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| Sivakumar, Thillaiampalam; Igarashi, Ikuo; Yokoyama, Naoaki. |
| Babesia ovata, which is transmitted by Haemaphysalis longicornis, is an intraerythrocytic protozoan parasite of cattle. Based on its morphology, B. ovata is classified as a large-type Babesia. The developmental stages of B. ovata have been described both in cattle and the tick vector. In infected adult female ticks, the parasite is transovarially transmitted to the tick eggs. The sexual reproduction of B. ovata has been demonstrated in the tick midgut. The diagnostic tools that are currently available for the specific detection of B. ovata in cattle include microscopy and polymerase chain reaction assays. The development of improved molecular and serological diagnostic tools has been constrained by the limited availability of genetic data. B. ovata has... |
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Palavras-chave: Babesia ovata; Cattle; Epidemiology; Phylogeny; Taxonomy. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4555 |
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| SIVAKUMAR, Thillaiampalam; ASADA, Masahito; BATTSETSEG, Badgar; HUANG, Xiaohong; LAN, Dinh Thi Bich; INPANKAEW, Tawin; P. YBAÑEZ, Adrian; ALHASSAN, Andy; THEKISOE, Oriel M. M.; DE MACEDO, Alan Caine Costa; INOKUMA, Hisashi; IGARASHI, Ikuo; YOKOYAMA, Naoaki; 猪熊, 壽; 五十嵐, 郁男; 横山, 直明. |
| Babesia ovata is a tick-transmitted hemoprotozoan parasite that infects cattle. In our study, bovine blood samples (n=2,034) were collected from 10 different countries (Brazil, China, Ghana, Japan, Mongolia, the Philippines, South Africa, Sri Lanka, Thailand and Vietnam) and DNA extracted. The DNA samples were screened using an established and specific polymerase chain reaction (PCR) assay targeting the Apical membrane antigen 1 (AMA-1) gene. Parasite DNA was detected among samples collected from Japan, Mongolia and Thailand. Sequence analyses confirmed that the PCR assay detected only B. ovata AMA-1, and that amplicons from different geographical locations were conserved. Our findings highlight the importance of designing adequate strategies to control B.... |
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Palavras-chave: AMA-1; Babesia ovata; Cattle; Mongolia; Thailand. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4016 |
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