Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados Biol. Res. (1) Genet. Mol. Biol. (1) Autor Baptista,Francielle (1) Becker,Tânia C.A. (1) CASTRO-PRADO,MARIALBA AVEZUM ALVES DE (1) CHIUCHETTA,SIMONE JUREMA RUGGERI (1) Castro-Prado,Marialba A.A. de (1) Chiuchetta,Simone J.R. (1) Mais... Palavra-chave Antineoplastic drug (2) Aspergillus nidulans (2) Aneuploidy (1) Ethidium bromide (1) Intercalating agent (1) Mitotic crossing over (1) Recombinagenic activity (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2003 (1) 2002 (1) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Increase in mitotic recombination in diploid cells of Aspergillus nidulans in response to ethidium bromide Genet. Mol. Biol. Becker,Tânia C.A.; Chiuchetta,Simone J.R.; Baptista,Francielle; Castro-Prado,Marialba A.A. de. Ethidium bromide (EB) is an intercalating inhibitor of topoisomerase II and its activities are related to chemotherapeutic drugs used in anti-cancer treatments. EB promotes several genotoxic effects in exposed cells by stabilising the DNA-enzyme complex. The recombinagenic potential of EB was evaluated in our in vivo study by the loss of heterozygosity of nutritional markers in diploid Aspergillus nidulans cells through Homozygotization Index (HI). A DNA repair mutation, uvsZ and a chromosome duplication DP (II-I) were introduced in the genome of tested cells to obtain a sensitive system for the recombinagenesis detection. EB-treated diploid cells had HI values significantly greater than the control at both concentrations (4.0 x 10-3 and 5.0 x 10-3 mM).... Tipo: Info:eu-repo/semantics/article Palavras-chave: Antineoplastic drug; Aspergillus nidulans; Ethidium bromide; Intercalating agent; Mitotic crossing over. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300026 Vincristine induces somatic segregation, via mitotic crossing-over, in diploid cells of Aspergillus nidulans Biol. Res. CHIUCHETTA,SIMONE JUREMA RUGGERI; CASTRO-PRADO,MARIALBA AVEZUM ALVES DE. Vincristine is an alkaloid widely used as an antineoplastic agent. In eukaryotic cells the drug causes blockage in the G2 phase of the cell cycle and an increase in the frequency of sister chromatid exchanges. Due to the fact that germinating Aspergillus nidulans cells spend most of their cycle in G2 phase, they provide an excellent system for the study of mitotic crossing-over. Taking into account that mitotic crossing-over occurs during G2 period, the evaluation of recombinagenic and aneugenic potential of vincristine is provided with regard to two diploid strains of A. nidulans: a wild strain (uvsH+//uvsH+) and a defective one in DNA repair (uvsH//uvsH). Drug toxicity and its effect on the asexual cycle of A. nidulans has been evaluated as well.... Tipo: Journal article Palavras-chave: Aneuploidy; Antineoplastic drug; Aspergillus nidulans; Recombinagenic activity. Ano: 2002 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100006 Registros recuperados: 2 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco