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Profile and regulation of annexin II expression during early embryogenesis in cattle Arq. Bras. Med. Vet. Zootec.
Costa,L.F.S.; Machado,M.S.N; Oliveira,J.F.C.; Silva,J.C.; Loguercio,R.S.; Gonçalves,P.B.D..
The presence of annexin II (Ann-II) during the initial stages of bovine embryo development and the regulation of Ann-II expression by retinol and insulin-like growth factor I (IGF-I) were studied. Bovine embryos at different stages of development were produced in vitro on Synthetic Oviductal Fluid (SOF) medium (control group), SOF supplemented with retinol (retinol group; 0.1ng/ml), or IGF-I (IGF-I group; 10ng/ml). The embryos were processed for mRNA extraction, cDNA production and polymerase chain reaction (PCR) using Ann-II-specific oligonucleotides. Ann-II was detected in all stages of early embryo development, except for the 16-cell stage. The blastocyst rates were significantly higher (P<0.05) in the group supplemented with retinol (37.8%, 45/119)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle; Annexin II; Embryo development.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000600023
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Annexin II mRNA expression in bovine oocytes during follicular development Genet. Mol. Biol.
Costa,Luis Fabiano Santos da; Machado,Márcia Silveira Netto; Oliveira,João Francisco Coelho de; Zamberlan,Gustavo; Gonçalves,Paulo Bayard Dias.
We investigated the expression of calcium-dependent phospholipid binding protein annexin-II (Ann-II) messenger RNA (mRNA) during preantral follicle development and in oocytes from antral follicles of different diameters (< 3 mm, 5 to 8 mm and > 8 mm). The action of retinol on Ann-II mRNA expression in mature oocytes was also examined. Only oocytes from secondary preantral follicles expressed Ann-II mRNA and at the germinal vesicle stage expression by oocytes from follicles larger than 8 mm was significantly higher (p < 0.05) compared with oocytes from follicles smaller than 3 mm or between 5 and 8 mm. Ann-II mRNA expression by metaphase II oocytes from follicles larger than 8 mm was significantly higher (p < 0.05) than that from oocytes from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine oocytes; Annexin II; Retinol.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200031
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