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Typical and atypical enteropathogenic Escherichia coli bacterial translocation associated with tissue hypoperfusion in rats 56
Liberatore,A.M.A.; Moreira,F.C.; Gomes,T.A.T.; Menchaca-Diaz,J.L.; Koh,I.H.J..
Although enteropathogenic Escherichia coli (EPEC) are well-recognized diarrheal agents, their ability to translocate and cause extraintestinal alterations is not known. We investigated whether a typical EPEC (tEPEC) and an atypical EPEC (aEPEC) strain translocate and cause microcirculation injury under conditions of intestinal bacterial overgrowth. Bacterial translocation (BT) was induced in female Wistar-EPM rats (200-250 g) by oroduodenal catheterization and inoculation of 10 mL 10(10) colony forming unit (CFU)/mL, with the bacteria being confined between the duodenum and ileum with ligatures. After 2 h, mesenteric lymph nodes (MLN), liver and spleen were cultured for translocated bacteria and BT-related microcirculation changes were monitored in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enteropathogenic Escherichia coli (EPEC); Atypical EPEC; Bacterial translocation; Microcirculation; Tissue perfusion.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000007
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Captive wild birds as reservoirs of enteropathogenic E. coli (EPEC) and Shiga-toxin producing E. coli (STEC) 58
Sanches,Lilian Aparecida; Gomes,Marcelo da Silva; Teixeira,Rodrigo Hidalgo Friciello; Cunha,Marcos Paulo Vieira; Oliveira,Maria Gabriela Xavier de; Vieira,Mônica Aparecida Midolli; Gomes,Tânia Aparecida Tardelli; Knobl,Terezinha.
ABSTRACT Psittacine birds have been identified as reservoirs of diarrheagenic Escherichia coli, a subset of pathogens associated with mortality of children in tropical countries. The role of other orders of birds as source of infection is unclear. The aim of this study was to perform the molecular diagnosis of infection with diarrheagenic E. coli in 10 different orders of captive wild birds in the state of São Paulo, Brazil. Fecal samples were analyzed from 516 birds belonging to 10 orders: Accipitriformes, Anseriformes, Columbiformes, Falconiformes, Galliformes, Passeriformes, Pelecaniformes, Piciformes, Psittaciformes and Strigiformes. After isolation, 401 E. coli strains were subjected to multiplex PCR system with amplification of genes eae and bfp...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diarrheagenic E. coli; Atypical EPEC; STEC; Avian diseases.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400760
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