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Establishment of an in vitro system for studies on the induced resistance of cotton to Xanthomonas campestris pv. malvacearum PAB
KOBAYASHI,ADILSON KENJI; VIEIRA,LUIZ GONZAGA ESTEVES.
An in vitro system for studying the resistance response of cotton (Gossypium hirsutum L.) to Xanthomonas campestris pv. malvacearum was investigated. Cell suspension cultures, established from hypocotyl-derived callus of cotton cultivar 101-102B, were treated with bacterial extracellular polysaccharides (EPS) extracted from the incompatible race 18 of X. campestris pv. malvacearum. EPS at 600 mug/mL caused pronounced darkening of the suspension cultures, as indicative of cell death, 48 hours after incubation. Protein electrophoresis analysis of the time course of EPS-treated cells showed differential accumulation of several protein bands after 12-24 hours. The time course of protein accumulation and cell death was consistent with an elicitor-mediated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gossypium hirsutum; Bacterial blight; Extracellular polysaccharides; Cell suspension culture; Hypersensitive response.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2000000400007
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การใช้เครื่องหมายโมเลกุลชนิด STSs สำรวจแหล่งพันธุกรรมยีนต้านทาน Thai Agricultural
Payorm Cobelli; Jittima Wongnongwa; Varapong Chamarerk; Somsong Chotechuen; Rasamee Dhitikiattipong; Witchuda Rattanakarn; Nuchakarn Sinprasit; Teerada Wangsomboondee.
Bacterial blight (BB) disease of rice, caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most serious diseases in all major rice-growing areas of Thailand. The most effective approach to control BB is the use of resistant varieties. However, the selection pressure from the usage of resistant cultivars may result in mutation of the pathogen population to overcome the resistance genes. Therefore, using genetic resources conferring different resistance genes are more advantage in rice improvement. The aim of this research was to survey and screen BB resistance genes in Thai local rice varieties from the National Rice Seed Storage Laboratory for Genetic Resources to gain more genetic diversity for resistance. In this study, the Thai Local rice...
Tipo: PhysicalObject Palavras-chave: Bacterial blight; Molecular marker; BB resistance genes; BB greenhouse screening; โรคขอบใบแห้ง; เครื่องหมายโมเลกุล; ยีนต้านทานโรคขอบใบแห้ง; การคัดเลือก; สภาพเรือนทดลอง; ความต้านทานต่อโรค.
Ano: 2014 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5652
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การใช้ประโยชน์เครื่องหมายโมเลกุลชนิด STS สำหรับงานปรับปรุงพันธุ์ข้าวไทย ให้ต้านทานต่อโรคขอบใบแห้งแบบคงทนถาวร Thai Agricultural
Payorm Cobelli; Somjai Saleeto; Teerada Wangsomboondee; Anuchart Kotchasatit; Jittima Wongnongwa; Varapong Chamarerk; Rasamee Dhitikiattipong; Witchuda Rattanakarn; Nutchakarn Sinprasit; Pornparn Theerattha; Sirada Oncharoen.
Bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most serious diseases of rice in all major rice-growing areas of Thailand. The most effective approach to control BB is the use of resistant varieties which is economically feasible, an environmental-friendly control method and easy for farmers to handle. However, the selection pressure from the usage of resistant cultivars may result in mutation of the pathogen population to overcome the resistance genes. A feasible method to solve this problem is by pyramiding multiple resistance genes, which are board spectrum, into susceptible rice varieties. The aims of this research were to identify the effectiveness of board spectrum BB resistance genes and to better understand the...
Tipo: Collection Palavras-chave: Bacterial blight; Resistance gene; Molecular marker; Board spectrum resistance; Durable resistance; โรคขอบใบแห้ง; ยีนต้านทานโรคขอบใบแห้ง; เครื่องหมายโมเลกุลชนิด STS; ต้านทานแบบกว้างขวาง; ต้านทานแบบคงทนถาวร.
Ano: 2015 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5857
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การใช้ประโยชน์เครื่องหมายโมเลกุลชนิด STS สำหรับงานปรับปรุงพันธุ์ข้าวไทยให้ต้านทานต่อโรคขอบใบแห้งแบบคงทนถาวร Thai Agricultural
Payorm Cobelli; Somjai Saleeto; Teerada Wangsomboondee; Anuchart Kotchasatit; Jittima Wongnongwa; Varapong Chamarerk; Rasamee Dhitikiattipong; Witchuda Rattanakarn; Nutchakarn Sinprasit; Pornparn Theerattha; Sirada Oncharoen.
Bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most serious diseases of rice in all major rice-growing areas of Thailand. The most effective approach to control BB is the use of resistant varieties which is economically feasible, an environmental-friendly control method and easy for farmers to handle. However, the selection pressure from the usage of resistant cultivars may result in mutation of the pathogen population to overcome the resistance genes. A feasible method to solve this problem is by pyramiding the effective and board spectrum of multiple resistance genes into susceptible rice varieties. The aim of this research was to identify the effectiveness and board spectrum of BB resistance genes and better...
Tipo: Collection Palavras-chave: Bacterial blight; Bacterial blight resistance gene; STS molecular marker; Board spectrum resistance; Durable resistance; โรคขอบใบแห้ง; ยีนต้านทานโรคขอบใบแห้ง; เครื่องหมายโมเลกุลชนิด STS; ต้านทานแบบกว้างขวาง; ต้านทานแบบคงทนถาวร.
Ano: 2016 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5950
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Cloning of a peroxidase gene from cassava with potential as a molecular marker for resistance to bacterial blight BABT
Pereira,Luiz Filipe; Goodwin,Paul H.; Erickson,Larry.
Cassava bacterial blight (CBB), caused by Xanthomonas axonopodis pv. manihotis, is considered one of the most important bacterial diseases of cassava (Manihot esculenta Crantz). In order to characterize the cassava genes involved in resistance to this disease, a genomic clone of a cationic peroxidase gene, MEPX1, was isolated by PCR from cassava cultivar MCOL 22. The DNA sequence of MEPX1 showed high homology with other plant peroxidase genes and contained a large intron typical of peroxidase genes. The predicted translation product showed a heme-ligand motif, also a characteristic of peroxidases, as well as phosphorylation, myristoylation and glycosylation sites. The amino acid sequence had 75 % homology with two Arabidopsis thaliana peroxidases. A...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Manihot esculenta; Peroxidase; Bacterial blight; Xanthomonas axonopodis.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200002
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Green propolis ethanolic extract in bean plant protection against bacterial diseases Ciência Rural
Jaski,Jonas Marcelo; Telaxka,Fabio Junior; Moura,Gabriela Silva; Franzener,Gilmar.
ABSTRACT: The aim of this study was to evaluate the ethanolic extract of green propolis (EEP) in the protection of common bean plants against two main bacterial cultures, bacterial blight (Xanthomonas axonopodis pv. phaseoli) and wildfire (Pseudomonas syringae pv. tabaci). Experiments on antimicrobial activity were performed, inducing phytoalexins, defense-related enzymes, and disease severity, under concentrations of 0, 0.5, 1.0, 2.5, and 5.0%. The EEP presented antimicrobial activity on both phytobacteria, causing a decrease in their development. It has also promoted a linear accumulation of phaseolin in bean hypocotyls according to the EEP concentration used. There was a reduction in the lesion area, which was caused by bacterial blight on bean leaves...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alternative control; Wildfire; Bacterial blight; Resistance induction.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000600151
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