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Metabolic networks and bioenergetics of Aurantiochytrium sp. B-072 during storage lipid formation BJM
Chaisawang,Montri; Verduyn,Cornelis; Chauvatcharin,Somchai; Suphantharika,Manop.
Baffled shake flask cultivation of Aurantiochytrium sp. B-072 was carried out at in a glucose-monosodium glutamate mineral medium at different C/N-ratios (30-165) with glucose fixed at 90 g/L. With increasing C/N-ratio, a modest increase in lipid content (60 to 73 % w/w) was observed whereas fat-free biomass decreased but overall biomass showed little variation. FA-profiles were not affected to a large extent by C/N-ratio and absolute docosahexaenoic (DHA)-levels fell in narrow range (5-6 g/L). However at C/N > 64 a rapid decrease in lipid synthetic rate and/or incomplete glucose utilization occurred. Glucose and FA-fluxes based on fat-free biomass peaked at a C/N ratio of 56. This condition was chosen for calculation of the redox balance (NAD(P)H) and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aurantiochytrium; C/N-ratio; Docosahexaenoic acid; Fatty acid flux; P/O ratio.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300047
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