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| Saponji,Svetlana; Kneevi-Jugovi,Zorica D; Bezbradica,Dejan I; Zuza,Milena G; Saied,Omar Ali; Boskovi-Vragolovi,Nevenka; Mijin,Dusan Z. |
| Lipase from Candida rugosa was covalently immobilized on Sepabeads EC-EP for application for amyl caprylate synthesis in an organic solvent system. Several solvents were tested in terms of biocatalyst stability and the best result was obtained with isooctane. The lipase-catalyzed esterification in the selected system was performed in batch and fluidized bed reactor systems. The influence of several important reaction parameters including temperature, initial water content, enzyme loading, acid/alcohol molar ratio, and time of addition of molecular sieves is carefully analyzed by means of an experimental design. Almost complete conversion (> 99%) of the substrate to ester could be performed in a batch reactor system, using lipase loading as low as 37 mg... |
| Tipo: Journal article |
Palavras-chave: Bioreactors; Covalent immobilization; Ester; Non-aqueous system; Optimization technique. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600012 |
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| Scheidt,Gessiel Newton; Arakaki,Andréa Haruko; Chimilovski,Jacqueline Sugitani; Portella,Augustus Caeser Franke; Spier,Michele Rigon; Woiciechowski,Adenise Lorenci; Biasi,Luiz Antonio; Soccol,Carlos Ricardo. |
| The research for new techniques of in vitro cultivation is being object of many studies around the world, in order to optimize and decrease production costs of seedlings with agronomical interest. The main goal of this work was to compare different systems of in vitro cultivations using Ananas comosus L. Merril. So, the in vitro growth of the plantlets was promoted in two different bioreactors: Bioreactor of Immersion by Bubbles (B.I.B.®) and the Reactor of Temporary Immersion (R.I.T.A.®) with immersion cycle every 2 hours for 15 minutes and the traditional system in flasks with 200 mL. All cultivation systems used the MS liquid nutritive solution, supplemented with BAP (1 mgL-1), ANA (0.25 mgL-1), sucrose (30 gL-1) and Tween 20® (0.5 µL). The pH was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioreactors; Ananas comosus; Micro propagation. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700005 |
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