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Registros recuperados: 37 | |
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Ma, Hongming; Cahu, Chantal; Zambonino, Jose-luis; Yu, Hairui; Duan, Qingyuan; Le Gall, Marie-madeleine; Mai, Kangsen. |
Large yellow croaker is an important marine fish species for aquaculture in China. Larval rearing of this species is still based on live prey feeding. The aim of this study was to describe the onset and development of the main digestive enzymes during larval development of this species, in order to obtain essential data for the formulation of a compound diet adapted to larvae. Enzymatic assays were conducted from day I post hatching to day 40 in larvae fed the following live prey sequence: rotifers, Artemia nauplii, copepods, and then frozen copepods. Assays were done on whole larvae until day 19, then on dissected larvae and on purified brush border membranes of intestine. Pancreatic enzymes, amylase, and trypsin, showed high activities from early stages,... |
Tipo: Text |
Palavras-chave: Trypsin; Pseudosciaena crocea; Fish larval development; Amylase; Aminopeptidase N; Alkaline phosphatase. |
Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-444.pdf |
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Degremont, Lionel. |
L'analyse du polymorphisme des gènes de J'amylase chez Crassostrea gigas (Thunberg, 1793) a été établi sur deux générations dans le cadre du programme européen GENEPHYS (génétique et physiologie chez C.gigas). Après une amplification de l'ADN par PCR à partir de deux couples d'amorces qui sont spécifiques à chacun des deux gènes de l'amylase, une digestion par EcoRI de l'ADN amplifié suivie d'une électrophorése en gel d'agarose sont effectuées. Cette technique (RFLP) permet de déterminer le polymorphisme génétique des huîtres creuses. Une troisième génération a été réalisée afin d'obtenir quatre familles et d'en analyser le génotype. Ces familles ont été tout d'abord placées pendant cinq mois dans un milieu riche en nourriture, puis ensuite, chaque famille... |
Tipo: Text |
Palavras-chave: Crassostrea gigas; Amylase; Polymorphisme; Croissance; Efficacité d'assimilation. |
Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/00439/55109/56561.pdf |
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Tovar Ramirez, Dariel; Zambonino-infante, Jose-luis; Cahu, Chantal; Gatesoupe, Joel; Vazquez Juarez, R. |
Recent results have demonstrated that live yeast in diets improved gut maturation in sea bass larvae. In this study, we tested the absence and two levels of live yeast in sea bass larvae diets. Specimens were fed from time of mouth opening to 37 days after hatching a diet of 0%, 1.1%, or 5.7% wet weight of live yeast (Debaryomyces hansenii CBS 8339). Yeast incorporation improved survival 10%, and reduced malformed larvae. In groups fed 1.1% yeast, only 1% of larvae were malformed, compared to 14% in the control group. Final mean weight in groups fed 1.1% yeast was twice that of other groups. Activities and concentrations of mRNA trypsin and lipase were higher in the two groups fed yeast than in the control group, whereas activity and concentration of mRNA... |
Tipo: Text |
Palavras-chave: Polyamines; Lipase; Trypsin; Amylase; Enzymes; Gut maturation; Fish larvae. |
Ano: 2004 |
URL: https://archimer.ifremer.fr/doc/2004/publication-831.pdf |
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Sauvage, Christopher. |
In natural population of the Pacific cupped oyster, there are differences between allelic and genotypic frequencies for the A and B amylase genes. Set up a synthetical population of C. gigas, in which whole alleles and genotypes are better represented than in wild population, was done. An investigation links between trophic conditions applied during larvae developpment (in nursery), allelic frequencies for both amylase genes, oyster's individual waight and by the selectivity of summer mortalities during their exposition on the estran has been done. As a result, following facts have been observed : - On one side, an influence of the trophic condition applied during the larvae development on genotypic frequencies of the gene B of the amylase - On the other... |
Tipo: Text |
Palavras-chave: Crassostrea gigas; Amylase; Polymorphisme; Condition trophique; Crassostre gigas; Amylase; Polymorphim; Trophic condition. |
Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00371/48185/48300.pdf |
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Rambelo-ramanitra, Seheno. |
Abnormal massive mortalities of oysters Crassostrea gigas during the estival period are a phenonmenon described in the literature since forty years. A certain number of studies show that it could exist a genectic base with these mortalities. In front of the economic interest that the oyster production in France represents, IFREMER set up program MOREST, gathering the fiels of physiology, pathology and the genetics. This study in interested in rare genotypes, determined by PCR-RFLP, presenting three bands for one or two A and B amylase genes, met in the families MOREST and the polymorphic population for amylase genes, made up starting from crossing of the hierarchical type. It aims at making a follow-up of the segregation of the alleles at the progeny, as... |
Tipo: Text |
Palavras-chave: Crassostrea gigas; Extraction d'ADN; Amylase; Polymorphisme; Génotypes; Crassostrea gigas; DNA Extraction; Amylase; Polymorphism; Genotypes. |
Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/00371/48230/48364.pdf |
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Dourado,LRB; Sakomura,NK; Barbosa,NAA; Bonato,MA; Kawuauchi,IM; Fernandes,JBK; Costa,FGP. |
Two metabolism assays were carried out to determine corn and soybean meal metabolizable energy when enzymes were added. In the first trial, 35 cockerels per studied feedstuff (corn and soybean meal) were distributed in a completely randomized experimental design with four treatments of seven replicates of one bird each. The evaluated treatments were: ingredient (corn and soybean meal) with no enzyme addition, with the addition of an enzyme complex (xylanase, amylase, protease - XAP), xylanase, or phytase. Precise feeding method was used to determine true metabolizable energy corrected for nitrogen balance (TMEn). The use of enzymes did not result in any differences (p>0.05) in soybean meal TMEn, but phytase improved corn TMEn in 2.3% (p=0.004). In the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amylase; Forced feeding; Phytase; Protease; Xylanase. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000100008 |
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Neumann,Mikael; Leão,Guilherme Fernando Mattos; Horst,Egon Henrique; Stuani,Osmair Flavio; Sangali,Cleiton Pagliari; Castilho,Ricardo. |
ABSTRACT The objective of this study was to evaluate the effects of supplementation of two different enzymatic complexes, fibrolytic (NSPases) or amylolytic (EXP3066) enzymes, on growth performance, digestibility, behavior, and carcass characteristics of feedlot cattle. Thirty-six ½ Angus yearling bulls with an average initial weight of 391±5.0 kg were used in this experiment blocked by weight in a completely randomized design divided in three treatments: control, NSPases, and EXP3066. The basal diet was composed of 85% whole corn grain and 15% nucleus. NSPases increased average daily gain on days 63 and 84, and gain:feed ratio on days 42, 63, and 84. Total carcass weight and carcass daily gain were improved by 4.8% and up to 6.0% with EXP3066 and NSPases... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amylase; Corn; High-energy diet; Non-starch polysaccharides; Xylanase. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100611 |
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Oliveira, Helena; Universidade de Aveiro; Costa, Armando; Universidade de Aveiro; Santos, Conceição; Universidade de Aveiro. |
Vineyards production is often affected by diseases as esca and Petri disease and by excess of salt. Phaeomoniella chlamydospora is one of the pathogenic fungi associated to esca disease, but its interaction with the plant under excess of salt remains unknown. Under controlled in vitro conditions, Vitis vinifera L. plants were exposed to 0, 20 and 100 mM NaCl and inoculated with P. chlamydospora. Both inoculation and salt stress decreased the levels of chlorophylls, which was aggravated when both factors were combined. NaCl 100 mM and, mostly, inoculation, decreased maximum fluorescence (Fm), variable fluorescence (Fv) and Fv/Fm. The activity of a-amylase decreased in plants exposed to 100 mM or inoculated but no synergic effect of these factors was... |
Tipo: Ensaio laboratorial em condições controladas |
Palavras-chave: 5.01.02.01-0; 5.01.03.06-7 Fisiologia de Plantas grapevine; Phaeomoniella chlamydospora; Salt stress; Starch metabolism; Amylase; Sucrose metabolism. |
Ano: 2013 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15690 |
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Diaz,G.J.; Vargas,M.L.; Cortés,A.. |
ABSTRACT A trial was conducted to evaluate a feed additive containing epoxidase activity from a bacterium (Mycofix-S) as a potential protection against the adverse effects of 2.5 ppm dietary T-2 toxin in male growing broiler chickens. A total of 144 one-day-old Ross 308 male chicks were individually wing-banded and allotted into each of the four experimental groups. Group 1: negative control, no T-2 toxin or additive; group 2: Mycofix-S, 2.5 g/kg; group 3: positive control, 2.5 ppm T-2 toxin; group 4: 2.5 ppm T-2 toxin + 2.5 g/kg Mycofix-S. Feed and water were provided ad libitum for 28 days (days 1 to 28 of age). Each experimental treatment was replicated 6 times, with 6 birds per replicate pen. Response variables included performance parameters, serum... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ALP; Amylase; Epoxidase; Mycotoxin decontamination; Trichothecenes. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000300709 |
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Almeida,Paula Zaghetto de; Pereira,Marita Gimenez; Carvalho,Caio Cesar de; Heinen,Paulo Ricardo; Ziotti,Luciana Sobrani; Messias,Josana Maria; Jorge,João Atilio; Polizeli,Maria de Lourdes Teixeira de Moraes. |
Abstract Filamentous fungi are widely diverse and ubiquitous organisms. Such biodiversity is barely known, making room for a great potential still to be discovered, especially in tropical environments - which are favorable to growth and species variety. Filamentous fungi are extensively applied to the production of industrial enzymes, such as the amylases. This class of enzymes acts in the hydrolysis of starch to glucose or maltooligosaccharides. In this work twenty-five filamentous fungi were isolated from samples of decomposing material collected in the Brazilian Atlantic Forest. The two best amylase producers were identified as Aspergillus brasiliensis and Rhizopus oryzae. Both are mesophilic, they grow well in organic nitrogen-rich media produce great... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amylase; Filamentous fungi; Aspergillus brasiliensis; Rhizopus oryzae; Glucoamylase; Bioprospection. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032017000300204 |
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Katoch,Meenu; Salgotra,Aseem; Singh,Gurpreet. |
This study aimed to screen the endophytic fungal species of ethano-medicinal plant Bacopa monnieri (L.) Pennell for their ability to produce antimicrobial substances against Bacillus subtilis, Pseudomonas aeroginosa, Salmonella typhimurium, Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, and Candida albicans. Endophytes were also screened for their ability to produce amylase, cellulase, protease and lipase to evaluate their ecological role within the host plant. Twenty-six endophytes were isolated and seventeen were identified. All the isolated endophytes exhibited amylolytic activity. Lipolytic, cellulolytic, proteolytic activity was shown by 98, 28 and 31% isolates, respectively. Similarly, all the endophytes (100%) exhibited significant... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacopa monnieri; Antimicrobial; ITS 5.8S rDNA; Lipase; Amylase; Endophyte. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500714 |
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Lacerda,Luiz Gustavo; Almeida,Rafael Ramires; Demiate,Ivo Mottin; Carvalho Filho,Marco Aurélio Silva; Vasconcelos,Eliane Carvalho; Woiciechowski,Adenise Lorenci; Bannach,Gilbert; Schnitzler,Egon; Soccol,Carlos Ricardo. |
Starch nutritional fractions as well as thermal properties and other analysis are essential for food and industrial application. Cassava bagasse is an important agro-industrial residue and its starch content was evaluated using two alternative methods. Thermal characterization and microscopy analyses helped to understand how hydrolysis digests starchy fraction of cassava bagasse. The melting point of cassava starch occurred at 169.2ºC. Regarding TG analyses, after moisture content, there were observed two main mass losses for all samples. Results suggest hydrolysis carried out using enzyme is less effective in order to convert total starch content in cassava bagasse. However, using sulfuric acid, fibers are affected by analyses conditions. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amylase; Cassava; Hydrolysis; Thermal analysis. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700019 |
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Lacerda,Luiz Gustavo; Azevedo,Jayme Augusto Menegassi; Carvalho Filho,Marco Aurélio da Silva; Demiate,Ivo Mottin; Schnitzler,Egon; Vandenberghe,Luciana Porto de Souza; Soccol,Carlos Ricardo. |
Cassava starch, partially hydrolyzed by fungal á-amylase, was characterized using thermal analysis, light microscopy and X-ray diffraction. Thermal degradation was initiated at lower degradation temperatures after enzymatic treatment and the DSC (Differential scanning calorimetry) analysis showed almost similar range of gelatinization temperature, but the enthalpies of gelatinization were quite increased for the partially hydrolyzed starch granules. The results suggested that the partial degradation of the starch granules was concentrated in the amorphous regions. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amylase; Cassava; Hydrolysis; Thermal analysis. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600016 |
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Registros recuperados: 37 | |
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