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| Registros recuperados: 28 | |
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| Lima,Domênica Teixeira de; Piedras,Fernanda Reinhardt; Nogueira,Flávia Previero; Richard,Eduardo da Cunha; Tocci,Bianca Reis Castaldi; Alba,Jose Juan Barrera; Moser,Gleyci Aparecida Oliveira. |
| ABSTRACT To describe potential intraspecific variation in phosphorus incorporation in two strains of Phaeodactylum tricornutum (Bohlin), Ub3 and Ub7, alkaline phosphatase (AP) activity was evaluated via enzyme-labeled fluorescence assay. Analysis using the probe ELF-97(r) provides individual evaluation, and therefore can determine the nutritional status of inorganic phosphorus in phytoplanktonic cells. Bioassays compared the control treatment to both phosphate-enriched and phosphate-depleted treatments by varying only the phosphate concentration in the media. The P. tricornutum strains exhibited differences in their development when incubated in the phosphate-enriched media. The development of the Ub7 strain differed by exhibiting "luxury uptake" and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alkaline phosphatase; Auxotrophy; Marine diatom; Organic phosphorus incorporation; Phosphate limitation. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062016000300445 |
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| Alfonso-Torres,K.A.; Gargaglioni,L.H.; Pizauro,J.M.; Faria Filho,D.E.; Furlan,R.L.; Macari,M.. |
| The effect of breeder age on long bone development was studied in chicken embryos from 12 days of incubation until hatching. Fertile eggs were incubated and randomly assigned in a 2 x 6 factorial arrangement (two breeder ages - 38 and 60 weeks and six incubation days - 12, 14, 16, 18, 20, and 21). Enzymatic activity of acid and alkaline phosphatases in tibial epiphyses and weights as well as length and width in tibias and femurs of the embryos were determined. Tartrate-resistant acid and alkaline phosphatases activity in epiphyses was not affected by breeder age. Absolute weight and width of femur and tibia were larger in 60-week-old embryos compared to 38-week-old. Enzymatic activity and morphometric measurements increased with incubation day,... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Chicken embryo; Alkaline phosphatase; Epiphyses; Growth plate; Long bones. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100031 |
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| Sousa,C.; Abreu,H.; Viegas,C.; Azevedo,J.; Reis,R.; Gomes,M.; Dias,I.. |
| O objetivo deste trabalho foi estudar o padrão de variação da atividade sérica da fosfatase alcalina total (tALP), da isoenzima óssea da fosfatase alcalina (BALP) e da fosfatase ácida resistente ao tartarato (TRAP), assim como a variação da concentração dos minerais séricos durante o processo de cicatrização de fraturas ósseas no cão. A variação sérica destes marcadores do metabolismo ósseo foi avaliada em nove cães com fraturas diafisárias fechadas de ossos longos, submetidas a tratamento cirúrgico para osteosíntese. Durante o período pós-operatório, sete animais evoluíram no sentido de uma normal união óssea, sendo que dois deles desenvolveram um processo de não união óssea. Foram observados, relativamente à BALP, valores de actividade sérica mais... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dog; Alkaline phosphatase; Tartrate-resistant acid phosphatase; Fracture healing process. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000400029 |
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| Mamun,Md. Abdullah Al; Hosen,Mohammad Jakir; Islam,Kamrul; Khatun,Amina; Masihul Alam,M.; Al-Bari,Md. Abdul Alim. |
| BACKGROUND: Tridaxprocumbens flavonoids (TPFs) are well known for their medicinal properties among local natives. Besides traditionally used for dropsy, anemia, arthritis, gout, asthma, ulcer, piles, and urinary problems, it is also used in treating gastric problems, body pain, and rheumatic pains of joints. TPFs have been reported to increase osteogenic functioning in mesenchymal stem cells. Our previous study showed that TPFs were significantly suppressed the RANKL-induced differentiation of osteoclasts and bone resorption. However, the effects of TPFs to promote osteoblasts differentiation and bone formation remain unclear. TPFs were isolated from Tridax procumbens and investigated for their effects on osteoblasts differentiation and bone formation by... |
| Tipo: Journal article |
Palavras-chave: Alkaline phosphatase; Bone formation; Osteoblast differentiation; Osteocalcin. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100065 |
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| Fernandes,Mileni da Silva; Iano,Flávia Godoy; Rocia,Vivian; Yanai,Marcela Mitsuko; Leite,Aline de Lima; Furlani,Tatiana Almeida; Buzalaf,Marília Afonso Rabelo; Oliveira,Rodrigo Cardoso de. |
| The aim of this study was to compare the effect of fluoride (F) on alkaline phosphatase activity in the liver and plasma of the rats. Four groups of male Wistar rats (n=6), which received drinking water containing 5, 15 or 50 ppm F or deionized water (control) throughout the experiment were included in the study. The animals were euthanized and had their tissues and blood plasma collected for the analysis of fluoride and alkaline phosphatase. There was an increase in F concentration in most tissues in the animals treated with higher F concentrations, except for the heart. The alkaline phosphatase assay showed an increase in the activity in the liver and blood plasma of the animals treated with fluoride concentrations of 15 and 50 ppm (p<0.05). This... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alkaline phosphatase; Fluoride; Liver; Kidney. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600014 |
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| Morales,A.C.; Nozawa,S.R.; Thedei Jr.,G.; Maccheroni Jr.,W.; Rossi,A.. |
| A constitutive alkaline phosphatase was purified to apparent homogeneity as determined by polyacrylamide gel electrophoresis from mycelia of the wild strain 74A of the mold Neurospora crassa, after growth on acetate and in the presence of saturating amounts of inorganic phosphate (Pi) for 72 h at 30ºC. The molecular mass was 58 kDa and 56 kDa as determined by exclusion chromatography and SDS-PAGE, respectively. This monomeric enzyme shows an apparent optimum pH ranging from 9.5 to 10.5 and Michaelis kinetics for the hydrolysis of p-nitrophenyl phosphate (the Km and Hill coefficient values were 0.35 mM and 1.01, respectively), alpha-naphthyl phosphate (the Km and Hill coefficient values were 0.44 mM and 0.97, respectively), ß-glycerol phosphate (the Km and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neurospora crassa; Fungi; Alkaline phosphatase; L-histidinol-Pi phosphatase. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800006 |
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| Munekata,R.V.; Terreri,M.T.R.A.; Peracchi,O.A.B.; Len,C.; Lazaretti-Castro,M.; Sarni,R.O.S.; Hilário,M.O.E.. |
| Our objective was to evaluate the concentrations of serum 25-hydroxyvitamin D [25(OH)D], serum calcium, serum phosphorus, alkaline phosphatase, and parathormone (PTH) in patients with polyarticular juvenile idiopathic arthritis (JIA) and to associate them with disease duration and activity, bone mineral density and use of medications. In a cross-sectional and controlled study, 30 patients with polyarticular JIA were evaluated and compared to 30 healthy individuals matched for age and gender. Clinical status, anthropometry, laboratory markers in both patients and controls, and bone mineral density, only in the patients, were measured. Of the 30 patients included in the study, 23 (76.7%) were female and 16 (53.3%) non-Caucasian; mean age was 14 years (range... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Juvenile idiopathic arthritis; Serum vitamin D deficiency; Calcium; Alkaline phosphatase. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100098 |
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| Mota,A.; Silva,P.; Neves,D.; Lemos,C.; Calhau,C.; Torres,D.; Martel,F.; Fraga,H.; Ribeiro,L.; Alçada,M.N.M.P.; Pinho,M.J.; Negrão,M.R.; Pedrosa,R.; Guerreiro,S.; Guimarães,J.T.; Azevedo,I.; Martins,M.J.. |
| Alkaline phosphatase (ALP) is important in calcification and its expression seems to be associated with the inflammatory process. We investigated the in vitro acute effects of compounds used for the prevention or treatment of cardiovascular diseases on total ALP activity from male Wistar rat heart homogenate. ALP activity was determined by quantifying, at 410 nm, the p-nitrophenol released from p-nitrophenylphosphate (substrate in Tris buffer, pH 10.4). Using specific inhibitors of ALP activity and the reverse transcription-polymerase chain reaction, we showed that the rat heart had high ALP activity (31.73 ± 3.43 nmol p-nitrophenol·mg protein-1·min-1): mainly tissue-nonspecific ALP but also tissue-specific intestinal ALP type II. Both ALP isoenzymes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Heart; Alkaline phosphatase; Polyphenol-rich beverages; Steroid hormones; Methylxanthines. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700009 |
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| Ferreira-Nozawa,Monica S.; Nozawa,Sérgio R.; Martinez-Rossi,Nilce M.; Rossi,Antonio. |
| In this communication, we show that the growth of isolate H6 of the dermatophyte Trichophyton rubrum on non-buffered medium and under saturating phosphate conditions is dependent on the initial growth pH, with an apparent optimum at pH 4.0. In addition, irrespective of the initial growth pH, the pH of the medium alteredduring cultivation reaching values that ranged from 8.3 to 8.9. Furthermore, this isolate synthesized and secreted almost the same levels of an alkaline phosphatase with an apparent optimum pH ranging from 9.0 to 10.0 when grown on both low- and high-phosphate medium. Also, this alkaline phosphatase is activated by Mg2+ and is EDTA-sensitive. On the other hand, the very low levels of the enzyme retained by the mycelium grown on buffered... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alkaline phosphatase; Ambient pH; Dermatophytes; Enzyme secretion; Trichophyton rubrum; Pi sensing. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200014 |
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| Nozawa,Sérgio R.; Thedei Jr.,Geraldo; Crott,Luciana S.P.; Barbosa,José E.; Rossi,Antonio. |
| In order to investigate further the adaptive response of moulds to ambient pH, we have measured by ELISA the pho-2-encoded Pi-repressible alkaline phosphatase synthesised by Neurospora crassa. We showed that the 74A and pho-2A strains of this mould secrete similar amounts of the pho-2-encoded enzyme irrespective of ambient pH, when both the preg and pgov genes are not functional, i.e., in strains nuc-2+ growing under Pi-starvation. This suggests that pho-2, which is responsive to Pi starvation via the action of genes nuc-2, preg, pgov and nuc-1, is not a gene responsive to ambient pH and that the differential glycosylation observed for the Pi-repressible alkaline phosphatase retained by the mycelium at pH 5.6 or secreted into the growth medium at pH 8.0 is... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alkaline phosphatase; Ambient pH; Enzyme secretion; Neurospora crassa; Pi sensing. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100018 |
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| Zappa, Sebastien; Rolland, Jean-luc; Flament, Didier; Gueguen, Yannick; Boudrant, Joseph; Dietrich, Jacques. |
| This work reports the first isolation and characterization of an alkaline phosphatase (AP) from a hyperthermophilic archaeon. An AP gene from Pyrococcus abyssi, a euryarchaeon isolated from a deep-sea hydrothermal vent, was cloned and the enzyme expressed in Escherichia coli. Analysis of the sequence showed conservation of the active site and structural elements of the E. coli AP. The recombinant AP was purified and characterized. Monomeric and homodimeric active forms were detected, with a monomer molecular mass of 54 kDa. Apparent optimum pH and temperature were estimated at 11.0 and 70 degreesC, respectively. Thus far, P. abyssi AP has been demonstrated to be the most thermostable AP, with half-lives at 100 and 105 degreesC of 18 and 5 h, respectively.... |
| Tipo: Text |
Palavras-chave: Escherichia coli; Characterization; Isolation; Gene; Alkaline phosphatase; Pyrococcus abyssi; Archaeon. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-1268.pdf |
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| Silva, Flavia; Nicoli, Jacques R.; Zambonino, Jose-luis; Le Gall, Marie-madeleine; Kaushik, Sadasivam; Gatesoupe, François-joel. |
| The sustainable composition of diets of high nutritional value is of the utmost importance for intensive aquaculture. Digestion and absorption of nutrients depends on the activity of the digestive enzymes, in particular those located in the brush border membrane of enterocytes, which are responsible for the final stages of breaking down and absorption of nutrients. In the present study, the substitution of fish meal by lupin or rapeseed meal in the diet was evaluated on gilthead sea bream (Sparus aurata) and goldfish (Carassius auratus). The objectives were to compare the activities of intestinal brush border enzymes in both species fed the control and experimental diets. When gilthead sea bream were fed the vegetable diets, significantly lower activities... |
| Tipo: Text |
Palavras-chave: Sparus aurata; Carassius auratus; Fish meal replacement; Brush border membrane; Digestive enzymes; Alkaline phosphatase; Aminopeptidase N; Maltase; Glutamyl transpeptidase. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00009/11975/8679.pdf |
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| Ma, Hongming; Cahu, Chantal; Zambonino, Jose-luis; Yu, Hairui; Duan, Qingyuan; Le Gall, Marie-madeleine; Mai, Kangsen. |
| Large yellow croaker is an important marine fish species for aquaculture in China. Larval rearing of this species is still based on live prey feeding. The aim of this study was to describe the onset and development of the main digestive enzymes during larval development of this species, in order to obtain essential data for the formulation of a compound diet adapted to larvae. Enzymatic assays were conducted from day I post hatching to day 40 in larvae fed the following live prey sequence: rotifers, Artemia nauplii, copepods, and then frozen copepods. Assays were done on whole larvae until day 19, then on dissected larvae and on purified brush border membranes of intestine. Pancreatic enzymes, amylase, and trypsin, showed high activities from early stages,... |
| Tipo: Text |
Palavras-chave: Trypsin; Pseudosciaena crocea; Fish larval development; Amylase; Aminopeptidase N; Alkaline phosphatase. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-444.pdf |
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| Registros recuperados: 28 | |
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