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Enhancement of botrallin and TMC-264 production in liquid culture of endophytic fungus Hyalodendriella sp. Ponipodef12 after treatments with metal ions Electron. J. Biotechnol.
Luo,Haiyu; Xu,Dan; Xie,Rushan; Zhang,Xuping; Wang,Jian; Xuejiao,Dong; Lai,Daowan; Zhou,Ligang; Liu,Yang.
Background: Hyalodendriella sp. Ponipodef12, an endophytic fungus from a poplar hybrid, was a high producer of botrallin and TMC-264 with various bioactivities. In this study, the influences of eight metal ions (i.e.,Mn2+,Na+, Mg2+,Zn2+,Cu2+,Fe2+,Fe3+ and Al3+) on botrallin and TMC-264 production in liquid culture of the endophytic fungus Hyalodendriella sp. Ponipodef12 were investigated. Results: Three most effective metal ions (Zn2+,Cu2+ and Mg2+) along with their optimum concentrations were screened. The optimum addition time and concentrations of Zn2+,Cu2+ and Mg2+ were further obtained respectively for improving botrallin and TMC-264 production. The combination effects of Zn2+,Cu2+ and Mg2+ on the production of botrallin and TMC-264 by employing...
Tipo: Journal article Palavras-chave: Combination effects; Production; Microbial biotechnology; Process biotechnology; Bioactivities; Central composite design; Response surface methodology.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600002
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Inhibition of coagulation proteases and thrombosis and sub-chronic toxicological study of a sulfated polysaccharidic fraction from the red alga Gelidiella acerosa Biological Sciences
Queiroz, Ismael Nilo Lino de; Rodrigues, José Ariévilo Gurgel; Quinderé, Ana Luíza Gomes; Holanda, Márjory Lima; Pereira, Maria Gonçalves; Benevides, Norma Maria Barros.
Metabolites isolated from Gelidiella species (Rhodophyta) have been few studied. We evaluated a sulfated polysaccharidic fraction from G. acerosa collected from two Brazilian beaches on the northwestern coast of Brazil (Flecheiras-F and Pedra Rachada-PR) on coagulation proteases and thrombosis. Their toxicity in vivo was also assessed. Enzymatic extractions yielded 1.40%, and similar chromatographic profiles (DEAE-cellulose) were obtained, with fractions (Ga-I→V) containing differences among the relative proportions of sulfate (5-42%), and revealing charge density patterns by electrophoresis. Ga-IV-PR had a discrete effect (3.01 IU mg-1) on normal human coagulation compared with heparin (193 IU mg-1) and was tested on coagulation proteases (thrombin and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhodophyta; Polysulfated; Gelidiella acerosa; Antithrombotic agent; Bioactivities; In vivo toxicity.
Ano: 2014 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/23704
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Characterization and bioactivities of a novel polysaccharide obtained from Gracilariopsis lemaneiformis Anais da ABC (AABC)
SHI,CHEN-SHAN; SANG,YA-XIN; SUN,GUI-QING; LI,TIAN-YE; GONG,ZHENG-SI; WANG,XIANG-HONG.
ABSTRACT Gracilariopsis lemaneiformis is a type of red alga that contains seaweed polysaccharide agar. In this study, a novel non-agar seaweed polysaccharide fraction named GCP (short of crude polysaccharide obtained from Gracilariopsis lemaneiformis) was isolated from Gracilariopsis lemaneiformis. Structural analysis showed that GCP shows triple helical chain conformation when dissolved in water and has many branches and long side chains. Also, 1→3 linkage is the major linkage and the sugar structures are galactopyranose configurations linked by β-type glycosidic linkages. Two macromolecular substance fractions (GCP-1 and GCP-2) were purified by DEAE Sepharose Fast Flow column chromatography. Moreover, a splenocyte damage assay and splenocyte...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioactivities; Gracilariopsis lemaneiformis; Polysaccharide; Structural characterization..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000100175
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