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Registros recuperados: 38 | |
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Tavares,Claudinéia R.O.; Bendassolli,José A.; Coelho,Fernando; Sant'ana Filho,Carlos R.; Prestes,Clelber V.. |
This work describes a method for 15N-isotope-labeled glycine synthesis, as well as details about a recovery line for nitrogen residues. To that effect, amination of alpha-haloacids was performed, using carboxylic chloroacetic acid and labeled aqueous ammonia (15NH3). Special care was taken to avoid possible 15NH3 losses, since its production cost is high. In that respect, although the purchase cost of the 13N-labeled compound (radioactive) is lower, the stable tracer produced constitutes an important tool for N cycling studies in living organisms, also minimizing labor and environmental hazards, as well as time limitation problems in field studies. The tests were carried out with three replications, and variable 15NH3(aq) volumes in the reaction were used... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Stable isotope; Glycine; Amino acid. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000300005 |
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Gomes-Marcondes,M.C.C.; Ventrucci,G.; Toledo,M.T.; Cury,L.; Cooper,J.C.. |
Cancer cachexia induces host protein wastage but the mechanisms are poorly understood. Branched-chain amino acids play a regulatory role in the modulation of both protein synthesis and degradation in host tissues. Leucine, an important amino acid in skeletal muscle, is higher oxidized in tumor-bearing animals. A leucine-supplemented diet was used to analyze the effects of Walker 256 tumor growth on body composition in young weanling Wistar rats divided into two main dietary groups: normal diet (N, 18% protein) and leucine-rich diet (L, 15% protein plus 3% leucine), which were further subdivided into control (N or L) or tumor-bearing (W or LW) subgroups. After 12 days, the animals were sacrificed and their carcass analyzed. The tumor-bearing groups showed a... |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Leucine; Amino acid; Walker 256 carcinosarcoma; Body composition; Skeletal muscle protein. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100017 |
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Booz,Maristela Raitz; Kerbauy,Gilberto B.; Guerra,Miguel Pedro; Pescador,Rosete. |
The γ-aminobutyric acid (Gaba) is a non-protein amino acid found in prokaryotes and eukaryotes. Its role in plant development has not been fully established. This study reports a quantification of the levels of endogenous Gaba, as well as investigation of its role in different stages of somatic embryogenesis in Acca sellowiana Berg. (Myrtaceae). Zygotic embryos were used as explants and they were inoculated into the culture medium contained different concentrations of Gaba (0,2, 4, 6, 8 and 10 µM). The highest concentrations of endogenous Gaba were detected between the third and nine days after inoculation, reaching the value of 12.77 µmol.g-1FW. High frequency of somatic embryogenesis was observed in response to 10 µM Gaba. This treatment also resulted in... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amino acid; Pineapple guava; Plant growth. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202009000400003 |
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Silva Júnior,Veredino Louzada da; Donzele,Juarez Lopes; Oliveira,Rita Flávia Miranda de; Saraiva,Alysson; Silva,Francisco Carlos de Oliveira; Kill,João Luís. |
The experiment was conducted to evaluate five nutritional plans based on sequences of standardized ileal digestible lysine: 0.90-0.80-0.70, 1.00-0.90-0.80, 1.10-1.00-0.90, 1.20-1.10-1.00, and 1.30-1.20-1.10% fed to gilts from 60 to 99, 129 to 100, and 130 to 148 days of age, respectively. Eighty commercial hybrid gilts, selected for lean gain, with initial weight of 23.46±0.27kg were allotted in a randomized block design, with five treatments, eight replicates, and two pigs per experimental unit. No effect (P>0.05) of the nutritional plans was verified on daily feed intake, daily weight gain and feed conversion. The nutritional plans had no influence (P>0.05) on any of the carcass traits evaluated (carcass yield, meat amount, and meat yield). The... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amino acid; Carcass; Performance; Pigs; Requirement. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000100118 |
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Altamirano,Claudia; Illanes,Andrés; Canessa,Rossana; Becerra,Silvana. |
Recombinant CHO TF70R cells are able to grow and produce t-PA on serum-free medium BIOPRO1 (BioWhitaker Europe, Belgium). The purpose of the present study was to determine the effect of medium supplementation with vitamins, lipids, and specific amino acids on cell growth, t-PA production and biological functionality. Among vitamins, only biotin, folic acid, cobalamine and benzoic acid were required for improving growth and t-PA production. Lipid supplement allowed a significant increase cell concentration and t-PA specific activity and concentration, though its specific production rate decreased slightly. Medium supplementation with proline, serine and asparagine had also positive effects on cell growth. Besides, the addition of asparagine (even in the... |
Tipo: Journal article |
Palavras-chave: Amino acid; Cholesterol; Vitamins. |
Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100009 |
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Kechaou, Emna; Berge, Jean-pascal; Jaouen, Pascal; Ben Amar, Raja. |
Protein hydrolysate was prepared from common cuttlefish Sepia officinalis by Pepsin. Hydrolysis conditions (time, temperature, and enzyme activity) were optimized by response surface methodology (RSM) using a factorial design. The regression coefficient was close to 0.999, observed during both experimental and validation runs, and indicated the validity of prediction model. An enzyme activity to substrate ratio (X1), 0.04 AU/g protein; time (X2), 85 minutes; and temperature (X3), 45 °C were found to be the optimum conditions for a higher degree of hydrolysis (21%) and nitrogen recovery (90%) using Pepsin. According to amino acid analysis results and chemical score, methionine and histidine are the limiting amino acids in the hydrolysates, in that order.... |
Tipo: Text |
Palavras-chave: Cuttlefish protein hydrolysate (CPH); Amino acid; Enzyme; Sepia officinalis. |
Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00182/29285/27715.pdf |
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Cahu, Chantal; Zambonino, Jose-luis; Takeuchi, Toshio. |
Marine fish larvae undergo major functional and morphological changes during the developmental stages and several factors can interfere with the normal development of larvae and affect fry quality. Skeletal malformations, such as spinal malformation-scoliosis, lordosis, coiled vertebral column-, missing or additional fin rays, bending opercle or jaw malformations, are frequently observed in hatchery-reared larvae. This paper reviews the effects of some nutritional components on skeletal development in larvae of a number of fish species. In the dietary lipid fraction, for instance, it was proven that the phospholipid concentration affected the spinal malformation rate in sea bass fed a compound diet from mouth opening onwards. Phosphatidylinositol, in... |
Tipo: Text |
Palavras-chave: Retinoic acid; Ascorbic acid; Amino acid; Phospholipid; Peptides; Skeletal development; Quality; Fish larvae. |
Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-443.pdf |
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Soares da Silva Araújo,Cristiane; Martinez Baraldi Artoni,Silvana; Francelino Araújo,Lúcio; Jeferson Quirino Lousada,Mário; Mack Junqueira,Otto; Roque Rodriguez,Anita Isabel. |
Two experiments were conducted in order to evaluate the effects of different dietary amino acid and calcium profiles on the bone characteristics of two chicken strains (Avian Farms and Cobb) during the starter phase. At the end of each experiment, 2 chickens of each replicate were sacrificed and their tibiae were collected for analysis of following bone characteristics: bone measurements (weight, length and thickness of the compact and spongy layers) and bone density (bone densitometry). A total of 540 birds were used in each experiment, divided into fully randomized blocks with a 3 x 2 factorial schemes, i. e., 3 amino acid profiles (methionine, lysine and threonine ­ 100%, 125% and 150% of NRC recommendations, 1994) and 2 calcium levels (75% and... |
Tipo: Journal article |
Palavras-chave: Amino acid; Bone density; Broiler chickens; Calcium. |
Ano: 2003 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000200001 |
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Dairiki,Jony Koji; Borghesi,Ricardo; Dias,Carlos Tadeu dos Santos; Cyrino,José Eurico Possebon. |
The objective of this work was to determine the dietary lysine (DL) and dietary arginine (DA) requirements of dourado (Salminus brasiliensis), through dose-response trials using the amino acid profiles of whole carcasses as a reference. Two experiments were carried out in a completely randomized design (n=4). In the first experiment, groups of 12 feed-conditioned dourado juveniles (11.4±0.2 g) were stocked in 60 L cages placed in 300 L plastic indoor tanks in a closed circulation system. Fish were fed for 60 days on diets containing 1.0, 1.5, 2.0, 2.5, 3.0, or 3.5 % dietary lysine. In the second experiment, dourado juveniles (27.0±0.8 g) were fed for 60 days on semipurified diets containing arginine at 1.0, 1.5, 2.0, 2.5 or 3.0%, in similar conditions to... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amino acid; Dose-response; Dourado; Regression. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000800032 |
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Huang,Ai-xia; Li,Jin-jun; Shen,Jun-da; Tao,Zheng-rong; Ren,Jin-dong; Li,Guo-qing; Wang,De-qian; Tian,Yong; Yang,Fu-sheng; Ding,Wei-ping; Wu,Tian-xing; Lu,Li-zhi. |
We investigated the effects of crossbreeding on slaughter traits and the chemical composition of chicken breast muscle. Trials were conducted using 120 broilers from four lines: Xiao-Shan chicken (XS), Xian-Ju chicken (XJ), Xiao-Shan chicken♂♂ × Xian-Ju chicken♀♀ (Zhenan 1, ZNY1) and Xiao-Shan chicken♂♂ × (Guang-Xi Yellow chicken♂♂×Xian-Ju chicken♀♀) ♀♀ (Zhenan 2, ZNY2). The birds were slaughtered at 120 days of age and the slaughter traits were measured. Breast muscles were sampled to determine chemical composition. The slaughter traits of hybrid chickens were improved. Both hybrid strains had higher intramuscular fat (IMF) and inosine-5'-monophosphate (inosinic acid, IMP). Concentrations of monounsaturated fatty acids (MUFA) in breast muscles from the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amino acid; Chicken; Crossbreeding; Fatty acid; Inosine-5'-monophosphate; Intramuscular fat; Slaughter traits. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2011000400005 |
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Meirelles,HT; Albuquerque,R; Borgatti,LMO; Souza,LWO; Meister,NC; Lima,FR. |
One-day-old male Ross chicks were used in an experiment designed to compare two methionine sources, DL-methionine and methionine hydroxy analogue free acid (MHA-FA), and four different levels: 0.41; 0.47; 0.53; 0.59% (starter diet); 0.35; 0.41; 0.47; 0.53% (grower diet); and 0.30; 0.36; 0.42; 0.48% (finisher diet). One thousand two hundred and eighty chicks were housed in 32 experimental floor-pens (40 birds each) and fed 8 experimental diets based on corn and soybean meal for 47 days. The effects of methionine sources and levels were evaluated by performance data, carcass and cut yields, feather yield and abdominal fat content. Data were analyzed as a completely randomized design in a 2x4 factorial arrangement (2 sources and 4 levels), with 8 treatments... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amino acid; Broilers; Carcass yield; Methionine source; Performance. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2003000100009 |
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Maiorka,A; Dahlke,F; Santin,E; Kessler,AM; Penz Jr.,AM. |
This study was conducted to evaluate the performance and carcass and breast yields of broilers fed two different energy levels (3,200 or 2,900 kcal ME/kg) and two ways of expressing amino acid requirements (total - TAA or digestible - DAA) between 21 and 42 days of age. The results showed that broilers fed the diet containing 3,200 kcal ME/kg had higher weight gain (P=0.015), better feed conversion (P=0.001), and higher abdominal fat deposition (P=0.001) as compared to those fed the diet containing 2,900 kcal ME/kg. Diets formulated on DAA basis promoted higher weight gain (P=0.043), better feed conversion (P=0.010) and better conversion of ME intake into weight gain (P=0.007) as compared to those formulated on TAA basis. The results of this study suggest... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amino acid; Broilers; Energy. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200003 |
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Porto,ML; Givisiez,PEN; Saraiva,EP; Costa,FGP; Moreira Filho,ALB; Andrade,MFS; Brandão,PA; Guerra,RR. |
ABSTRACTThe objective of this study was to evaluate the effects of 1% dietary glutamic acid on the body weight, intestinal morphometry, and anti-Newcastle antibody titers of broiler chickens submitted to heat stress. One-d-old male broiler chicks (n=120) were distributed according to a 2 x 2 factorial design with two environmental temperatures (thermoneutral or heat stress) and two diets (with 0 or 1% glutamic acid). Heat stress temperature was constantly maintained (24h/day) 5 ºC higher than the thermoneutral temperature. Diets supplied the nutritional requirements of broilers in the pre-starter (1 to 7d) and starter (8 to 21d) phases. Birds were vaccinated against Newcastle disease on d 7 via eye drop. On days 5, 10, 15, and 20, individual body weight... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amino acid; Duodenum morphology; Environmental temperature; Humoral immunity; Heat stress; Performance.. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000300355 |
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Mendes,AS; Api,I; Silva,L; Silva,RTL; Sausen,L; Menezes,LFG; Morello,GM; Carvalho,EH. |
There has been a great evolution and improvement in the nutrition of poultry and lysine has been used the reference amino acid to increase animal protein deposition. Therefore, a meta-analysis was performed with the objective of studying the effect of different dietary lysine levels on the performance and carcass yield of broiler chickens. In total, 21 studies published in Brazil since 1999 were included. Bird performance considered daily weight gain (178 observations) and feed conversion ratio (188 observations) results. Carcass quality was evaluated relative to carcass yield (24 observations), abdominal fat (24 observations) and breast meat yield (30 observations). Data were checked for normality and were submitted to analysis of variance. Weight gain... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amino acid; Nutrition; Poultry production. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000400013 |
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Carvalho,FB; Stringhini,JH; Leandro,NSM; Café,M; Sousa,RF; Santos,BM. |
ABSTRACT Post-hatch delayed placement damages, physical and physiological development of broiler chicks. The objective of this study was to ascertain adequate levels of glutamine inclusion in post-hatch and pre-starter feed, in order to minimize the negative effects of post-hatch delayed placement on broiler chicks. The birds were distributed in a completely randomized design, with four treatments and five replicates of ten birds each. Four levels of glutamine supplementation (0, 1, 2 and 3%) were used in the pre-starter feed, which was given to the chicks in the transportation box and during the pre-starter phase. After 24 hours of access to this feed, and at 7 days of age, the chicks’ performance, yolk sac retraction, plasma glucose concentration, weight... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amino acid; Poultry; Delayed placement; Glucose; Histomorphometry. |
Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000100300 |
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Oliveira Filho,PA; Cruz,FGG; Rufino,JPF; Silva,EM; Viana Filho,GB; Silva,FM. |
ABSTRACT The present study aimed to determine the ideal requirement of digestible methionine + cystine to molted laying hens. The experimental period lasted 105 days, divided into five periods of 21 days. 144 Hisex White laying hens with 84 weeks-of-age were used. The experimental design was completely randomized with treatments constituted for six levels of digestible methionine + cystine (0.45, 0.50, 0.55, 0.60, 0.65, and 0.70%) in the diets, with four replicates of six birds each. Data collected were evaluated by polynomial regression at 5%. There was a significant effect (p<0.05) of digestible methionine + cystine on feed intake, energy intake, protein intake, egg production, and egg mass. Internal egg quality presented positive linear effect... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amino acid; Egg quality; Hen; Performance; Serum. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000400323 |
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Registros recuperados: 38 | |
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