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Production of a bioherbicide agent in liquid and solid medium and in a biphasic cultivation system Planta Daninha
Moraes,C.; Monteiro,A.C.; Machado,A.C.R.; Barbosa,J.C.; Mochi,D.A..
The use of fungi in weeds control programs depends upon the conidia production in large scale. Therefore, this study aimed to evaluate liquid and solid culture media and the cultivation by biphasic system for the conidia production of Bipolaris euphorbiae Muchovej & Carvalho a specific pathogen of Euphorbia heterophylla. The liquid media were obtained from agro-industrial waste or by-products, and the solid media were prepared with mixtures of grains and grain derivatives. The liquid medium made with sugar cane molasses stood out from the others because it provided great sporulation (23 x 10(4) conidia mL-1 of medium), conidial viability (99.7%), and formation of mycelial fungal biomass (1.26 g 100 mL-1 of medium). On solid media conidial...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bipolaris euphorbiae; Euphorbia heterophylla; Biological control; Weed control; Fungus production; Natural substrates.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582014000200002
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Phytotoxin produced by Bipolaris euphorbiae in-vitro is effective against the weed Euphorbia heterophylla BABT
Barbosa,Aneli M.; Souza,Cristina G. M.; Dekker,Robert F. H.; Fonseca,Rafael C.; Ferreira,Dalva T..
Four virulent strain isolates of the fungus, Bipolaris euphorbiae (previously identified as a Helminthosporium sp.), isolated from host plants in four states within Brazil were screened for the production of phytotoxins that promoted wilting and defoliation of the Brazilian weed, Euphorbia heterophylla, commonly found growing among soyabean crops. Only one isolate, B. euphorbiae Strain I (EUPH petropar from Mato Grosso state), produced phytotoxin in-vitro when grown in stationary culture for 7 d at 28 ° C on minimum salts medium supplemented with 1.5 % glucose as the sole carbon source. Phytotoxin was also produced when the fungal strain was grown on fructose, galactose, mannose, xylose and sucrose. The addition of nitrogen source (yeast extract, peptone...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bipolaris euphorbiae; Euphorbia heterophylla; Phytotoxin production; Phytotoxin stability.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200016
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Germination and mycelial growth of Bipolaris euphorbiae Muchovej & Carvalho as influenced by herbicides and surfactants BJM
Nemoto,Marta Cecília Mallasen; Nahas,Ely; Pitelli,Robinson Antonio; Coelho,Lisias.
Bipolaris euphorbiae Muchovej & Carvalho can be combined with herbicide in order to control a large spectrum of weed species, being a strong candidate for the biocontrol of Euphorbia heterophylla L. (milk weed). The fungus release can be combined with herbicide in order to control a broader spectrum of weed species. Thus, laboratory experiments were set up to study the feasibility of using tank mixes of B. euphorbiae spores with herbicides or surfactants recommended for soybean. Mycelial growth and conidia germination were evaluated in PDA medium enriched with the herbicides oxasulfuron (80 g/ha), glyphosate (4 L/ha), bentazon (1.5 L/ha), fomesafen (1 L/ha), chlorimuron-ethyl (80 g/ha), lactofen (1 L/ha) and imazetaphyr (1 L/ha), and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bipolaris euphorbiae; Mycelial growth; Conidia germination; Herbicides; Surfactants.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400015
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