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Purification and characterization of a thermostable alkaline cellulase produced by Bacillus licheniformis 380 isolated from compost Anais da ABC (AABC)
MARCO,ÉVILIN G. DE; HECK,KARINA; MARTOS,EMERSON T.; VAN DER SAND,SUELI T..
ABSTRACT During composting processes, the degradation of organic waste is accomplished and driven by a succession of microbial populations exhibiting a broad range of functional competencies. A total of 183 bacteria, isolated from a composting process, were evaluated for cellulase activity at different temperatures (37, 50, 60, and 70°C) and pH values. Out of the 22 isolates that showed activity, isolate 380 showed the highest cellulase activity. Its ability to produce cellulase was evaluated in culture medium supplemented with carboxymethyl cellulose, microcrystalline cellulose, wheat straw, and rice husk. The culture medium supplemented with carboxymethyl cellulose induced higher enzyme activity after 6 hours of incubation (0.12 UEA mL-1 min-1). For...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkalophilic enzyme; Carboxymethyl celullase; Compost; High temperature.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502359
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