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Registros recuperados: 11 | |
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Bogas,Andréa C.; Watanabe,Maria Angelica E.; Barbosa,Aneli; Vilas-Boas,Laurival A.; Bonatto,Ana C.; Dekker,Robert; Souza,Emanuel M.; Fungaro,Maria Helena P.. |
A beta-glucosidase-like enzyme-encoding gene (bglH) of an endophytic Bacillus pumilus strain (CL16) was cloned using a shotgun genomic library constructed in Escherichia coli. The nucleotide sequence of the entire cloned fragment (2484 bp) was determined and characterized. An incomplete open reading frame (ORF) of 534 bp (ORF1) designated bglP and a complete ORF of 1419 bp (ORF2) designated bglH, located in the fragment, are organized in an operon. The protein deduced from 1419 bp (ORF2) had 472 amino acid residues without a characteristic signal peptide sequence, suggesting that the enzyme is localized in the cytoplasm. The amino acid sequence deduced from bglH gene had high similarity with beta-glucosidases from the glycosyl hydrolase family 1.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus pumilus; BglH; Glucosidase; Glycosyl hydrolase 1; PTS. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100018 |
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Silva,Fabíola J; Ribeiro,Regina CF; Xavier,Adelica A; Santos Neto,José A; Souza,Maione A; Dias-Arieira,Cláudia R. |
RESUMO: Agentes de controle biológico associados com matéria orgânica é uma estratégia que pode potencializar o controle dos nematoides-das-galhas. Objetivou-se neste trabalho avaliar o efeito de materiais orgânicos e rizobactérias sobre Meloidogyne javanica em tomateiro. Utilizou-se o delineamento em blocos casualizados, em esquema fatorial 4x2+1, sendo quatro materiais orgânicos (turfa, biofertilizante, esterco bovino e composto orgânico de casca de pequi), dois isolados de rizobactérias (Bacillus pumilus -1 e B. pumilus - 76) e adubação mineral (tratamento adicional), com oito repetições. Mudas de tomateiro foram transplantadas para vasos contendo solo arenoso, ao qual incorporaram-se os diferentes materiais orgânicos. As suspensões das rizobactérias... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lycopersicon esculentum; Meloidogyne javanica; Controle biológico; Bacillus pumilus; Matéria orgânica.. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362016000100059 |
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Gomes,Andréa M.A.; Mariano,Rosa L.R.; Silveira,Elineide B.; Mesquita,Júlio C.P.. |
Isolados bacterianos epifíticos e endofíticos, obtidos de plantas sadias de alface, foram avaliados para promoção de crescimento de mudas e plantas, respectivamente em estufa e campo de cultivo orgânico (Chã Grande-PE). Nos experimentos em estufa, foi utilizada a cultivar Verônica e em campo, as cultivares Verdinha e Verônica. Os isolados foram aplicados por bacterização simultânea nas sementes e substrato. Em campo, foram utilizados os isolados mais eficientes, C25 (Bacillus thuringiensis subvar. kenyae) e C116 (Bacillus pumilus), separadamente e em mistura, após teste de compatibilidade. Em estufa, foram avaliadas a matéria fresca de raízes (MFR), da parte aérea (MFPA) e total (MFT), 21 dias após a bacterização. Em campo, foi determinado o peso da... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lactuca sativa; Bacillus pumilus; Bacillus thuringiensis subvar; Kenyae; Bactérias promotoras de crescimento de plantas; Mecanismos de ação; Sistema orgânico. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362003000400026 |
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Parvathi,Ammini; Krishna,Kiran; Jose,Jiya; Joseph,Neetha; Nair,Santha. |
Bacillus species constitute a diverse group of bacteria widely distributed in soil and the aquatic environment. In this study, Bacillus strains isolated from the coastal environment of Cochin, India were identified by detailed conventional biochemical methods, fatty acid methyl ester (FAME) analysis and partial 16S rDNA sequencing. Analysis of the data revealed that Bacillus pumilus was the most predominant species in the region under study followed by B. cereus and B. sphaericus. The B. pumilus isolates were further characterized by arbitrarily primed PCR (AP-PCR), antibiotic sensitivity profiling and PCR screening for known toxin genes associated with Bacillus spp. All B. pumilus isolates were biochemically identical, exhibited high protease and lipase... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus pumilus; FAME; 16S rDNA; CesB; AP-PCR. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200012 |
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Oliveira,Patrícia Lopes de; Duarte,Marta Cristina Teixeira; Ponezi,Alexandre Nunes; Durrant,Lúcia Regina. |
The production of manganese peroxidase (MnP) from Bacillus pumilus and Paenibacillus sp. was studied under absence and presence of the inducers indulin AT, guayacol, veratryl alcohol, lignosulfonic acid and lignosulfonic acid desulfonated. Indulin AT increased the activity of B. pumilus MnP up to 31.66 U/L after 8 h, but no improve was observed for Paenibacillus sp., which reached maximum activity (12.22 U/L) after 20 h. Both MnPs produced by these microorganisms were purified in phenyl sepharose resin and the proteins from crude extracts were eluted in two fractions. However, only the first fraction of each extract exhibited MnP activities. Tests in different pH and temperature values, from pH 5.0 to pH 10.0 and 30 ºC to 60 ºC, respectively, were carried... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus pumilus; Paenibacillus sp.; Manganese peroxidase; Purification; Characterization. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400012 |
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Sangeetha,R.; Arulpandi,I.; Geetha,A.. |
Proteolysis-resistant lipases can be well exploited by industrial processes which employ both lipase and protease as biocatalysts. A proteolysis resistant lipase from Bacillus pumilus SG2 was isolated, purified and characterized earlier. The lipase was resistant to native and commercial proteases. In the present work, we have characterized the lip gene which encodes the proteolysis-resistant lipase from Bacillus pumilus SG2. The parameters and structural details of lipase were analysed. The lip gene consisted of 650 bp. The experimental molecular weight of SG2 lipase was nearly double that of its theoretical molecular weight, thus suggesting the existence of the functional lipase as a covalent dimer. The proteolytic cleavage sites of the lipase would have... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus pumilus; Covalent dimer; Lipase; Proteolytic-resistance. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200004 |
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Registros recuperados: 11 | |
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