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Disruption of amylase genes by RNA interference affects reproduction in the Pacific oyster Crassostrea gigas ArchiMer
Huvet, Arnaud; Beguel, Jean-philippe; Pereira Cavaleiro, Nathalia; Thomas, Yoann; Quillien, Virgile; Boudry, Pierre; Alunno-bruscia, Marianne; Fabioux, Caroline.
Feeding strategies and digestive capacities can have important implications for variation in energetic pathways associated with ecological and economically important traits, such as growth or reproduction in bivalve species. Here, we investigated the role of amylase in the digestive processes of Crassostrea gigas, using in vivo RNA interference. This approach also allowed us to investigate the relationship between energy intake by feeding and gametogenesis in oysters. Double-stranded (ds)RNA designed to target the two α-amylase genes A and B was injected in vivo into the visceral mass of oysters at two doses. These treatments caused significant reductions in mean mRNA levels of the amylase genes: −50.7% and −59% mRNA A, and −71.9% and −70.6% mRNA B in 15...
Tipo: Text Palavras-chave: Alpha-Amylase; DsRNA; Gametogenesis; Marine bivalve; Dynamic energy budget.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00271/38239/36422.pdf
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Properties of an amylase from thermophilic Bacillus SP. BJM
Carvalho,Raquel Vieira de; Côrrea,Thamy Lívia Ribeiro; Silva,Júlia Caroline Matos da; Mansur,Luciana Ribeiro Coutinho de Oliveira; Martins,Meire Lelis Leal.
alpha-Amylase production by thermophilic Bacillus sp strain SMIA-2 cultivated in liquid cultures containing soluble starch as a carbon source and supplemented with 0.05% whey protein and 0.2% peptone reached a maximum activity at 32 h, with levels of 37 U/mL. Studies on the amylase characterization revealed that the optimum temperature of this enzyme was 90ºC. The enzyme was stable for 1 h at temperatures ranging from 40-50ºC while at 90ºC, 66% of its maximum activity was lost. However, in the presence of 5 mM CaCl2, the enzyme was stable at 90ºC for 30 min and retained about 58% residual activity after 1 h. The optimum pH of the enzyme was found to be 8.5. After incubation of enzyme for 2 h at pH 9.5 and 11.0 was observed a decrease of about 6.3% and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alpha-Amylase; Thermophilic bacterium; Bacillus sp.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100023
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