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Reproduction uniparentale et polyploidie induites chez la truite arc-en-ciel (Oncorhynchus mykiss) et chez les bivalves Crassostrea gigas, Ruditapes philippinarum et Chlamys varia ArchiMer
Diter, Alain.
Methods of gynogenesis, androgenesis and polyploidy induction were developed with the aim of genetic improvement in the rainbow trout, the Pacific oyster, the Manila clam and the black scallop. In the trout: · haploid androgenesis was induced by means of a pressure shock applied on diploid eggs, probably at the time of karyogamy. Diploid androgenetic individuals were directly produced from eggs fertilized with sperm from tetraploid male. · a heat shock treatment effective for suppressing the first egg mitosis was developed. This treatment allows the production of homozygous gynogenetic lines (checked using enzyme electrophoresis technique) from eggs inseminated with ultraviolet irradiated sperm. The treatment also induced tetraploidy in eggs normally...
Tipo: Text Palavras-chave: Méthode induction gynogenèse; Androgenèse; Polyploïdie; Oncorhynchus mykiss; Crassostrea gigas; Ruditapes philippinarum; Chlamys varis; Génétique; Methods of gynogenesis; Androgenesis; Polyploidy; Oncorhynchus mykiss; Crassostrea gigas; Ruditapes philippinarum; Chlamys varis; Genetic.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/00210/32097/30553.pdf
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Towards the development of clonal lines in the European sea bass (Dicentrarchus labrax L.): application of uniparental reproduction techniques with an insight into sea bass eggs ArchiMer
Colleter, Julie.
Clonal lines are a powerful scientific tool for improved genetic characterization of organisms used in research. Inbred fish lines can be produced in only two generations using uniparental reproduction techniques. Androgenesis, achieved with variable success in several freshwater species, has been attempted in the European sea bass (Dicentrarchus labrax L), a marine fish of commercial and scientific interest. The low yields of progenies inheriting only the paternal genome after UV-irradiation of eggs led to considerations on the occurrence of UV screening compounds in pelagic eggs. Mycosporine-like amino acids and gadusol were found in many marine and freshwater organisms, but their occurrence in fish eggs was not clearly related to a behavioral pattern...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Lignées clonales; Androgenèse; Gynogenèse; MAAs; Gadusol; Marqueurs microsatellites; Homozygotie; Hétérozygotie; Taux de recombinaison.; Dicentrarchus labrax; Clonal lines; Androgenesis; Gynogenesis; MAAs; Gadusol; Microsatellite markers; Homozygosity; Heterozygosity; Recombination rate..
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00300/41138/40326.pdf
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