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Tiba,Monique Ribeiro; Moura,Claúdia de; Carazzolle,Marcelo Falsarella; Leite,Domingos da Silva. |
Escherichia coli has been isolated frequently, showing flagellar antigens that are not recognized by any of the 53 antisera, provided by the most important reference center of E. coli, The International Escherichia and Klebsiella Center (WHO) of the Statens Serum Institute, Copenhagen, Denmark. The objective of this study was to characterize flagellar antigens of E. coli that express non-typeable H antigens. The methods used were serology, PCR-RFLP and DNA sequencing. This characterization was performed by gene amplification of the fliC (flagellin protein) by polymerase chain reaction in all 53 standards E.coli strains for the H antigens and 20 E. coli strains for which the H antigen was untypeable. The amplicons were digested by restriction enzymes, and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Antigens; Bacterial; Polymerase chain reaction; Polymorphism; Restriction fragment length. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200009 |
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Kiffer,Carlos; Hsiung,Andre; Oplustil,Carmen; Sampaio,Jorge; Sakagami,Elsa; Turner,Philip; Mendes,Caio. |
Establish the susceptibility pattern of Gram-negative bacteria causing infections in ICU patients, MYSTIC Program Brazil 2003. Gram-negative bacteria (n = 1,550) causing nosocomial infections were collected at 20 Brazilian centers. The central laboratory confirmed the identification and performed the susceptibility tests by Etest methodology (AB Biodisk, Solna, Sweden) for meropenem, imipenem, ciprofloxacin, ceftazidime, cefepime, cefotaxime, piperacillin/tazobactam, gentamicin, and tobramycin. Interpretation criteria used were according to National Committee for Clinical Laboratory Standards (NCCLS). Pseudomonas aeruginosa (30.3%) was the most frequent isolate, followed by E. coli (18.6%), Klebsiella pneumoniae (16.9%), Acitenobacter baumannii (8.8%), and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Drug resistance; Bacterial; Microbial sensitivity tests; Infection control; Carbapenems. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000300004 |
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Villegas,Maria Virginia; Briceno,David Felipe; Ruiz,Sory Jamil; Furtado,Guilherme H; Nicolau,David P. |
OBJECTIVES: This study was designed to simulate standard and optimized dosing regimens for intravenous antibiotics against contemporary populations of Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, and Pseudomonas aeruginosa using MIC distribution data to determine which of the tested carbapenem regimens provided the greatest opportunity for obtaining maximal pharmacodynamic (PD) activity. METHODS: The isolates studied were obtained from the COMPACT-COLOMBIA surveillance program conducted between February and November 2009. Antimicrobial susceptibility testing was conducted by broth microdilution method according to the CLSI guidelines. Doripenem, imipenem-cilastatin, and meropenem, were the modeled antibiotics. A 5,000 patient Monte... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Drug resistance; Bacterial; Gram-negative bacteria; Monte Carlo Method; Colombia; Pharmacology. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000500001 |
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Mendes,Caio; Oplustil,Carmen; Sakagami,Elsa; Turner,Philip; Kiffer,Carlos. |
OBJECTIVE: Establish the susceptibility pattern of Gram-negative bacteria causing infections in ICU patients, MYSTIC Program Brazil 2002. MATERIAL AND METHODS: Gram-negative bacteria (n = 503) causing nosocomial infections were collected at seven Brazilian centers. The central laboratory confirmed the identification and performed the susceptibility tests by E-test methodology (AB Biodisk, Solna, Sweden) for meropenem, imipenem, ciprofloxacin, ceftazidime, cefepime, cefotaxime, piperacillin/tazobactam, gentamicin, and tobramycin. Interpretation criteria used were according to National Committee for Clinical Laboratory Standards (NCCLS). RESULTS: Pseudomonas aeruginosa (33%) was the most frequently isolated, followed by A. baumannii (17.1%), K. pneumoniae... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Drug resistance; Bacterial; Microbial sensitivity tests; Infection control; Carbapenems. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000100008 |
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Araujo,Orlei Ribeiro de; Silva,Dafne Cardoso Bourguignon da; Diegues,Ana Regina; Arkader,Ronaldo; Cabral,Eloíza Aparecida Ferreira; Afonso,Marta Rodriguez; Louzada,Maria Eduarda; Albertoni,Andréa de Cássia Stéfano. |
Antibiotic restriction can be useful in maintaining bacterial susceptibility. The objective of this study was verify if restriction of cefepime, the most frequently used cephalosporin in our neonatal intensive care unit (NICU), would ameliorate broad-spectrum susceptibility of Gram-negative isolates. Nine hundred and ninety-five premature and term newborns were divided into 3 cohorts, according to the prevalence of cefepime use in the unit: Group 1 (n=396) comprised patients admitted from January 2002 to December 2003, period in which cefepime was the most used broad-spectrum antibiotic. Patients in Group 2 (n=349) were admitted when piperacillin/tazobactam replaced cefepime (January to December 2004) and in Group 3 (n=250) when cefepime was reintroduced... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Drug resistance; Infection control; Cefepime; Bacterial. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000200022 |
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Cury,Eunice Stella Jardim; Chang,Marilene Rodrigues; Pontes,Elenir Rose Jardim Cury. |
Abstract This study compares patients with and without non-viral microbial keratitis in relation to sociodemographic variables, clinical aspects, and involved causative agent. Clinical aspects, etiology and therapeutic procedures were assessed in patients with and without keratitis that were diagnosed in an Eye Care Center in Campo Grande, MS, Brazil. Patients were divided into two groups: (a) cases: 64 patients with non-viral microbial keratitis diagnosed at biomicroscopy; and (b) controls: 47 patients with other eye disorders that were not keratitis. Labor activity related to agriculture, cattle raising, and contact lens use were all linked to keratitis occurrence (p < 0.005). In patients with keratitis, the most common symptoms were pain and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Keratitis; Cornea ulcer; Eye Infections; Bacterial; Eye Infections; Fungal; Acanthamoeba keratitis. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500205 |
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Le Groumellec, M.; Haffner, Philippe; Martin, B.; Martin, C.. |
Mass mortality associated with luminescence has been dbserved in sorne Thai and Ecuadorian penaeid shrimp hatcheries in recent years. Samples collected from hatcheries in these two different areas revealed luminescent sucrose-negative Vibrio strains in larVal rearing tanks where mortality occurred. Pathogenicity trials were carried out to identify the causative agents of mortality and to study the course of infection . . Results showed that strains isolated from Thai hatclleries were more virulent than Ecuadorian strains when tested on different larval stages of Penaeus monodon. The virulence of these strains was also estimated. Trials on other species ofpenaeid shrimp demonstrated the specifie action of Thai strains. Drug sensitivity patterns revealed a... |
Tipo: Text |
Palavras-chave: Mass mortality; Bacterial; Vibrio; Hatcheries; Penaeus monodon; Shrimp. |
Ano: 1993 |
URL: http://archimer.ifremer.fr/doc/1993/acte-6100.PDF |
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El-Shenawy, M.; Farag, A.. |
Spatial and temporal variability in the saprophytic (SB) and salt tolerant saprophytic (STSB) bacterial counts as well as total coliforms (TC), E. coli (EC) and fecal streptococci (FS) were investigated in the Egyptian surface coastal waters of Aqaba and Suez Gulfs as well as Red Sea. A total of 200 samples collected from 40 sites during January, March, May, July and September 2002 were investigated. Hydrographical parameters including water temperature (oC), salinity (S%o), dissolved oxygen (DO) and pH were also measured. According to the European and Egyptian current standards, out of 200 samples analyzed during 2002, 183 samples (91.5%) were accepted for marine recreational purposes. The obtained results indicated that water quality of the investigated... |
Tipo: Journal Contribution |
Palavras-chave: Recreational waters; Water quality; Bacterial; Indicators; Saprophytic bacteria Bacteria Coastal waters Bacteria http://aims.fao.org/aos/agrovoc/c_765. |
Ano: 2005 |
URL: http://hdl.handle.net/1834/1255 |
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