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| Santos,Anderson F.; Pires,Fabiano; Jesus,Hugo E.; Santos,André L.S.; Peixoto,Raquel; Rosado,Alexandre S.; D'Avila-Levy,Claudia M.; Branquinha,Marta H.. |
| Two psychrophilic bacterial samples were isolated from King George Island soil, in Antarctica. The phylogenetic analysis based on the 16S rRNA (rrs) gene led to the correlation with the closest related isolates as Sporosarcina aquimarina (99%) and Algoriphagus antarcticus(99%), with query coverage of 99% and 98%, respectively.The spent culture media from both isolates displayed proteolytic activities detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis containing gelatin as protein substrate. Under the employed conditions, S. aquimarina showed a 55 kDa protease with the best activity detected at pH 7.0 and at 27°C. A. antarcticusalso showed a single extracellular protease, however its molecular mass was around 90kDa and its best activity... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Algoriphagus antarcticus; Antarctica; Sporosarcina aquimarina; Protease. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100109 |
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