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Improvement of the indirect hemagglutination test for the detection of antibodies to Streptococcus pyogenes BJMBR
Rubinsky-Elefant,G.; Hoshino-Shimizu,S.; Mamizuka,E.M.; Asciutti,M.M.R..
An indirect hemagglutination test for a seroepidemiological survey of Streptococcus pyogenes infection was standardized. This is an improved modification of the indirect hemagglutination test which utilizes an unstable reagent prepared with fresh blood cells. Two types of bacterial antigens represented by extracellular products and purified streptolysin O were assayed, but only the former antigen gave good results. Pretreatment of the bacterial antigen with 0.15 M NaOH and neutralization to pH 5.5, as well as postfixation of sensitized red cells with 0.1% glutaraldehyde at 56oC for 30 min were found to be essential to give long stability to the reagent in liquid suspension, at least 9 months at 4oC. A total of 564 serum samples with high, moderate and low...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptococcus pyogenes; Hemagglutination test; Anti-streptolysin O antibodies.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800008
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