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Nephrotoxicity of Bence-Jones proteins: correlation with endocytosis by BHK cells and intracellular movement BABT
Nicastri,Ana Lucia; Gomes,Elisa Maines; Prado,Maria José Brandão de Almeida; Prado,Euthymia Brandão de Almeida.
The aim of this investigation was to evaluate the endocytosis of two Bence-Jones proteins by renal cells in order to elucidate the interference of their physical and chemical characteristics on nephrotoxicity. Bence-Jones proteins (AK and GL) were purified and isolated from the urine of two patients with multiple myeloma. The isotype of both proteins was characterised as being human monoclonal lambda light chain. The AK protein presented mainly an Ip>7.0, a high content of galactose and a low amount of sialic acid molecules. On the other hand, the GL protein presented a single band with an Ip of 4.3, a higher level of sialic acid and a reduced amount of galactose, in comparison with the AK protein. Baby Hamster Kidney (BHK) cells were maintained in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endocytosis; Bence-Jones; Experimental Nephrology.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200009
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