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| OLIVEIRA,SIMONE S.S.; BELLO,MURILO L.; RODRIGUES,CARLOS R.; AZEVEDO,PAULA L. DE; RAMOS,MARIA C.K.V.; AQUINO-NETO,FRANCISCO R. DE; FIAUX,SORELE B.; DIAS,LUIZA R.S.. |
| ABSTRACT This study presents the bioreduction of six β-ketoesters by whole cells of Kluyveromyces marxianus and molecular investigation of a series of 13 β-ketoesters by hologram quantitative structure-activity relationship (HQSAR) in order to relate with conversion and enantiomeric excess of β-stereogenic-hydroxyesters obtained by the same methodology. Four of these were obtained as (R)-configuration and two (S)-configuration, among them four compounds exhibited >99% enantiomeric excess. The β-ketoesters series LUMO maps showed that the β-carbon of the ketoester scaffold are exposed to undergo nucleophilic attack, suggesting a more favorable β-carbon side to enzymatic reduction based on adopted molecular conformation at the reaction moment. The HQSAR... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biocatalysis; Β-ketoesters; Β-hydroxyesters; HQSAR; Whole cell bioreduction. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000401403 |
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| Dubey,Sachin; Singh,Amit; Banerjee,Uttam C. |
| Growth and nitrilase production by recombinant Escherichia coli cells harbouring pET 21 (b) plasmid, for the expression of Pseudomonas putida nitrilase were improved using response surface methodology. Central composite design was used for obtaining ideal concentration of critical medium components which include fructose, tryptone, yeast extract and lactose. The optimal values for the concentration of fructose, tryptone, yeast extract and lactose were found to be 1.13, 2.26, 3.25 and 0.9 % (w/v), respectively. Here, fructose served as carbon source for the growth while lactose was preferably used as inducer for the expression of foreign protein. Yeast extract in the medium was used as a growth promoter while tryptone was added as a major nitrogen source.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biocatalysis; Recombinant E. coli; Nitrilase; Response surface methodology; Central composite design. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300029 |
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| Ramírez,Mario A.; Pérez,Herminia I.; Manjarrez,Norberto; Solís,Aida; Luna,Héctor; Cassani,Julia. |
| Chiral diarylmethanols are versatile building blocks for the preparation of biologically active substances, but they are difficult to obtain in enantiopure form. We used Nocardia corallina B-276 for the oxidative kinetic resolution of (±)-4-(chlorophenyl)phenylmethanol, 1. Two experimental methods were used: 1) Suspension of cells in a phosphate buffer solution and 2) Cells in the culture media, in a 3-L bioreactor. After 36 hrs using the first method, the ketone/alcohol ratio was 56/44 and the unoxidized alcohol had an enantiomeric ratio of 93/7, predominating the R-alcohol. |
| Tipo: Journal article |
Palavras-chave: 4-chlorobenzophenone; Biocatalysis; Microbial oxidation; Resolution. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400002 |
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| Rodríguez,Paula; Sierra,Wilson; Rodríguez,Sonia; Menéndez,Pilar. |
| The forest industry in Uruguay has grown considerably during the last decade. Eucalyptus plantations account for 74% of the forested land, with Eucalyptus globulus being the most widely distributed species. This industry is dedicated exclusively to the production of wood without exploiting the by-products (leaves and small branches). Eucalyptus leaves are known to contain important amounts of essential oils composed primarily of 1,8-cineole (1,3,3-trymethyl-2-oxabicyclo[2.2.2]octane). In this work, the biotransformation of 1,8-cineole, is achieved using a native bacterium (Rhodococcus sp.) which was isolated from the soil of Eucalyptus forest. A 98% of bioconversion was achieved. Three different optically pure compounds were obtained, and they were... |
| Tipo: Journal article |
Palavras-chave: Biocatalysis; Monoterpenes; Eucalyptus; Green Chemistry. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300011 |
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| VAZ JUNIOR, S.; RODRIGUES, D. de S.; GONCALVES, S. B.; ALMEIDA, J. R. M. de; CARVALHO, W.; PACHECO, T. F.. |
| Os ácidos aldônicos — glicônico e xilônico — estão entre as moléculas de alto valor agregado que podem ser obtidas a partir de biomassa lignocelulósica e apresentam elevado potencial de aplicação comercial em diferentes campos. A síntese de ácidos aldônicos é possível por meio de diferentes processos, como: químico, eletroquímico, microbiano e enzimático. Porém, independentemente da opção escolhida, ainda existem vários obstáculos para que a produção em larga escala seja bem estabelecida. Entre esses obstáculos, está o desenvolvimento de um catalisador eficaz e estável para a síntese dos ácidos aldônicos a partir de hidrolisados de biomassa lignocelulósica, cuja composição apresenta, além dos monômeros de interesse (glicose e xilose), um grande número de... |
| Tipo: Folhetos |
Palavras-chave: Biocatalisador; Ácidos aldônicos; Biocatálise; Reator de leito fixo; Ácido glicônico; Ácido xilônico; Glicose oxidase; Catalisador; Green chemistry; Biocatalysis; Biomass; Glucose oxidase. |
| Ano: 2020 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1124141 |
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