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Investigations into the uptake of copper, iron and selenium by a highly sulphated bacterial exopolysaccharide isolated from microbial mats ArchiMer
Moppert, Xavier; Le Costaouec, Tinaig; Raguenes, Gerard; Courtois, Anthony; Simon-colin, Christelle; Crassous, Philippe; Costa, Bernard; Guezennec, Jean.
A bacterium isolated from microbial mats located on a polynesian atoll produced a high molecular weight (3,000 kDa) and highly sulphated exopolysaccharide. Previous studies showed that the chemical structure of this EPS consisted of neutral sugars, uronic acids, and high proportions of acetate and sulphate groups. The copper- and iron-binding ability of the purified pre-treated native EPS was investigated. Results showed that this EPS had a very high affinity for both copper (9.84 mmol g(-1) EPS) and ferrous iron (6.9 mmol g(-1) EPS). Amazingly, this EPS did not show any affinity for either ferric ions or selenium salts. This finding is one of the first steps in assessing the biotechnological potential of this polysaccharide.
Tipo: Text Palavras-chave: Sulphated exopolysaccharide; Microbial mats; Iron; Copper; Binding capacity.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6397.pdf
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In vitro ability of beer fermentation residue and yeast-based products to bind aflatoxin B1 BJM
Bovo,Fernanda; Franco,Larissa Tuanny; Rosim,Roice Eliana; Barbalho,Ricardo; Oliveira,Carlos Augusto Fernandes de.
This study aimed to verify the in vitro ability of beer fermentation residue (BFR) containing Saccharomyces cerevisiae cells and five commercial products that differed in the viability and integrity of S. cerevisiae cells to remove aflatoxin B1 (AFB1) from a citrate-phosphate buffer solution (CPBS). BFR was collected at a microbrewery and prepared by drying and milling. The commercial yeast-based products were as follows: inactive intact yeast cells from beer alcoholic fermentation, inactive intact yeast cells from sugarcane alcoholic fermentation, hydrolyzed yeast cells, yeast cell walls and active yeast cells. Adsorption assays were performed in CPBS spiked with 1.0 μg AFB1/mL at pH 3.0 and 6.0 for a contact time of 60 min at room temperature. Analysis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AFB1; Binding capacity; Decontamination; S. cerevisiae cells.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200577
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