|
|
|
|
|
| Onishi, Kenji; Sarumoh, Bolu Muhammad; Hagiwara, Daisuke; Watanabe, Akira; Kamei, Katsuhiko; Toyotome, Takahito. |
| Azole-resistant strains of Aspergillus fumigatus containing a tandem repeat in the cyp51A promoter and amino acid substitution(s) have been isolated in the environment worldwide; however, this type of resistant strain had never been isolated from the environment in Japan. Our previous study indicated that an azole-resistant A. fumigatus strain OKH50 containing a 34-bp tandem repeat in cyp51A promoter with L98H substitution in Cyp51A was isolated from a patient in Obihiro of Hokkaido, Japan. In this study, we collected azole-resistant Aspergillus spp. by air sampling from the environment in Japan. One Aspergillus-like colony was isolated from one of 10 sampling sites surveyed. The strain Env1 was confirmed as A. fumigatus by nucleotide sequencing and... |
|
Palavras-chave: Aspergillus fumigatus; Azole; Cyp51A; TR34/L98H. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4565 |
| |
|
|
| Toyotome, Takahito; Fujiwara, Takayuki; Kida, Hideyuki; Matsumoto, Munehiro; Wada, Terumi; Komatsu, Ryoichi. |
| Azole antifungals are used not only clinically for fungal infections but also used as agricultural fungicides. Recently, azole-resistant Aspergillus fumigatus containing a tandem repeat in the promoter region of cyp51A combined with amino acid substitution(s) appears in the environment in Eurasia, especially in several European countries. Although azole fungicides have been used in Japan, especially in Hokkaido, surveillance and characterization of A. fumigatus in Hokkaido have not been reported. In this study, we collected soil samples from farms that used an azole fungicide in the Tokachi area of eastern Hokkaido, isolated 91 A. fumigatus strains, and determined the minimal inhibitory concentrations of medical azoles required for these strains. Moreover,... |
|
Palavras-chave: Azole; Aspergillus fumigatus; Cyp51A. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4469 |
| |
|
|
|
