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Cultivable bacteria isolated from apple trees cultivated under different crop systems: Diversity and antagonistic activity against Colletotrichum gloeosporioides Genet. Mol. Biol.
Passos,João Frederico M. dos; Costa,Pedro B. da; Costa,Murilo D.; Zaffari,Gilmar R.; Nava,Gilberto; Boneti,José Itamar; Oliveira,Andréia Mara R. de; Passaglia,Luciane M.P..
This study evaluated the diversity of cultivable plant growth-promoting (PGP) bacteria associated with apple trees cultivated under different crop management systems and their antagonistic ability against Colletotrichum gloeosporioides. Samples of roots and rhizospheric soil from apple trees cultivated in organic and conventional orchards in southern Brazil were collected, together with soil samples from an area never used for agriculture (native field). Bacteria were identified at the genus level by PCR-RFLP and partial sequencing of the 16S rRNA, and were evaluated for some PGP abilities. The most abundant bacterial genera identified were Enterobacter (27.7%), Pseudomonas (18.7%), Burkholderia (13.7%), and Rahnella (12.3%). Sixty-nine isolates presented...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant growth-promoting bacteria; Apple tree; Antagonistic activity; Colletotrichum gloeosporioides.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400013
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Antimicrobial activity of the Nisin Z producer Lactococcus lactis subsp. lactis Lc08 against Listeria monocytogenes in skim milk Arq. Bras. Med. Vet. Zootec.
Perin,L.M.; Miranda,R.O.; Camargo,A.C.; Colombo,M.; Carvalho,A.F.; Nero,L.A..
The presented study aimed to verify the effect of different pH values, enzyme solutions and heat treatments on the antimicrobial activity of the bacteriocinogenic strain Lactococcus lactis subsp. lactis Lc08 and to test their antimicrobial activity against Listeria monocytogenes in reconstituted skim milk at refrigeration temperatures. This strain was previously described as a nisin Z producer and capable of inhibiting L. monocytogenes growth in in vitro tests. The antimicrobial activity of the bacteriocin cell-free supernatant of Lc08 was sensitive to enzyme treatments (except papain). The pH values and heating (65ºC for 30min, 75ºC for 15s) had no apparent effect on the antimicrobial activity of the bacteriocin produced by Lc08. Only treatment at...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nisin Z; Milk; Antagonistic activity; Lactococcus lactis subsp. lactis; Listeria monocytogenes.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000500037
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Antagonistic activity of antibiotic producing Streptomyces sp. against fish and human pathogenic bacteria BABT
Hossain,Nazmul; Rahman,Mahbubur.
In this study, attempts were made to isolate Streptomyces sp. from soil samples of two different regions of Bangladesh and evaluate their antagonistic activity against fish and human pathogenic bacteria. A total of 10 isolates were identified as Streptomyces sp. based on several morphological, physiological and biochemical tests. Cross streak method was used to observe the antagonistic activity of the Streptomyces sp. isolates against different fish pathogens belonging to the genus Aeromonas, Pseudomonas and Edwardsiella and human clinical isolates belonging to the genus Klebsiella, Salmonella and Streptococcus. Seven Streptomyces sp. isolates showed antagonism against both fish and human pathogenic bacteria. Four isolates viz., N24, N26, N28 and N47...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces sp.; Antagonistic activity; Cross streak method; Antibiotic.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200011
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In vitro Antimicrobial Assay of Actinomycetes in Rice AgainstXanthomonas oryzae pv. oryzicola and as Potential Plant Growth Promoter BABT
Hata,Erneeza Mohd; Sijam,Kamaruzaman; Ahmad,Zainal Abidin Mior; Yusof,Mohd Termizi; Azman,Noor Aisyah.
ABSTRACT The aim of this work was to invitro assay the antimicrobial activity of actinomycetes in rice against Xanthomonas oryzae pv. oryzicola and as potential plant growth promoter. A total of 92 actinomycete strains were isolated from different rice plant components and field locations. Of these, only 21.74% showed antagonistic activity against the Xoc pathogen. Molecular identification via 16s rRNA amplification revealed that 60% of the active antagonistic strains belonged to the genus Streptomyces. Isolates that demonstrated the highest antagonistic activity were also able to produce hydrolytic enzymes and plant growth-promoting hormones. Combination of preliminary screening based on in vitro antagonistic, hydrolytic enzyme and plant growth hormone...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antagonistic activity; Hydrolytic enzyme; Plant growth-promoting agent; Streptomyces.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600821
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Identification and biological activity of potential probiotic bacterium isolated from the stomach mucus of breast-fed lamb BJM
Sepová,H. Kiňová; Dubničková,M; Bilková,A; Bukovský,M; Bezáková,L.
The lactic acid bacterium E isolated from the stomach mucus of breast-fed lamb was identified by sequencing of 16S rDNA fragment and species-specific PCR as Lactobacillus reuteri. Its potential antimicrobial activity and ability to modulate immune system in vitro and in vivo was determined. The growth inhibition of potential pathogens decreased from Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella enterica ser. Minnesota to Escherichia coli. The lowest inhibition activity was observed in the case of Candida albicans. The ability of L. reuteri E to modulate biological activities of human and mouse mononuclear cells was estimated in vitro and in vivo, respectively. The production of IL-1β by monocytes in vitro was significantly induced by L. reuteri...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus reuteri; Mononuclear cells; PCR; Antagonistic activity; Phagocytosis.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300044
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