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15N-labed glycine synthesis Anais da ABC (AABC)
Tavares,Claudinéia R.O.; Bendassolli,José A.; Coelho,Fernando; Sant'ana Filho,Carlos R.; Prestes,Clelber V..
This work describes a method for 15N-isotope-labeled glycine synthesis, as well as details about a recovery line for nitrogen residues. To that effect, amination of alpha-haloacids was performed, using carboxylic chloroacetic acid and labeled aqueous ammonia (15NH3). Special care was taken to avoid possible 15NH3 losses, since its production cost is high. In that respect, although the purchase cost of the 13N-labeled compound (radioactive) is lower, the stable tracer produced constitutes an important tool for N cycling studies in living organisms, also minimizing labor and environmental hazards, as well as time limitation problems in field studies. The tests were carried out with three replications, and variable 15NH3(aq) volumes in the reaction were used...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stable isotope; Glycine; Amino acid.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000300005
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N-terminal amino acids of bovine alpha interferons are relevant for the neutralization of their antiviral activity BJMBR
Barreto Filho,J.B.; Eiras,P.R.S.; Golgher,R.R..
The structure-function relationship of interferons (IFNs) has been studied by epitope mapping. Epitopes of bovine IFNs, however, are practically unknown, despite their importance in virus infections and in the maternal recognition of pregnancy. It has been shown that recombinant bovine (rBo)IFN-alphaC and rBoIFN-alpha1 differ only in 12 amino acids and that the F12 monoclonal antibody (mAb) binds to a linear sequence of residues 10 to 34. We show here that the antiviral activities of these two IFNs were neutralized by the F12 mAb to different extents using two tests. In residual activity tests the antiviral activity dropped by more than 99% with rBoIFN-alphaC and by 84% with rBoIFN-alpha1. In checkerboard antibody titrations, the F12 mAb titer was 12,000...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Bovine alpha interferon; Neutralization; Amino acid; Epitope.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500015
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A leucine-supplemented diet improved protein content of skeletal muscle in young tumor-bearing rats BJMBR
Gomes-Marcondes,M.C.C.; Ventrucci,G.; Toledo,M.T.; Cury,L.; Cooper,J.C..
Cancer cachexia induces host protein wastage but the mechanisms are poorly understood. Branched-chain amino acids play a regulatory role in the modulation of both protein synthesis and degradation in host tissues. Leucine, an important amino acid in skeletal muscle, is higher oxidized in tumor-bearing animals. A leucine-supplemented diet was used to analyze the effects of Walker 256 tumor growth on body composition in young weanling Wistar rats divided into two main dietary groups: normal diet (N, 18% protein) and leucine-rich diet (L, 15% protein plus 3% leucine), which were further subdivided into control (N or L) or tumor-bearing (W or LW) subgroups. After 12 days, the animals were sacrificed and their carcass analyzed. The tumor-bearing groups showed a...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Leucine; Amino acid; Walker 256 carcinosarcoma; Body composition; Skeletal muscle protein.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100017
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The role of γ -aminobutyric acid (Gaba) in somatic embryogenesis of Acca sellowiana Berg. (Myrtaceae) Braz. J. Plant Physiol.
Booz,Maristela Raitz; Kerbauy,Gilberto B.; Guerra,Miguel Pedro; Pescador,Rosete.
The γ-aminobutyric acid (Gaba) is a non-protein amino acid found in prokaryotes and eukaryotes. Its role in plant development has not been fully established. This study reports a quantification of the levels of endogenous Gaba, as well as investigation of its role in different stages of somatic embryogenesis in Acca sellowiana Berg. (Myrtaceae). Zygotic embryos were used as explants and they were inoculated into the culture medium contained different concentrations of Gaba (0,2, 4, 6, 8 and 10 µM). The highest concentrations of endogenous Gaba were detected between the third and nine days after inoculation, reaching the value of 12.77 µmol.g-1FW. High frequency of somatic embryogenesis was observed in response to 10 µM Gaba. This treatment also resulted in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid; Pineapple guava; Plant growth.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202009000400003
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The browning kinetics of the non-enzymatic browning reaction in L-ascorbic acid/basic amino acid systems Ciênc. Tecnol. Aliment.
YU,Ai-Nong; LI,Ya; YANG,Yan; YU,Ke.
Abstract Under the conditions of weak basis and the reaction temperature range of 110-150 °C, lysine, arginine and histidine were reacted with L-ascorbic acid at equal amount for 30-150 min, respectively and the formation of browning products was monitored with UV–vis spectrometry. The kinetic characteristics of their non-enzymatic browning reaction were investigated. The study results indicated that the non-enzymatic browning reaction of these three amino acids with L-ascorbic acid to form browning products was zero-order reaction. The apparent activation energies for the formation of browning products from L-ascorbic acid/lysine, L-ascorbic acid/arginine and L-ascorbic acid/histidine systems were 54.94, 50.08 and 35.31kJ/mol. The activation energy data...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Kinetics; Non-enzymatic browning reaction; Ascorbic acid; Maillard reaction; Amino acid.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612018000300537
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Sequences of digestible lysine for gilts from 60 to 148 days of age Ciência Rural
Silva Júnior,Veredino Louzada da; Donzele,Juarez Lopes; Oliveira,Rita Flávia Miranda de; Saraiva,Alysson; Silva,Francisco Carlos de Oliveira; Kill,João Luís.
The experiment was conducted to evaluate five nutritional plans based on sequences of standardized ileal digestible lysine: 0.90-0.80-0.70, 1.00-0.90-0.80, 1.10-1.00-0.90, 1.20-1.10-1.00, and 1.30-1.20-1.10% fed to gilts from 60 to 99, 129 to 100, and 130 to 148 days of age, respectively. Eighty commercial hybrid gilts, selected for lean gain, with initial weight of 23.46±0.27kg were allotted in a randomized block design, with five treatments, eight replicates, and two pigs per experimental unit. No effect (P>0.05) of the nutritional plans was verified on daily feed intake, daily weight gain and feed conversion. The nutritional plans had no influence (P>0.05) on any of the carcass traits evaluated (carcass yield, meat amount, and meat yield). The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid; Carcass; Performance; Pigs; Requirement.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000100118
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Specific nutrient supplementation of defined serum-free medium for the improvement of CHO cells growth and t-PA production Electron. J. Biotechnol.
Altamirano,Claudia; Illanes,Andrés; Canessa,Rossana; Becerra,Silvana.
Recombinant CHO TF70R cells are able to grow and produce t-PA on serum-free medium BIOPRO1 (BioWhitaker Europe, Belgium). The purpose of the present study was to determine the effect of medium supplementation with vitamins, lipids, and specific amino acids on cell growth, t-PA production and biological functionality. Among vitamins, only biotin, folic acid, cobalamine and benzoic acid were required for improving growth and t-PA production. Lipid supplement allowed a significant increase cell concentration and t-PA specific activity and concentration, though its specific production rate decreased slightly. Medium supplementation with proline, serine and asparagine had also positive effects on cell growth. Besides, the addition of asparagine (even in the...
Tipo: Journal article Palavras-chave: Amino acid; Cholesterol; Vitamins.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100009
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Optimization of common cuttlefish (Sepia officinalis) protein hydrolysate using Pepsin by Response Surface Methodology ArchiMer
Kechaou, Emna; Berge, Jean-pascal; Jaouen, Pascal; Ben Amar, Raja.
Protein hydrolysate was prepared from common cuttlefish Sepia officinalis by Pepsin. Hydrolysis conditions (time, temperature, and enzyme activity) were optimized by response surface methodology (RSM) using a factorial design. The regression coefficient was close to 0.999, observed during both experimental and validation runs, and indicated the validity of prediction model. An enzyme activity to substrate ratio (X1), 0.04 AU/g protein; time (X2), 85 minutes; and temperature (X3), 45 °C were found to be the optimum conditions for a higher degree of hydrolysis (21%) and nitrogen recovery (90%) using Pepsin. According to amino acid analysis results and chemical score, methionine and histidine are the limiting amino acids in the hydrolysates, in that order....
Tipo: Text Palavras-chave: Cuttlefish protein hydrolysate (CPH); Amino acid; Enzyme; Sepia officinalis.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00182/29285/27715.pdf
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Nutritional components affecting skeletal development in fish larvae ArchiMer
Cahu, Chantal; Zambonino, Jose-luis; Takeuchi, Toshio.
Marine fish larvae undergo major functional and morphological changes during the developmental stages and several factors can interfere with the normal development of larvae and affect fry quality. Skeletal malformations, such as spinal malformation-scoliosis, lordosis, coiled vertebral column-, missing or additional fin rays, bending opercle or jaw malformations, are frequently observed in hatchery-reared larvae. This paper reviews the effects of some nutritional components on skeletal development in larvae of a number of fish species. In the dietary lipid fraction, for instance, it was proven that the phospholipid concentration affected the spinal malformation rate in sea bass fed a compound diet from mouth opening onwards. Phosphatidylinositol, in...
Tipo: Text Palavras-chave: Retinoic acid; Ascorbic acid; Amino acid; Phospholipid; Peptides; Skeletal development; Quality; Fish larvae.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-443.pdf
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BONE DEVELOPMENT OF BROILER CHICKENS FED DIETS WITH DIFFERENT AMINO ACID AND CALCIUM LEVELS DURING THE STARTER PHASE International Journal of Morphology
Soares da Silva Araújo,Cristiane; Martinez Baraldi Artoni,Silvana; Francelino Araújo,Lúcio; Jeferson Quirino Lousada,Mário; Mack Junqueira,Otto; Roque Rodriguez,Anita Isabel.
Two experiments were conducted in order to evaluate the effects of different dietary amino acid and calcium profiles on the bone characteristics of two chicken strains (Avian Farms and Cobb) during the starter phase. At the end of each experiment, 2 chickens of each replicate were sacrificed and their tibiae were collected for analysis of following bone characteristics: bone measurements (weight, length and thickness of the compact and spongy layers) and bone density (bone densitometry). A total of 540 birds were used in each experiment, divided into fully randomized blocks with a 3 x 2 factorial schemes, i. e., 3 amino acid profiles (methionine, lysine and threonine ­ 100%, 125% and 150% of NRC recommendations, 1994) and 2 calcium levels (75% and...
Tipo: Journal article Palavras-chave: Amino acid; Bone density; Broiler chickens; Calcium.
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000200001
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Effects of various nutrients and renewal of medium on in vitro development of bovine oocytes matured and fertilized in vitro OAK
FUKUI, Yutaka; LEE, Eun Song; KANEZAWA, Yuka; 福井, 豊; 李, 殷松; 金澤, 由佳.
http://www.obihiro.ac.jp/~library/kenkyu.html
Palavras-chave: Amino acid; Glucose; In vitro development; Oocyte; Cattle.
Ano: 1995 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3790
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Lysine and arginine requirements of Salminus brasiliensis PAB
Dairiki,Jony Koji; Borghesi,Ricardo; Dias,Carlos Tadeu dos Santos; Cyrino,José Eurico Possebon.
The objective of this work was to determine the dietary lysine (DL) and dietary arginine (DA) requirements of dourado (Salminus brasiliensis), through dose-response trials using the amino acid profiles of whole carcasses as a reference. Two experiments were carried out in a completely randomized design (n=4). In the first experiment, groups of 12 feed-conditioned dourado juveniles (11.4±0.2 g) were stocked in 60 L cages placed in 300 L plastic indoor tanks in a closed circulation system. Fish were fed for 60 days on diets containing 1.0, 1.5, 2.0, 2.5, 3.0, or 3.5 % dietary lysine. In the second experiment, dourado juveniles (27.0±0.8 g) were fed for 60 days on semipurified diets containing arginine at 1.0, 1.5, 2.0, 2.5 or 3.0%, in similar conditions to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid; Dose-response; Dourado; Regression.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000800032
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Chemical composition and fatty acid contents in farmed freshwater prawns PAB
Portella,Carolina de Gasperi; Sant'Ana,Léa Silvia; Valenti,Wagner Cotroni.
The objective of this work was to evaluate the chemical composition and fatty acid contents of Amazonian and giant river prawns. After four-month farming, with the same diet for both species, palmitic and stearic acids were the main saturated fatty acids. Oleic acid was the main monounsatured fatty acid, and the eicosapentaenoic and docosahexaenoic acids were the most abundant polyunsaturated acids. Amazonian prawn has higher levels of protein and polyunsaturated fatty acids than those of the giant river prawn, which shows its potential for aquaculture.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Macrobrachium amazonicum; Macrobrachium rosenbergii; Amino acid; Fatty acids; Meat quality.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000800046
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Effects of crossbreeding on slaughter traits and breast muscle chemical composition in chinese chickens Rev. Bras. Ciênc. Avic.
Huang,Ai-xia; Li,Jin-jun; Shen,Jun-da; Tao,Zheng-rong; Ren,Jin-dong; Li,Guo-qing; Wang,De-qian; Tian,Yong; Yang,Fu-sheng; Ding,Wei-ping; Wu,Tian-xing; Lu,Li-zhi.
We investigated the effects of crossbreeding on slaughter traits and the chemical composition of chicken breast muscle. Trials were conducted using 120 broilers from four lines: Xiao-Shan chicken (XS), Xian-Ju chicken (XJ), Xiao-Shan chicken♂♂ × Xian-Ju chicken♀♀ (Zhenan 1, ZNY1) and Xiao-Shan chicken♂♂ × (Guang-Xi Yellow chicken♂♂×Xian-Ju chicken♀♀) ♀♀ (Zhenan 2, ZNY2). The birds were slaughtered at 120 days of age and the slaughter traits were measured. Breast muscles were sampled to determine chemical composition. The slaughter traits of hybrid chickens were improved. Both hybrid strains had higher intramuscular fat (IMF) and inosine-5'-monophosphate (inosinic acid, IMP). Concentrations of monounsaturated fatty acids (MUFA) in breast muscles from the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid; Chicken; Crossbreeding; Fatty acid; Inosine-5'-monophosphate; Intramuscular fat; Slaughter traits.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2011000400005
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Performance of broilers fed with different levels of methionine hydroxy analogue and DL-methionine Rev. Bras. Ciênc. Avic.
Meirelles,HT; Albuquerque,R; Borgatti,LMO; Souza,LWO; Meister,NC; Lima,FR.
One-day-old male Ross chicks were used in an experiment designed to compare two methionine sources, DL-methionine and methionine hydroxy analogue free acid (MHA-FA), and four different levels: 0.41; 0.47; 0.53; 0.59% (starter diet); 0.35; 0.41; 0.47; 0.53% (grower diet); and 0.30; 0.36; 0.42; 0.48% (finisher diet). One thousand two hundred and eighty chicks were housed in 32 experimental floor-pens (40 birds each) and fed 8 experimental diets based on corn and soybean meal for 47 days. The effects of methionine sources and levels were evaluated by performance data, carcass and cut yields, feather yield and abdominal fat content. Data were analyzed as a completely randomized design in a 2x4 factorial arrangement (2 sources and 4 levels), with 8 treatments...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid; Broilers; Carcass yield; Methionine source; Performance.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2003000100009
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Effect of energy levels of diets formulated on total or digestible amino acid basis on broiler performance Rev. Bras. Ciênc. Avic.
Maiorka,A; Dahlke,F; Santin,E; Kessler,AM; Penz Jr.,AM.
This study was conducted to evaluate the performance and carcass and breast yields of broilers fed two different energy levels (3,200 or 2,900 kcal ME/kg) and two ways of expressing amino acid requirements (total - TAA or digestible - DAA) between 21 and 42 days of age. The results showed that broilers fed the diet containing 3,200 kcal ME/kg had higher weight gain (P=0.015), better feed conversion (P=0.001), and higher abdominal fat deposition (P=0.001) as compared to those fed the diet containing 2,900 kcal ME/kg. Diets formulated on DAA basis promoted higher weight gain (P=0.043), better feed conversion (P=0.010) and better conversion of ME intake into weight gain (P=0.007) as compared to those formulated on TAA basis. The results of this study suggest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid; Broilers; Energy.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200003
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GLUTAMIC ACID IMPROVES BODY WEIGHT GAIN AND INTESTINAL MORPHOLOGY OF BROILER CHICKENS SUBMITTED TO HEAT STRESS Rev. Bras. Ciênc. Avic.
Porto,ML; Givisiez,PEN; Saraiva,EP; Costa,FGP; Moreira Filho,ALB; Andrade,MFS; Brandão,PA; Guerra,RR.
ABSTRACTThe objective of this study was to evaluate the effects of 1% dietary glutamic acid on the body weight, intestinal morphometry, and anti-Newcastle antibody titers of broiler chickens submitted to heat stress. One-d-old male broiler chicks (n=120) were distributed according to a 2 x 2 factorial design with two environmental temperatures (thermoneutral or heat stress) and two diets (with 0 or 1% glutamic acid). Heat stress temperature was constantly maintained (24h/day) 5 ºC higher than the thermoneutral temperature. Diets supplied the nutritional requirements of broilers in the pre-starter (1 to 7d) and starter (8 to 21d) phases. Birds were vaccinated against Newcastle disease on d 7 via eye drop. On days 5, 10, 15, and 20, individual body weight...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid; Duodenum morphology; Environmental temperature; Humoral immunity; Heat stress; Performance..
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000300355
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Effects of dietary lysine on broiler performance and carcass yield - meta-analysis Rev. Bras. Ciênc. Avic.
Mendes,AS; Api,I; Silva,L; Silva,RTL; Sausen,L; Menezes,LFG; Morello,GM; Carvalho,EH.
There has been a great evolution and improvement in the nutrition of poultry and lysine has been used the reference amino acid to increase animal protein deposition. Therefore, a meta-analysis was performed with the objective of studying the effect of different dietary lysine levels on the performance and carcass yield of broiler chickens. In total, 21 studies published in Brazil since 1999 were included. Bird performance considered daily weight gain (178 observations) and feed conversion ratio (188 observations) results. Carcass quality was evaluated relative to carcass yield (24 observations), abdominal fat (24 observations) and breast meat yield (30 observations). Data were checked for normality and were submitted to analysis of variance. Weight gain...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid; Nutrition; Poultry production.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000400013
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Use of Glutamine in Post-Hatch Feed for Broiler Chicks Rev. Bras. Ciênc. Avic.
Carvalho,FB; Stringhini,JH; Leandro,NSM; Café,M; Sousa,RF; Santos,BM.
ABSTRACT Post-hatch delayed placement damages, physical and physiological development of broiler chicks. The objective of this study was to ascertain adequate levels of glutamine inclusion in post-hatch and pre-starter feed, in order to minimize the negative effects of post-hatch delayed placement on broiler chicks. The birds were distributed in a completely randomized design, with four treatments and five replicates of ten birds each. Four levels of glutamine supplementation (0, 1, 2 and 3%) were used in the pre-starter feed, which was given to the chicks in the transportation box and during the pre-starter phase. After 24 hours of access to this feed, and at 7 days of age, the chicks’ performance, yolk sac retraction, plasma glucose concentration, weight...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid; Poultry; Delayed placement; Glucose; Histomorphometry.
Ano: 2021 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000100300
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Requirement of Digestible Methionine + Cystine to Molted Laying Hens Rev. Bras. Ciênc. Avic.
Oliveira Filho,PA; Cruz,FGG; Rufino,JPF; Silva,EM; Viana Filho,GB; Silva,FM.
ABSTRACT The present study aimed to determine the ideal requirement of digestible methionine + cystine to molted laying hens. The experimental period lasted 105 days, divided into five periods of 21 days. 144 Hisex White laying hens with 84 weeks-of-age were used. The experimental design was completely randomized with treatments constituted for six levels of digestible methionine + cystine (0.45, 0.50, 0.55, 0.60, 0.65, and 0.70%) in the diets, with four replicates of six birds each. Data collected were evaluated by polynomial regression at 5%. There was a significant effect (p<0.05) of digestible methionine + cystine on feed intake, energy intake, protein intake, egg production, and egg mass. Internal egg quality presented positive linear effect...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid; Egg quality; Hen; Performance; Serum.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000400323
Registros recuperados: 38
Primeira ... 12 ... Última
 

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