correlated with vomitoxin, fumonisin, and T-2 toxin.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Aviculture; Fumonisin; Mycotoxin; T-2 toxin; Vomitoxin..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017001000942
Imagem não selecionada

Imprime registro no formato completo
Aflatoxin Redux: Does European Aflatoxin Regulation Hurt Groundnut Exporters from Africa? AgEcon
Xiong, Bo; Beghin, John C..
Replaced with revised version of paper 07/02/10.
Tipo: Conference Paper or Presentation Palavras-chave: Food safety; Standards; Aflatoxin; Maximum Residues Limit; Groundnut; Gravity equation; Africa; Market access; EU; Agricultural and Food Policy; Community/Rural/Urban Development; Crop Production/Industries; Food Consumption/Nutrition/Food Safety; Food Security and Poverty; International Development; International Relations/Trade; Q17; F13.
Ano: 2010 URL: http://purl.umn.edu/61314
Imagem não selecionada

Imprime registro no formato completo
What You Don’t Know Can Hurt You: Micronutrient Content and Fungal Contamination of Foods in Developing Countries AgEcon
Hoffmann, Vivian.
The negative effects of micronutrient deficiencies on human capital acquisition and economic productivity are well documented. A less well understood but potentially serious threat to human health in developing countries is the contamination of food crops by fungal toxins. This paper surveys what is known about the health and economic burdens attributable to insufficient micronutrients and toxic contamination of food in developing countries, discusses consumer demand for micronutrients and food safety, and describes some of the challenges to improving population nutrition, particularly in rural areas.
Tipo: Journal Article Palavras-chave: Food safety; Micronutrients; Aflatoxin; Developing countries; Food Consumption/Nutrition/Food Safety; International Development.
Ano: 2009 URL: http://purl.umn.edu/55548
Imagem não selecionada

Imprime registro no formato completo
The Preventive Effects of Different Doses of Glucomannan on Experimental Aflatoxicosis in Japanese Quails Rev. Bras. Ciênc. Avic.
Yavuz,O; Özdemir,Ö; Ortatatli,M; Atalay,B; Hatipoglu,F; Terzi,F.
ABSTRACT This experimental study was performed to investigate whether there is a protective effect of different doses of Glucomannan using against aflatoxicosis in Japanese quail, and pathological changes and relative organ weights were compared. In the experiment, 60 one-day old male Japanese quails were used as divided into six different groups. Experimental groups were designated as Control(C), aflatoxin(A), glucomannan(GM), 2-fold dose of glucomannan(2GM), aflatoxin+glucomannan(A+GM) and aflatoxin+2-fold dose of glucomannan(A+2GM). While control group quails fed the standard ration as ad libitum, other groups were fed with the administrations additionally to standard diet respectively; 2mg/kg of aflatoxin to group A, 1g/kg of glucomannan to group GM,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Glucomannan; Histopathology; Quail.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000300409
Imagem não selecionada

Imprime registro no formato completo
Evaluation of yeast cell wall on the performance of broiles fed diets with or without mycotoxins Rev. Bras. Ciênc. Avic.
Santin,E; Paulillo,AC; Nakagui,LSO; Alessi,AC; Maiorka,A.
This experiment aimed at evaluating the effects of the interactions between aflatoxin (500 or 250 ppb) and ochratoxin (500 or 250 ppb), and the possible benefits of adding yeast cell wall to prevent the effects of these mycotoxins in broiler chickens. Relative organ weight gain and live performance were evaluated at 21 and 42 days of age. Results indicated that at the levels of mycotoxins included in the experimental diets, ochratoxin reduced feed intake and body weight gain, and aflatoxin only affect feed intake of 21-day-old birds. No interaction was observed between aflatoxin and ochratoxin at the levels used in experimental study. Yeast cell wall did not significantly reduced the deleterious effects of ochratoxins. No significant differences were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Ochratoxin; Yeast cell wall; Broilers; Performance.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000400004
Imagem não selecionada

Imprime registro no formato completo
Performance and egg quality of laying hens fed diets containing aflatoxin, fumonisin and adsorbent Rev. Bras. Ciênc. Avic.
Siloto,EV; Sartori,DRS; Oliveira,EFA; Sartori,JR; Fascina,VB; Berto,DA.
The effects of aflatoxin and fumonisin and their combination on egg production and quality, as well as the efficacy of a mycotoxin adsorbent in reducing or eliminating these effects in commercial layers. A number of 168 layers with initial age of 37 weeks were submitted to an experimental period of 56 days. A completely randomized experimental design in a 3x2+1 factorial arrangement was applied (3 treatments with mycotoxins: aflatoxin (AF), fumonisin (FU), or aflatoxin + fumonisin (AF+FU); 2 treatments with or without adsorbent; and a control group that was fed no mycotoxins, nor adsorbent), totaling 7 treatments with 6 replicated of 4 birds/cage. The dietary inclusion levels were 1ppm AF, 25ppm FU, and 2 kg adsorbent/ton feed. Birds fed AF presented the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adsorbent; Aflatoxin; Fumonisin; Layers; Performance.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2011000100004
Imagem não selecionada

Imprime registro no formato completo
Effects of Yeast Glucomannan and Sodium Bentonite on the Toxicity of Aflatoxin in Broilers Rev. Bras. Ciênc. Avic.
Azizpour,A; Moghadam,N.
ABSTRACT The aim of this study was to evaluate the efficacy of a yeast glucomannan (YG) and sodium bentonite (SB) in reducing the toxicity of aflatoxin for broilers fed a diet naturally contaminated with aflatoxin. In total, 300 7-day-old Ross 308 broilers were randomly assigned to 10 dietary treatments, consisting of a diet with no aflatoxin (positive control), a diet naturally contaminated with 250 ppb aflatoxin (negative control), and eight aflatoxin-contaminated diets supplemented with different SB and YG and their combinations. Serum biochemical parameters and liver pathological changes were investigated when broilers were 42 days old. The birds fed the negative control diet presented lower uric acid, cholesterol, and triglyceride levels and higher in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Biochemical parameters; Broilers; Pathological changes; Yeast glucomannan.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015001000007
Imagem não selecionada

Imprime registro no formato completo
Mycotoxin contamination of diets for beef cattle finishing in feedlot R. Bras. Zootec.
Custódio,Letícia; Prados,Laura Franco; Yiannikouris,Alexandros; Holder,Vaughn; Pettigrew,James; Kuritza,Leandro; Resende,Flávio Dutra de; Siqueira,Gustavo Rezende.
ABSTRACT The objective of this survey was to identify what mycotoxins were present in ingredients used in diets offered to beef cattle in feedlots and their concentrations. The survey covered 30 Brazilian feedlots located in the five major beef-producing states. Samples of total mixed ration (TMR) and ingredients were collected and analyzed for mycotoxin using ultra-performance liquid chromatography coupled to tandem mass spectrometry. Mycotoxin concentrations were further interpreted according to known species-specific sensitivities and normalized according to the principles of toxic equivalent factors (risk equivalent quantities - REQ) expressed in µg kg−1 of aflatoxin B1-equivalent. Forty percent of the visited feedlots had apparent fungi in TMR....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Beef cattle; Feedlots; Fumonisin; Ingredients.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100724
Imagem não selecionada

Imprime registro no formato completo
Aflatoxin removal of peanut meals with aqueous ethanol Scientia Agricola
Fonseca,H.; Regitano-d'Arce,M.A.B..
A preliminary approach to achieve compatible simultaneous extraction of aflatoxin and residual oil from pressed oil meals was conducted to determine the minimum amount of water to be added to ethanol versus necessary time to achieve complete removal of aflatoxin. Commercial anhydrous, 96, 93 and 90°GL ethanol were utilized in trials with Soxhlet extractors. Commercial anhydrous ethanol did not remove aflatoxin completely and the extraction efficiency in minutes was directly proportional to the amount of water present in the ethanol, as expected. Although 96°GL ethanol was efficient, alcoholic strengths between 96 and 99°GL need be evaluated to improve oil extraction, provided they do not decrease aflatoxin removal efficiency.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Peanut meal; Oil meals; Removal; Extraction; Aqueous ethanol; Ethanol.
Ano: 1993 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161993000100021
Imagem não selecionada

Imprime registro no formato completo
การติดเชื้อรา Aspergillus flavus และการปนเปื้อนสารอะฟลาทอกซินในถั่วลิสง 6 พันธุ์เมื่อกระทบแล้งในช่วงปลายของการเจริญเติบโต Thai Agricultural
Ratanaporn Koolachart; Teerayoot Girdthai; Sanun Jogloy; Nimitr Vorasoot; Sopone Wongkaew; Aran Patanothai.
End of season drought at pod filling and maturity stages can severely reduce yield and increase the risk of aflatoxin contamination. Peanut varieties with resistance to drought may reduce Aspergillus flavus infection and aflatoxin contamination. The objectives of this study were to investigate the effects of late season drought during 80 days after planting (DAP) until harvest on A. flavus infection and aflatoxin contamination and to estimate the correlation coefficients between traits related to aflatoxin contamination and the correlation coefficients between traits related to drought resistance (SPAD chlorophyll meter reading (SCMR) and specific leaf area (SLA)) and traits to aflatoxin contamination (A. flavus infection and aflatoxin contamination) of 6...
Tipo: Collection Palavras-chave: Arachis hypogaea L.; Aflatoxin; Drought tolerance; Peanut; Aspergillus flavus; ถั่วลิสง; อะฟลาทอกซิน; การปนเปื้อนเชื้อรา; ผลกระทบแล้ง; การทนแล้ง; ระยะสร้างฝัก; ผลผลิต; พันธุ์.
Ano: 2011 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5109
Imagem não selecionada

Imprime registro no formato completo
ผลประโยชน์ด้านสุขภาพอนามัยของผู้บริโภคในการประกาศมาตรฐานการปนเปื้อนสารอะฟลาทอกซินในถั่วลิสง Thai Agricultural
Thanaporn Athipanyakul; Sanan Jogloy; Weera Pakuthai.
Objective of this study was to assess health economic benefits of published standard of aflatoxin residue in peanut by measuring the cost of illness. Data were carried out from the total number of 99 producers and 100 consumers in the Northeast and the North of Thailand. The results showed that 3.8 persons in 100,000 persons would be at the risk of liver cancer due to consumption of contaminated peanut. The benefit gain of 1,793 to 2,658 million baht per year was estimated in case of the enforcement of new standard. However, other benefits of all stakeholders along supply chain of peanut must be considered for implementation of the policy. The fair comparison of benefits and costs of policy implementation of aflatoxin residue standard must also be taken...
Tipo: Collection Palavras-chave: Peanut; Aflatoxin; Economic benefits; Cost of illness; ถั่วลิสง; อะฟลาทอกซิน; ผลประโยชน์ทางเศรษฐศาสตร์; ต้นทุนความเจ็บป่วย; การปนเปื้อนเชื้อรา.
Ano: 2011 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5106
Imagem não selecionada

Imprime registro no formato completo
ผลการเสริมไฮเดรตโซเดียมแคลเซียมอลูมิโนซิลิเกต (Detox®) ในอาหารที่ปนเปื้อนอะฟลาทอกซินต่อลักษณะทางพยาธิสภาพและสมรรถนะ การเจริญเติบโตของเป็ดเนื้อ Thai Agricultural
Thianchai Sroirak; Sawitree Wongtangtintharn; Komkrich Pimpakdee; Jaowamarn Khajarern.
The aim of this study was to evaluate the efficacy of hydrate sodium calcium aluminosilicate (HSCAS; Detox®) in binding aflatoxin (AF) and ameliorating their mycotoxicity in meat type duck diets. Measurement the growth performance and pathology by HSCAS were supplemented to 60 or 120 ppb AF contaminated diets at either 0.10 or 0.20%. Body weight gain were reduced by 21.45% and mortality was increased by 7.50% in duck fed diet contaminated with AF at 120 ppb compared to duck fed control diet (30 ppb AF) for the 4 wk experimental period. However, dietary HSCAS supplementation effectively alleviated overall toxicity induced by AF. The significant treatment-related changes in feather growth, eye necrosis, web-toe hemorrhage, leg deformity, tibia bone porosity,...
Tipo: PhysicalObject Palavras-chave: Meat-type ducks; Aflatoxin; Adsorbent; Growth performance; เป็ดเนื้อ; อะฟลาทอกซิน; สารดูดซับ; สมรรถนะการเจริญเติบโต; ไฮเดรตโซเดียมแคลเซียมอลูมิโนซิลิเกต; พยาธิสภาพ.
Ano: 2013 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5409
Imagem não selecionada

Imprime registro no formato completo
อะฟลาทอกซินในถั่วลิสง: ข้อเสนอวิธีแก้ปัญหา Thai Agricultural
Sopone wongkaew; Sanun Jogloy.
Contamination of peanuts by aflatoxins, the carcinogenic mycotoxins produced by Aspergillus flavus and A. parasiticus, has been a chronic problem in Thailand. This article presented the general knowledge of aflatoxins, their harms, factors affecting the fungal infection and aflatoxin contamination in peanuts and proposed a solution of the problem throughout the peanut production chain and its consumption. The solution should consist of encouraging growers to follow the Good Agricultural Practices (GAP) in peanut production, enforcing the Good Manufacturing Practices (GMP) standard on peanut shellers, enforcing the regulation and limits of aflatoxin contamination of raw shelled peanuts to ensure their quality and safety in both domestic and imported...
Tipo: Collection Palavras-chave: Peanuts; Aflatoxin; Good agricultural practices; GAP; ถั่วลิสง; สารก่อมะเร็ง; การปฏิบัติทางการเกษตรที่ดี; อะฟลาทอกซิน; การปนเปื้อน; สารก่อมะเร็ง.
Ano: 2011 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5193
Imagem não selecionada

Imprime registro no formato completo
Minimizing of Aflatoxin in Grain in the Tropics Thai Agricultural
Siriacha, P..
Palavras-chave: Aflatoxin.
Ano: 1999 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5033
Imagem não selecionada

Imprime registro no formato completo
Occurrence of mycootoxins in alfalfa (Medicago sativa L.), sorghum [Sorghum bicolor (L.) Moench], and grass (Cenchrus ciliaris L.) retailed in the state of Nuevo León, México Agrociencia
Huerta-Treviño,Alejandra; Dávila-Aviña,Jorge E.; Sánchez,Eduardo; Heredia,Norma; García,Santos.
Abstract Mycotoxins are secondary metabolites produced by specific filamentous fungi that are common contaminants of agricultural commodities. These compounds are toxic to humans and animals, and they are a health problem worldwide. Mycotoxin-containing feeds can cause serious diseases in farm animals and substantial economic losses. Therefore, the objective of this study was to determine the occurrence and levels of six mycotoxins in three types of forage at retail in the state of Nuevo León, México. The hypothesis was that all types of forages were contaminated with at least one of the six mycotoxins analyzed. One hundred and twenty samples of alfalfa (Medicago sativa L.), sorghum [Sorghum bicolor (L.) Moench], and grass (Cenchrus ciliaris L.) used for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycotoxins; Forages; Aflatoxin; Fumonisin; Ochratoxin.
Ano: 2016 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952016000700825
Imagem não selecionada

Imprime registro no formato completo
Nutritional changes in powdered red pepper upon in vitro infection of Aspergillus flavus BJM
Tripathi,Smita; Mishra,H.N..
Quantitative losses in various biochemical constituents like capsaicin, carotenes, ascorbic acid, polyphenols, mineral matter, sugars (soluble and insoluble), protein and fat were estimated after the successful growth of Aspergillus flavus for 30 days on powdered red pepper. The fungal biomass was measured by ergosterol content and Aflatoxin B1 by HPLC. Amongst the various nutritional constituents evaluated for nutritional losses and changes the highest nutritional loss was reported in total carotenoids (88.55%) followed by total sugars (85.5%). The protein content of the infected sample increased from 18.01% to 23%. The nutritional profile of chilli powder (Capsicum annum var. sannam L.) shows highest share of total soluble sugars (32.89%) and fiber...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus flavus; Chili powder; Nutritional changes; Aflatoxin.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100024
Imagem não selecionada

Imprime registro no formato completo
Molecular characterisation of Aspergillus flavus isolates from peanut fields in India using AFLP BJM
Singh,Diwakar; Radhakrishnan,T.; Kumar,Vinod; Bagwan,N.B.; Basu,M.S.; Dobaria,J.R.; Mishra,Gyan P.; Chanda,S.V..
Aflatoxin contamination of peanut, due to infection by Aspergillus flavus, is a major problem of rain-fed agriculture in India. In the present study, molecular characterisation of 187 Aspergillus flavus isolates, which were sampled from the peanut fields of Gujarat state in India, was performed using AFLP markers. On a pooled cluster analysis, the markers could successfully discriminate among the ‘A’, ‘B’ and ‘G’ group A. flavus isolates. PCoA analysis also showed equivalent results to the cluster analysis. Most of the isolates from one district could be clustered together, which indicated genetic similarity among the isolates. Further, a lot of genetic variability was observed within a district and within a group. The results of AMOVA test revealed that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; AMOVA; ELISA; Genetic diversity; Groundnut; PCA.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300673
Imagem não selecionada

Imprime registro no formato completo
Production of aflatoxins by Aspergillus flavus and of fumonisins by Fusarium species isolated from Brazilian sorghum BJM
Silva,Josefa B. da; Dilkin,Paulo; Fonseca,Homero; Corrêa,Benedito.
Fifty-nine Aspergillus flavus and 35 Fusarium verticillioides strains, isolated from freshly harvested (10) and stored (130) Brazilian sorghum samples, were tested regarding their ability to produce aflatoxins (coconut milk agar) and fumonisins (rice culture), respectively. Aflatoxins B1 and B2 were detected by TLC, and fumonisins B1 and B2 were analyzed by HPLC. Thirty-eight (64.4%) A. flavus strains produced detectable levels of aflatoxins at concentrations ranging from 12.00 to 3282.50 µg/kg (AFB1 + AFB2), while thirty two (91%) F. verticillioides strains produced FB1 at concentrations ranging from 0.12 to 5.38 µg/g. Two F. proliferatum strains produced low fumonisin levels. The toxigenic potential of A. flavus (64.4%) and F. verticillioides (91.5%)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxigenicity; Aspergillus flavus; Fusarium verticillioides; Mycotoxins; Sorghum; Aflatoxin; Fumonisin.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200002
Imagem não selecionada

Imprime registro no formato completo
The potential effects of Zataria multiflora Boiss essential oil on growth, aflatoxin production and transcription of aflatoxin biosynthesis pathway genes of toxigenic Aspergillus parasiticus BJM
Yahyaraeyat,R.; Khosravi,A.R.; Shahbazzadeh,D.; Khalaj,V..
This study aims at evaluating the effects of Zataria multiflora (Z. multiflora) essential oil (EO) on growth, aflatoxin production and transcription of aflatoxin biosynthesis pathway genes. Total RNAs of Aspergillus parasiticus (A.parasiticus) ATCC56775 grown in yeast extract sucrose (YES) broth medium treated with Z. multiflora EO were subjected to reverse transcription-polymerase chain reaction (RT-PCR). Specific primers of nor-1, ver-1, omt-A and aflR genes were used. In parallel mycelial dry weight of samples were measured and all the media were assayed by high-pressure liquid chromatography (HPLC) for aflatoxinB1 (AFB1), aflatoxinB2 (AFB2), aflatoxinG1 (AFG1), aflatoxinG2 (AFG2) and aflatoxin total (AFTotal) production. The results showed that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zataria multiflora; Reverse transcription-polymerase chain reaction; Aflatoxin; Aspergillus parasiticus.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200045
Imagem não selecionada

Imprime registro no formato completo
The application of PCR in the detection of mycotoxigenic fungi in foods BJM
Konietzny,Ursula; Greiner,Ralf.
It is estimated that 25 to 50% of the crops harvested worldwide are contaminated with mycotoxins. Because of the toxic and carcinogenic potential of mycotoxins, there is an urgent need to develop detection methods that are rapid and highly specific. The highly advanced physico-chemical methods for the analysis of mycotoxins in use, have the disadvantage that highly sophisticated clean-up and/or derivatization procedures must be applied. An alternative could be the detection of the mycotoxigenic moulds themselves, especially as molecular techniques have been introduced recently as powerful tools for detecting and identifying fungi. PCR methods for the detection of aflatoxigenic Aspergilli, patulin-producing Penicillum and trichothecene- as well as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Fumonisin; Patulin; Polymerase chain reaction; Trichothecene.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400001
Registros recuperados: 27
Primeira ... 12 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional