|
|
|
|
|
Heidari,Parviz. |
Abstract Cold stress is one of the limiting factors of plant production that plants use different mechanisms for cold tolerance. CBF genes play critical role to regulate the cold responsive genes. To better understand of CBF gene functions, the tomato-CBFs and Arabidopsis-CBFs were evaluated using bioinformatics tools, and finally the expression patterns of SlCBF1 gene were analyzed under 10 and 4˚C in two contrasting tomato species (Solanum lycopersicum and S. habrochaites). The different cis regulatory elements were observed in promoter region of SlCBF1 and AtCBF1 genes, and ICE1, COR and HOS1 proteins exhibited high interaction with CBFs. The results of Real time PCR of SlCBF1 exhibited that under 10 and 4 ˚C, SlCBF1 was down regulated in cold sensitive... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cold stress; CBF genes; Real time PCR; Tomato; Bioinformatics analysis. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100208 |
| |
|
|
Ser,Hooi-Leng; Tan,Wen-Si; Ab Mutalib,Nurul-Syakima; Yin,Wai-Fong; Chan,Kok-Gan; Goh,Bey-Hing; Lee,Learn-Han. |
ABSTRACT As the largest genus in Actinobacteria family, Streptomyces species have the ability to synthesize numerous compounds of diverse structures with bioactivities. Streptomyces mangrovisoli MUSC 149T was previously isolated as a novel streptomycete from mangrove forest in east coast of Peninsular Malaysia. The high quality draft genome of MUSC 149T comprises 9,165,825 bp with G + C content of 72.5%. Through bioinformatics analysis, 21 gene clusters identified in the genome were associated with the production of bioactive secondary metabolites. The presence of these biosynthetic gene clusters in MUSC 149T suggests the potential exploitation of the strain for production of medically important compounds. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genome sequence; Streptomyces; Mangrove; AntiSMASH; Bioinformatics analysis. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100013 |
| |
|
| |
|
| |
|
|
He,Jin; Yang,Haoyu; Xu,Zhonghua; Li,Jin; Chen,Gang; Jiang,Lifeng; Wu,Lidong; Zhou,Xindie. |
Abstract Paired basic amino acid-cleaving enzyme 4 (PACE4), a proprotein convertase, is involved in the activation of aggrecanases (ADAMTS-4 and ADAMTS-5) in osteoarthritic and cytokine-stimulated cartilage. Activated aggrecanases cause aggrecan degradation and thus, contribute to osteoarthritis (OA). In this study, we investigated the association between PACE4 gene polymorphisms and OA risk. One single-nucleotide polymorphism (rs4965833) in the PACE4 gene was genotyped in 432 OA patients and 523 healthy controls using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Quantitative reverse transcription PCR (qRT-PCR) was used to determine the relative expression of PACE4 in blood samples from 90 OA patients (30 for each... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ACE4; Osteoarthritis; Bioinformatics analysis; Single-nucleotide polymorphism. |
Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100104 |
| |
|
|
|