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| Costa, Geraldo Márcio da; UFLA; Paiva, Luciano Vilela; UFLA; Piccoli, Roberta Hilsdorf; Universidade Federal de Lavras; Figueiredo, Demétrio Junqueira; Universidade Federal de Lavras; Pereira, Ulisses de Pádua; UFLA; Silva, Nivaldo da; EV-UFMG. |
| Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis. Three hundred fourty four strains of coagulase-positive Staphylococcus (CPS), isolated from mastitis cases, underwent phenotypic and genotypic tests to evaluate the efficiency of a simplified key, based on phenotypic tests for the discrimination of these microorganisms. The tests consisted of amplification of the femA gene and hemolysis in blood agar, production of acetoin and fermentation of maltose, mannitol and trehalose. Strains that showed negative results in the amplification test of the femA gene or that were not identified as Staphylococcus aureus (S. aureus) by phenotypic tests were tested with the APISTAPH kit... |
| Tipo: Pesquisa laboratorial |
Palavras-chave: 5.05.02.03-4 mastitis pathogens; Characterization; Coagulase test; PCR; Biochemical tests Doenças Infecciosas dos Animais mastitis pathogens; Characterization; Coagulase test; PCR; Biochemical tests. |
| Ano: 2010 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6276 |
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| Ahsani,MR; Mohammadabadi,MR; Shamsaddini,MB. |
| Clostridium perfringens is an important pathogen that provokes numerous different diseases. This bacterium is classified into five different types, each of which capable of causing a different disease. There are various methods for the bacterial identification, many are labor-intensive, time-consuming, expensive and also present low sensitivity and specificity. The aim of this research was to identify the different types of C. perfringens using PCR molecular method. In this study, 130 sheep-dung samples were randomly collected from areas around the city of Kerman, southeastern Iran. After processing and culturing of samples, the produced colonies were morphologically studied, gram stain test was also carried out and the genera of these bacteria were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clostridium perfringens; Multiplex PCR; Biochemical tests; Genetic typing. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400006 |
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