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Antimicrobial activity of honeys from two stingless honeybee species and Apis mellifera (Hymenoptera: Apidae) against pathogenic microorganisms Acta Amazonica
Cruz,Carolinie Batista Nobre da; Pieri,Fabio Alessandro; Carvalho-Zilse,Gislene Almeida; Orlandi,Patrícia Puccinelli; Nunes-Silva,Carlos Gustavo; Leomil,Luciana.
Honeys are described possessing different properties including antimicrobial. Many studies have presented this activity of honeys produced by Apis mellifera bees, however studies including activities of stingless bees honeys are scarce. The aim of this study was to compare the antimicrobial activity of honeys collected in the Amazonas State from Melipona compressipes, Melipona seminigra and Apis mellifera against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Chromobacterium violaceum, and Candida albicans. Minimum inhibitory concentrations were determined using the agar dilution method with Müller-Hinton agar (for bacteria) or Saboraud agar (for yeast). Staphylococcus aureus and E. faecalis were inhibited by all honeys at concentrations...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Melipona; Antibacterial; Antifungal; Natural products; Functional food.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672014000200015
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Antimicrobial activity of bergenin from Endopleura uchi (Huber) Cuatrec Acta Amazonica
Silva,Saulo L. da; Oliveira,Viviane Guedes de; Yano,Tomomasa; Nunomura,Rita de Cássia Saraiva.
Endopleura uchi (Huber) Cuatrec. is an Amazon species traditionally used as treatment for inflammations and female disorders. Bergenin was isolated from ethyl acetate fraction of bark of E. uchi by using column chromatography over sephadex LH-20 and then silica gel 60 flash. Its structure was identified on the basis of its NMR spectra. The antimicrobial activity of bergenin and fractions of methanol extract of E. uchi were evaluated against ATCC microorganisms (Escherichia coli, Salmonella enteritidis, Pseudomonas aeruginosa, Enterococcus faecalis, Staphylococcus aureus, Candida albicans, C. guilliermondii, Aspergillus flavus, A. nidulans). Clinically isolated strains of all of these microorganisms, along with C. tropicalis, A. niger, Shigella sonnei,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibacterial; Antifungal; Candida; Humiriaceae; Uxi-amarelo.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672009000100019
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ANTIFUNGAL ACTIVITY SCREENING OF ANTARCTIC ACTINOBACTERIA AGAINST PHYTOPATHOGENIC FUNGI Acta biol.Colomb.
SANTOS,Andrés; NÚÑEZ-MONTERO,Kattia; LAMILLA,Claudio; PAVEZ,Mónica; QUEZADA-SOLÍS,Damián; BARRIENTOS,Leticia.
ABSTRACT The extreme weather conditions in the Antarctic have exerted selective pressures favoring differential features in bacteria to survive this untapped environment (i.e., antibiotic molecules). Notably, higher chances of antibiotic discovery from extremophiles have been proposed recently. Although new organic and environmentally friendly sources for helping in the control of plant pathogenic fungi are necessary, the information about anti-phytopathogenic applications of extremophile microorganisms from untapped environments is limited. In this study, we determined the antifungal effect of actinobacterial strains isolated from Antarctic soils and sediments. Co-culture inhibition assays and Minimum Inhibitory Concentration (MIC) determination revealed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antagonism; Antarctic bacteria; Antifungal; Extremophile; Plant pathogen.
Ano: 2020 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2020000200353
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Evaluation of in vitro biological properties of Senna occidentalis (L.) Link Biological Sciences
Lombardo, Márcia; Kiyota, Sumika; Kato, Edna Tomiko Miyake; Mathor, Monica Beatriz; Pinto, Terezinha de Jesus Andreoli; Kaneko, Telma Mary.
Senna species have been widely used by American, African and Indian ethic groups mainly in the treatment of feebleness, constipation, liver disorders and skin infections. Senna occidentalis (L.) Link is a perennial shrub native to South America and indigenous to tropical regions throughout the world. Current study evaluated the antimicrobial activity of aqueous and hydroalcoholic extracts from S. occidentalis prepared from different parts of the plant. Antimicrobial activity was assessed against standard pharmaceutical microorganisms by spectrophotometry and microdilution technique. Escherichia coli was sensitive only to compounds extracted from seeds which may be proteinaceous. A broader antimicrobial spectrum was demonstrated by the hydroalcoholic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.10.06.00-8 Cassia occidentalis; Leguminosae; Traditional medicine; Antibacterial; Antifungal; Fibroblasts Etnofarmacologia.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/22525
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Antifungal and antioxidant activity of fatty acid methyl esters from vegetable oils Anais da ABC (AABC)
PINTO,MARIA E.A.; ARAÚJO,STHÉFANE G.; MORAIS,MARCELA I.; SÁ,NÍVEA P.; LIMA,CAROLINE M.; ROSA,CARLOS A.; SIQUEIRA,EZEQUIAS P.; JOHANN,SUSANA; LIMA,LUCIANA A.R.S..
ABSTRACT Fatty acid methyl esters (FAMEs) were obtained from vegetable oils of soybean, corn and sunflower. The current study was focused on evaluating the antifungal activity of FAMEs mainly against Paracoccidioides spp., as well as testing the interaction of these compounds with commercial antifungal drugs and also their antioxidant potential. FAMEs presented small IC50 values (1.86-9.42 μg/mL). All three FAMEs tested showed antifungal activity against isolates of Paracoccidioides spp. with MIC values ranging from 15.6-500 µg/mL. Sunflower FAMEs exhibited antifungal activity that extended also to other genera, with an MIC of 15.6 μg/mL against Candida glabrata and C. krusei and 31.2 μg/mL against C. parapsilosis. FAMEs exhibited a synergetic effect with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antifungal; Antioxidant; Fatty acid methyl esters; Vegetable oils.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000401671
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Antifungal susceptibility of yeasts isolated from anthropogenic watershed Anais da ABC (AABC)
MILANEZI,ANA C.M.; WITUSK,JOÃO P.D.; VAN DER SAND,SUELI T..
Abstract Yeasts are unicellular fungi widespread in the environment, and studies suggest that there is a positive correlation between yeast and polluted aquatic environments. The aim of this study was to analyze the diversity and resistance of yeasts isolated from water samples collected along the Arroio Dilúvio in Porto Alegre to antifungals. Yeast isolates from the Arroio Dilúvio were subjected to susceptibility assays against antifungals using the minimum inhibitory concentration (MIC) test, and amplification of the ITS1-5.8S-IT2 region; in addition, the ITS-5.8S region was sequenced for some of the isolates. The amplification product was subjected to PCR-RFLP, and the restriction profile allowed the construction of a dendrogram. Susceptibility tests...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeast; Antifungal; Watershed; Resistance.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100601
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In vitro cytotoxic, antifungal, trypanocidal and leishmanicidal activities of acetogenins isolated from Annona cornifolia A. St. -Hil. (Annonaceae) Anais da ABC (AABC)
LIMA,LUCIANA A.R.S.; ALVES,TÂNIA M.A.; ZANI,CARLOS L.; SALES JÚNIOR,POLICARPO A.; ROMANHA,ALVARO J.; JOHANN,SUSANA; CISALPINO,PATRÍCIA S.; PIMENTA,LÚCIA P.S.; BOAVENTURA,MARIA AMÉLIA D..
Annona cornifolia A. St. -Hil. is a small annual perennial tree found in the Brazilian savannah; their green fruit is popularly used in the treatment of ulcers. The acetogenins isolated from the seeds of Annona cornifolia previously showed to possess antioxidant activity. In continuation of our investigations on the biological activities of acetogenins, four binary mixtures and ten pure adjacent bis-tetrahydrofuran annonaceous acetogenins were evaluated: the cytotoxic (against three human tumor cell lines), antifungal (against Paracoccidioides brasiliensis), trypanocidal (against Trypanosoma cruzi) and leishmanicidal (against Leishmania amazonensis) activities. Acetogenins presented cytotoxic activity confirming their potential use in anti-cancer therapy....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Annona cornifolia; Acetogenins; Cytotoxic; Antifungal; Trypanocidal; Leishmanicidal.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000200829
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Applications of Plackett–Burman and Central Composite Design for the Optimization of Novel Brevundimonas diminuta KT277492 Chitinase Production, Investigation of its Antifungal Activity BABT
Warda,E. Ashour; Abeer,A. Abd El Aty; Eman,R. Hamed; Mahmoud,A. Swelim; Ahmed,I. El-Diwany.
ABSTRACT Biological control strategy which can damage chitin, a vital component of pathogenic fungi and arthropods promises a safe solution for many fungal problems. And it’s more favorable than chemicals which increase health risks and environmental problems. Thus, the chitinase producers appear potential candidates of biological control of pathogenic fungi. Brevundimonus diminuta KT277492 is a new isolate that has been isolated recently from Egyptian soil. Significant factors that affecting the chitinase enzyme production were studied and optimized using Plackett-Burman and Response Surface Methodology (RSM). As a result, maximum production of chitinase enzyme was 832.87 IUL-1, this result presented about 8.767-fold increase in the enzyme production. In...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitinase; Brevundimonas diminuta KT277492; Optimization; Plackett–Burman; Central composite design; Antifungal.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100429
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Development of modified medium for the enhancement in antifungal activity of P. steckii (MF1 mangrove fungi) against verticillium wilt pathogenic fungi of rose BABT
Sabat,Jyotasanmayee; Gupta,Nibha.
The antifungal activity of Penicillium steckii (MF1) against pathogenic fungi of rose causing verticillium wilt was evaluated under differential nutrient condition. A suitable and modified medium was obtained in which test fungus exhibited enhanced antifungal activity and produced larger inhibition zone against the verticillium fungus. The study carried out to modify the medium components at level of nitrogen, carbon source and their concentration on agar plates. Addition of amino acids, metals and plant growth hormones did not show positive effect but glycerol enhanced the antifungal activity very much.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antifungal; Penicillium; Nutrient; Rose; Verticillium wilt.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400003
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Phytochemical and antifungal activity of anthraquinones and root and leaf extracts of Coccoloba mollis on phytopathogens BABT
Barros,Iuri Bezerra de; Daniel,Juliana Feijó de Souza; Pinto,Jurandir Pereira; Rezende,Maria Inês; Braz Filho,Raimundo; Ferreira,Dalva Trevisan.
The aim of this work was to study the phytochemical and antifungal activity of anthraquinones and root and leaf extracts of Coccoloba mollis on phytopathogens. The chemical analysis of ethanolic extracts showed a mixture of long-chain hydrocarbons, carboxyl esters and 3-taraxerone in the leaf extract. Two anthraquinones (emodin and physcion) were isolated and identified from the root extract. Phytochemical screening using the pharmacognostic methods revealed the presence of flavonoids and tannins in the leaves and roots. Anthraquinones were only found in the root extract, no alkaloids, coumarins, saponins and simple phenolics were present. The antifungal activity of C. mollis extracts and anthraquinones isolated from the root of this plant against...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antifungal; Phytochemical; Lasiodiplodia; Botryosphaeria; Coccoloba mollis.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300015
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In Vitro antifungal potency of plant extracts against five phytopathogens BABT
Tapwal,Ashwani; Nisha,; Garg,Shipra; Gautam,Nandini; Kumar,Rajesh.
The antifungal activity of aqueous extract of Cannabis sativa, Parthenium hysterophorus, Urtica dioeca, Polystichum squarrosum and Adiantum venustum was investigated against Alternaria solani, Alternaria zinniae, Curvularia lunata, Rhizoctonia solani and Fusarium oxysporum at different concentrations (5, 10, 15 and 20%). At 20%, maximum antifungal potential was observed with the extracts of C. sativa, which recorded excellent inhibitory activity against C. lunata (100%), A. zinniae (59.68%), followed by leaf extract of P. hysterophorus (50%) against A. solani. The application of botanical extracts for disease management could be less expensive, easily available, non-polluting and eco-friendly.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Botanical; Phytoextract; Antifungal; Phytopathogen; IPM.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600003
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Fluconazole prophylaxis in preterm infants: a systematic review BJID
Rios,Juliana Ferreira da Silva; Camargos,Paulo Augusto Moreira; Corrêa,Luísa Petri; Romanelli,Roberta Maia de Castro.
ABSTRACT Objective: This article aims to review the use of antifungal prophylaxis with intravenous fluconazole in premature newborns and the occurrence of Invasive Candidiasis. Methods: This is a systematic review with search at databases: PubMed, Capes Portal, Virtual Health Library (BVS - Biblioteca Virtual em Saúde)/Lilacs, Scopus and Cochrane. The keywords used were: "Antifungal", "Candida" "Fluconazole prophylaxis" and "Preterm infants". Results: Invasive Candidiasis was evaluated in all the twelve items. In eleven of them, there was a statistically significant difference between the groups receiving prophylactic fluconazole, with lower frequency of Invasive Candidiasis, compared to placebo or no prophylaxis group. Colonization by Candida species...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antifungal; Candida; Fluconazole prophylaxis; Preterm infants.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000300333
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Acute disseminated paracoccidioidomycosis in a 3 year-old child BJID
Grossklaus,Daciene de Arruda; Tadano,Tomoko; Breder,Sandra Assis; Hahn,Rosane Christine.
Clinical cases involving paracoccidioidomycosis in children, diagnosed in Mato Grosso State, in the central western region of Brazil, are rare despite the state being classified with a moderate to high incidence. We describe a clinical case of infant acute disseminated Paracoccidioidomycosis in Mato Grosso State, Brazil, highlighting the importance of early differential diagnosis from other severe pathologies, thus contributing to the survival of compromised patients. A 3 year-old male child, weighing 12.8 Kg, originating from Vila Rica, MT, Brazil. The patient presented intermittent 40ºC fever evolving over 40 days, dry cough and painless bilateral cervical adenomegaly, showing no signs of inflammation. This was associated with diarrhea, distension and...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Acute paracoccidioidomycosis; Children; Antifungal.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000300018
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Chloroquine sensitizes biofilms of Candida albicans to antifungal azoles BJID
Shinde,Ravikumar Bapurao; Raut,Jayant Shankar; Chauhan,Nitin Mahendra; Karuppayil,Sankunny Mohan.
Biofilms formed by Candida albicans, a human pathogen, are known to be resistant to different antifungal agents. Novel strategies to combat the biofilm associated Candida infections like multiple drug therapy are being explored. In this study, potential of chloroquine to be a partner drug in combination with four antifungal agents, namely fluconazole, voriconazole, amphotericin B, and caspofungin, was explored against biofilms of C. albicans. Activity of various concentrations of chloroquine in combination with a particular antifungal drug was analyzed in a checkerboard format. Growth of biofilm in presence of drugs was analyzed by XTT-assay, in terms of relative metabolic activity compared to that of drug free control. Results obtained by XTT-metabolic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antifungal; Biofilm; Candida albicans; Combination; Chloroquine; Drug resistance.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000400002
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Synergistic action of starch and honey against Candida albicans in correlation with diastase number BJM
Boukraa,Laïd; Benbarek,Hama; Moussa,Ahmed.
To evaluate the synergistic action of starch on the antifungal activity of honey, a comparative method of adding honey with and without starch to culture media was used. Candida albicans has been used to determine the minimum inhibitory concentration (MIC) of five varieties of honey. In a second step, lower concentrations of honey than the MIC were incubated with a set of concentrations of starch added to media to determine the minimum synergistic inhibitory concentration (MSIC). The MIC for the five varieties of honey without starch against C. albicans ranged between 40% and 45% (v/v). When starch was incubated with honey and then added to media, a MIC drop has been noticed with each variety. It ranged between 7% and 25%. A negative correlation has been...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antifungal; Honey; Starch; Diastase Number; Candida albicans.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100010
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Antifungal activity of Cymbopogon winterianus jowitt ex bor against Candida albicans BJM
Oliveira,Wylly Araújo de; Pereira,Fillipe de Oliveira; Luna,Giliara Carol Diniz Gomes de; Lima,Igara Oliveira; Wanderley,Paulo Alves; Lima,Rita Baltazar de; Lima,Edeltrudes de Oliveira.
Candida albicans is an opportunistic yeast and a member of the normal human flora that commonly causes infections in patients with any type of deficiency of the immune system. The essential oils have been tested for antimycotic activity and pose much potential as antifungal agents. This work investigated the activity of the essential oil of Cymbopogon winterianus against C. albicans by MIC, MFC and time-kill methods. The essential oil (EO) was obtained by hydrodistillation using a Clevenger-type apparatus. It was tested fifteen strains of C. albicans. The MIC was determined by the microdilution method and the MFC was determined when an aliquot of the broth microdilution was cultivated in SDA medium. The phytochemical analysis of EO showed presence of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida albicans; Antifungal; Cymbopogon winterianus.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200004
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Efficacy of aqueous garlic extract on growth, aflatoxin B1 production, and Cyto-morphological aberrations of Aspergillus flavus, causing human ophthalmic infection: topical treatment of A. flavus keratitis BJM
Ismaiel,Ahmed A.; Rabie,Gamal H.; Kenawey,Saied E.M.; EL-Aal,Marwa A. Abd.
By using agar well diffusion assay, antifungal activity of aqueous extract prepared from Egyptian garlic (Allium sativum L.) was evaluated in vitro against two strains of Aspergillus flavus (OC1 and OC10) causing human ocular infection. The recorded minimum inhibitory concentration (MIC) for growth inhibition of both strains was 3.60 mg/ml. Aqueous garlic extract (AGE) was used in successive in vivo tests as an attempt to cure rabbit's fungal keratitis caused by A. flavus OC1. Findings showed that diluted preparation of AGE was effective topical antifungal agent and succeeded to cure severe A. flavus keratitis in a time course less than 10 days without any observable side effects. Microscopic examination showed that AGE induced deleterious...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus flavus. Garlic; Antifungal; Keratitis; Microscopic examination; Aflatoxin B1.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400017
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Antifungal activity against Cryptococcus neoformans strains and genotoxicity assessment in human leukocyte cells of Euphorbia tirucalli L. BJM
Oliveira,Luís Flávio Souza de; Fuentefria,Alexandre Meneghello; Klein,Fernanda da Silva; Machado,Michel Mansur.
In the last times, focus on plant research has increased all over the world. Euphorbia tirucalli L., a plant known popularly as Aveloz, and originally used in Africa, has been drawing attention for its use in the United States and Latin America, both for use as an ornamental plant and as a medicinal plant. E. tirucalli L. is a member of the family Euphorbiaceae and contains many diterpenoids and triterpenoids, in particular phorbol esters, apparently the main constituent of this plant, which are assumed to be responsible for their activities in vivo and in vitro. The in vitro antifungal activities of Euphorbia tirucalli (L.) against opportunistic yeasts were studied using microbroth dilution assay. The results showed that aqueous extract and latex...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Euphorbia tirucalli L.; Aveloz; Antifungal; Cryptococcus neoformans.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400027
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Evaluation of antifungal activity in essential oil of the Syzygium aromaticum (L.) by extraction, purification and analysis of its main component eugenol BJM
Rana,Inder Singh; Rana,Aarti Singh; Rajak,Ram Charan.
Antifungal properties of some essential oils have been well documented. Clove oil is reported to have strong antifungal activity against many fungal species. In this study we have evaluated antifungal potential of essential oil of Syzygium aromaticum (L.) against some common fungal pathogens of plants and animals namely, Fusarium moniliforme NCIM 1100, Fusarium oxysporum MTCC 284, Aspergillus sp., Mucor sp., Trichophyton rubrum and Microsporum gypseum. All fungal species were found to be inhibited by the oil when tested through agar well diffusion method. Minimum inhibitory concentration (MIC) was determined for all the species. Column chromatography was performed to separate the eugenol rich fraction from clove oil. Out of seven fractions maximum activity...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Essential oil; Syzygium aromaticum; Antifungal; Chromatography; Eugenol.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400004
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In-vitro antimicrobial activity of Psidium guajava l. leaf extracts against clinically important pathogenic microbial strains BJM
Nair,Rathish; Chanda,Sumitra.
The methanol, acetone and N, N-dimethylformamide (DMF) fractions of leaves of Psidium guajava L. were evaluated for antibacterial and antifungal activity. Piperacillin and gentamicin were used as standards for antibacterial assay, while nystatin and flucanazole were used as standards for antifungal assay. 91 clinically important strains were used for the study which were both clinical isolates as well as identified strains. The antibacterial activity was more pronounced against gram-positive bacterial and fungal strains. Moderate activity was shown against the gram-negative bacterial strains studied.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibacterial; Antifungal; Psidium guajava extracts; Clinical isolates.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300013
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