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	Provedor de dados BJM (1) Autor Leite,José Paulo Gagliardi (1) Nascimento,Jussara Pereira do (1) Spano,Liliana Cruz (1) Vargas,Paulo Roberto Merçon de (1) Palavra-chave Biotinylated probes (1) Citomegalovirus (1) Endogenous biotin (1) Glandular epithelium of decidua (1) In situ hybridisation (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2005 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última False positive reaction due to endogenous biotin activity in glandular epithelium of decidua BJM Spano,Liliana Cruz; Vargas,Paulo Roberto Merçon de; Leite,José Paulo Gagliardi; Nascimento,Jussara Pereira do. Biotin-labeled probe was used in an in situ hybridisation assay to localize virus infection in formalin-fixed, paraffin embedded tissues taken from eleven abortion cases. Probes for human cytomegalovirus (HCMV), human Parvovirus B19 (B19) and human adenovirus type 2 (HAd2), were labeled with biotin-11-dUTP by nick-translation reaction. Streptavidin-alkaline-phosphatase (SAP) was used to detect biotin, followed by 4-nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) solution. Positive reaction was observed in nucleus of glandular ephitelium cells of decidua either in positive or in negative control at first and second gestational trimester. The reaction was not inhibited with blocking solution for alkaline phosphatase endogenous activity... Tipo: Info:eu-repo/semantics/article Palavras-chave: Endogenous biotin; Glandular epithelium of decidua; Biotinylated probes; In situ hybridisation; Citomegalovirus. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200002 Registros recuperados: 1 Primeira ... 1 ... Última

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