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	Provedor de dados Electron. J. Biotechnol. (1) Autor Arruga,M. Victoria (1) García,Cristina B (1) Postiglioni,Alicia (1) Rincón,Gonzalo (1) Palavra-chave Bisulfite (1) Collagen (1) Creole cattle (1) Cytosines (1) Methylation (1) Tipo do documento Journal article (1) Ano 2011 (1) País Chile (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Methylation-specific PCR analysis in Col8A1 promoter in Creole cattle carrier of rob(1;29) Electron. J. Biotechnol. Postiglioni,Alicia; García,Cristina B; Rincón,Gonzalo; Arruga,M. Victoria. Robertsonian translocation (rob(1;29)) is the most frequent structural chromosomal abnormality in cattle. Heterozygous carriers have a normal phenotype but show a 3-5% decrease in fertility. Chromatin decondensation was evaluated similar to the inactive X chromosome when submitted to demethylating agent. Based on this result, and the concept that imprinted genes are essential in embryonic development, we decided to query genes located on BTA1 and BTA29 that could undergo genome imprinting. The collagen typeVIII-α1 (Col8A1) acted on extracellular matrix structural proteins. DNA bisulfite conversion and sequentiation methods were used to compare its differential methylation patterns. It was performed on eight Creole cattle DNA blood samples from normal and... Tipo: Journal article Palavras-chave: Bisulfite; Collagen; Creole cattle; Cytosines; Methylation. Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300013 Registros recuperados: 1 Primeira ... 1 ... Última

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