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Donnees cytologiques, electrophysiologiques et biochimiques relatives au phenomene de fecondation chez le homard (Homarus gammarus ). Implication du processus d'activation sur le mecanisme d'attache des oeufs pondus ArchiMer
Goudeau, M; Lachaise, F; Kubisz, P; Goudeau, H.
Electrophysiological measurements and related cytological controls were done on immature and submature oocytes of Homarus gammarus . By chromatography (HPLC) and radio-immunoassays (RIA) it was established that submature oocytes displayed different ecdysteroids. Furthermore, the structure of the stalk (funiculus) for the laid eggs attachment was analyzed. it consisted of the two superimposed vitelline envelopes which were likely modified by the cortical reaction exudate during the fertilization process. In addition, the successive secretory phases, regarding to the embryonic envelopes were evidenced. Finally, the ecdysteroid titers of embryonic stages were measured.
Tipo: Text Palavras-chave: Homarus gammarus; Malacostraca; Biological attachment; Hormones; Electrophysiology; Biochemistry; Cytology; Eggs; Embryonic development; Cell membranes; Oocytes; Fecundity; Homard; Decapode; Crustacé; Funicule; Mues embryonnaires; Enveloppe de fécondation; Ecdystéroîdes; Potentiel membranaire; Ovocyte.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1190.pdf
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Nutrition experimentale: Problemes methodologiques lies a l'utilisation des microorganismes comme source trophique ArchiMer
Guidi, L.
The present study deals with technical problems encountered under lab conditions in studies of trophic relations between deposit-feeding invertebrates and microorganisms. Using 2 pure cultures of Pseudomonas and Navicula , it was shown that these microorganisms will attach to artificial (glass beads) and natural (sand grains) substrates. Best microbial attachment was observed on sand (up to 4.8 x 10 super(8) cells.g super(-1) dry wt.). The radioactive labelling of the microorganisms depends not only on the form under which the isotope ( super(14)C) is supplied to the cultures, but also on the time and the duration of the labelling. 70% of the isotope was incorporated in a stable manner by the diatoms. The bacteria incorporated, at most, 34% of the...
Tipo: Text Palavras-chave: Navicula; Pseudomonas; Bacillariophyceae; Algae; Bacteria; Biological attachment; Radioactive tracers; Methodology; Microorganisms; Trophic relationships; Microbiological culture; Zoobenthos; Nutrition.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-987.pdf
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