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Registros recuperados: 22
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Use of dried waste of cassava starch extraction for feeding lactating cows Anais da ABC (AABC)
FERNANDES,TATIANE; ZAMBOM,MAXIMILIANE A.; CASTAGNARA,DEISE D.; SOUZA,LEILIANE C.; DAMASCENO,DAIANE O.; SCHMIDT,EMERSON L..
The aim of this study was to determine the best level of utilization of dried waste of cassava starch extraction (WCSEd) as a substitute for corn for lactating cows. Four lactating cows were fed diets with increasing levels (0%, 33%, 66% and 100%) of WCSEd as a substitute for corn. The intake and digestibility of dry matter and nutrients, milk production and composition, blood parameters of glucose and urea and microbial synthesis of the diets were evaluated. There was a reduction in dry matter intake, organic matter, ether extract and total carbohydrate, and increased intake of acid detergent fiber. Nutrient digestibility was not affected while the synthesis of microbial protein increased. These changes resulted in reduced milk production, without...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood parameters; Digestibility; Intake; Microbial synthesis; Production.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000201101
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Monitoring of physiological and blood parameters during perinatal and neonatal period in calves Arq. Bras. Med. Vet. Zootec.
Piccione,G.; Casella,S.; Pennisi,P.; Giannetto,C.; Costa,A.; Caola,G..
Rectal temperature, heart and respiratory rates, and the course of some blood parameters were monitored in calves during perinatal and neonatal periods. The study was carried out on eight Limousine calves. From all subjects, rectal temperature, heart rate, and respiratory rate were measured. Blood samples were taken at the same hour (9am) from the external jugular vein, and then centrifuged and stored until analyses. By means of an UV spectrophotometer, the following blood parameters were assessed for each subject: total protein, total cholesterol, triglycerides, glucose, non-esterified fatty acids (NEFA), urea, creatinine, cholinesterase, lactate dehydrogenase (LDH), hydroxybutirate, glutamate pyruvate transaminases (GPT), glutamate oxalacetate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Calf; Neonatal period; Perinatal period; Blood parameters; Physiological parameters.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000100001
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Effects of chito-oligosaccharides and L-carnitine supplementation in diets for Japanese quails on performance, carcass traits and some blood parameters Arq. Bras. Med. Vet. Zootec.
Tufan,T.; Arslan,C.; Durna,Ö.; Önk,K.; Sari,M.; Erman,H..
The aim of this study was to determine effects of dietary supplementation with chitosanoligosaccharides (COS) and L-carnitine, individually or dually, on growth performance, carcass traits and some blood serum parameters in quails. A total of 192, four days old, Japanese quail chicks were allotted four groups, each of which included four replicates (12 birds per replicate). The groups received the same basal diet supplemented with 0 (Control), 150mg/kg chitosanoligosaccharides (COS), 150mg/kg L-carnitine (Carnitine), and 150 mg/kg chitosanoligosaccharides+150 mg/kg L-carnitine (COS+Car.) during the starter (1 to 21 days) and a grower (22 to 42 days) period. The feeding trial shoved that COS, L-carnitine and COS+L-carnitine had no significant effect on live...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Quail; Chitosanoligosaccharides; L-carnitine; Growth performance; Carcass traits; Blood parameters.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000100283
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Physiological responses of rabbits fed with diets containing rapeseed meal, white lupine and pea seeds as soybean meal substitutes Ciência e Agrotecnologia
Gugołek,Andrzej; Juśkiewicz,Jerzy; Kowalska,Dorota; Zwoliński,Cezary; Sobiech,Przemysław; Strychalski,Janusz.
ABSTRACT In recent years, a trend has emerged to eliminate soybean meal (SBM) from rabbit diets. It was hypothesized that a dietary mixture composed of rapeseed meal (RSM), white lupine seeds (WLS) and pea seeds (PS) could be a substitute for SBM in diets for growing rabbits without compromising their growth and physiological parameters. To verify this hypothesis, selected parameters describing the growth rate of rabbits, their blood and slaughter parameters and caecum function were analyzed. The experiment was performed on ninety HYPLUS rabbits. Control group (C) animals were fed a diet containing 15% SBM. The diet administered to the first experimental group (E1) contained 7.5% SBM, 5.0% RSM, 4.0% WLS and 3.0% PS. In the diet fed to the second...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rabbit feeding; Growth performance; Blood parameters; Gut function.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542018000300297
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Effect of different periods of pre-slaughter stress on the quality of the Nile tilapia meat Ciênc. Tecnol. Aliment.
CASTRO,Pedro Luiz de; LEWANDOWSKI,Vanessa; SOUZA,Maria Luiza Rodrigues de; CORADINI,Melina Franco; ALEXANDRE,Alciony Andréia da Cunha; SARY,César; RIBEIRO,Ricardo Pereira.
Abstract The study was carried out with the objective of evaluating the influence of pre-slaughter stress on meat quality of Nile tilapia (O. niloticus) for 10 days of storage in ice. 75 whole fish were used eviscerated, with average weight and length of 825.75 ± 101.59 g and 28.25 ± 0.81 cm, respectively. The treatments consisted of time in which the animals underwent pre-slaughter hypoxia, being 0, 3 and 6 minutes. Rigor mortis and muscle pH were assessed besides the application of the Quality Index Method and analysis of total protein and blood glucose. Rigor mortis did not differ among treatments during the storage period, and after three hours all animals were in rigor stage. The muscle pH values varied widely during the study period, observing higher...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hypoxia; PH; Rigor mortis; Quality index method; Blood parameters.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000100052
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Anesthetic induction and recovery time of Centropomus parallelus exposed to the essential oil of Aloysia triphylla Ciência Rural
Parodi,Thaylise Vey; Santos,Celina Alcoforado dos; Veronez,Alexandra; Gomes,Levy de Carvalho; Heinzmann,Berta Maria; Baldisserotto,Bernardo.
ABSTRACT: This study analyzed the effectiveness of the essential oil of Aloysia triphylla (EOA) as an anesthetic and stress reducing agent in the transport of fat snook ( Centropomus parallelus ). Juveniles were exposed to different concentrations of EOA (seawater-adapted: 25-300μL L-1; freshwater-adapted: 200μL L-1) to identify the anesthetic induction and recovery times. In an additional experiment, seawater-adapted fat snook were transported in plastic bags with 10 or 20μL L-1 EOA for 6 or 24h. The increased concentration of EOA proportionally decreased the time required for anesthesia induction. Mortality was 30 and 70% in fat snook transported with 10 and 20µL L-1, respectively, 24h after transport. The addition of 20µL L-1 EOA significantly reduced...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anesthesia; Blood parameters; Fat snook; Natural product; Transport.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001202142
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Hematological profile of captive bearded capuchin monkeys (Sapajus libidinosus) from Northeastern Brazil Ciência Rural
Ferreira,Adriano Fernandes; Queiroga,Felisbina Luísa; Mota,Rinaldo Aparecido; Rêgo,Eneida Willcox; Mota,Stéphanie Machado; Teixeira,Magda Guedes; Colaço,Aura.
ABSTRACT: Bearded Capuchin or Black-striped Capuchin monkeys (Sapajus lidibinosus) are New World robust capuchin monkeys widely used in medical research. Few data are available concerning hematological reference values for these species, with no studies available from the Northeast region in Brazil. The aim of this study was to determine the hematological reference values for healthy bearded capuchin monkeys and to analyze the influence of sex and age factors. Blood samples were collected from 50 healthy bearded capuchin monkeys housed in captivity. These were analysed for total erythrocyte, hemoglobin, leukocyte and platelet count, packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sapajus libidinosus; Hematology; Blood parameters; Reference interval..
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001000400
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Feeding strategies and energy to protein ratio on tambaqui performance and physiology PAB
Santos,Marcio Quara de Carvalho; Lima,Mariana do Amaral Camara; Santos,Luciana dos; Pereira-Filho,Manoel; Ono,Eduardo Akifumi; Affonso,Elizabeth Gusmão.
The objective of this work was to evaluate the effect of feed deprivation and refeeding with diets containing different energy to protein ratios (E/P) on the performance and physiology of juvenile tambaqui (Colossoma macropomum). A 4x2 factorial arrangement with three replicates was used, with four E/P ratios (11.5, 10.5, 9.5, and 8.5 kcal g-1 digestible energy per protein) and two feeding regimens (with and without deprivation), during 60 days. Fish from the food-deprived group were fasted for 14 days and refed from the fifteenth to the sixtieth day, whereas the remaining fish were fed for 60 days. At the end of the experimental period, weight of fish subjected to food deprivation was lower than that of those continuously fed; however, this condition did...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Colossoma macropomum; Blood parameters; Energy/protein requirement; Food restriction.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000800024
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Effects of Different Types of Red Ginseng Marc and Fermented Red Koji Blend as Feed Additives on Blood Parameters and Egg Yolk Fatty Acid Profiles of Laying Hens Rev. Bras. Ciênc. Avic.
Lee,GD; Choi,IH.
ABSTRACT The purpose of this study was to evaluate the effects of three different forms (powder, pellet, and coated pellet) of red ginseng marc (RGM) and fermented red koji (FRK) feed additive on the blood parameters and fatty acid (FA) profiles of laying hens, after two months of dietary supplementation. A number of 240 40-week-old Hy-line Brown laying hens were randomly designated to four dietary treatments, each with six replicates of 10 hens each, in a completely randomized design. The control group was fed a basal diet, and the other three treatments groups were fed 1% RGM powder mixed with FRK, pellets of 1% RGM with FRK, and coated pellets of 1% RGM with FRK. The powder and coated pellet diets contained the lowest total cholesterol and glucose...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Red ginseng marc; Fermented redkoji; Blood parameters; Fatty acid profiles.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000300441
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Effect of Sources and Levels of Dietary Zinc on the Performance, Carcass Traits and Blood Parameters of Broilers Rev. Bras. Ciênc. Avic.
Zakaria,HA; Jalal,M; AL-Titi,HH; Souad,A.
ABSTRACT A total of 400 one-day-old, straight-run, commercial (Ross 308) broiler chicks were used to evaluate the effects of two dietary levels of zinc (Zn) sources on broiler chick performance, carcass traits and blood parameters. Corn-soybean diets were formulated for three rearing phases (starter, grower and finisher). The two dietary treatments applied consisted of the addition per kg of diet of 80mg of inorganic Zn (ZnO) (T1), or 80mg of ZnO plus 42mg of an organic Zn-amino acid complex (Availa-Zn120; Zinpro Corporation, Eden Prairie, MN, USA), totaling 122mg of the combined organic and inorganic Zn sources (T2). Birds were distributed according to a completely randomized design in the two treatments with eight replicates (pens) of 25 birds each. Feed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Broilers; Performance; Carcass; Blood parameters; Zinc sources and levels.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000300519
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Influence of sex, age, and fasting on blood parameters and body, bursa, spleen and yolk sac weights of broiler chicks Rev. Bras. Ciênc. Avic.
Pires,DL; Malheiros,EB; Boleli,IC.
The effects of water and feed fasting for 24, 48 and 72 hours post-hatching on blood parameters (mean corpuscular volume, MCV; red blood-cell, RBC; hematocrit, HCT; hemoglobin, HGB; plasma glucose, CGP; plasma total protein, PP, and differential leukocytes count), and on body, liver, spleen, bursa, and yolk sac weights were analyzed. Erythrogram data were obtained with a blood cell counter. Total plasma protein and plasma glucose were determined by using the Bradford method (1976) and a glucose PAP liquiform kit (Labtest, cat. n. 84), respectively. Specific leukocyte counts were carried out on blood smears stained with Rosenfeld solution. According to the obtained data, water and feed post-hatching fasting reduced MCV values, which also were lower in males...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood parameters; Broiler chicks; Bursa of Fabricius; Post-hatching fasting.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2007000400005
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Effect of Various Concentrations of an Anise Seed Powder (Pimpinella Anisum L.) Supplement on Selected Hematological and Biochemical Parameters of Broiler Chickens Rev. Bras. Ciênc. Avic.
Al-Shammari,KIA; Batkowska,J; Gryzińska,MM.
ABSTRACT The objective of the study was to investigate the influence of anise seed (Pimpinella anisum L.) powder supplemented to the drinking water on selected blood parameters of broilers. In total, 360 one-day-old Hubbard Classic chicks were randomly divided into four groups with three replicates of 30 birds each. Anise seed powder was added at 0, 500, 750, and 1000mg/L to the drinking water offered to the C (control), T1, T2, and T3 groups respectively. The supplement was supplied for 56 days. When broilers were 28 and 56 days old, blood samples were collected (30 birds per group) by brachial vein puncture to evaluate the cellular components of blood (RBC, WBC, Hgb, HCT, H/L). The following serum biochemical parameters were evaluated: CHOL, GLU, TP,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood parameters; Poultry; Medicinal plants; Pimpinella anisum L.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000100041
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Hematological values and body, heart and liver weights of male and female broiler embryos of young and old breeder eggs Rev. Bras. Ciênc. Avic.
Morita,VS; Boleli,IC; Cargnelutti Filho,A.
This study analyzed broiler breeder age (29 or 60 wk-old) effects on physical characteristics of eggs (initial mass, mass loss, volume, diameter, surface area and density) and of eggshells(weight, volume, thickness, conductance, and porosity ), as well as the influence of embryo sex on hematological parameters and body, liver and heart weights during incubation (at days 13, 15, 18 and 21). Physical parameter values were lower in 29-wk-old broiler breeder eggs than those of 60-wk-old breeders, except for relative eggshell weight, which was higher. In both male and female embryos, erythrocytic parameters and the body, liver, and heart weights increased during the incubation. The embryos and their organs were heavier when derived from 60-wk-old breeder eggs...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood parameters; Breeder age; Embryos; Eggshell porosity and conductance; Sex.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000100002
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Effects of Kluyveromyces marxianus Isolated from Tibetan Mushrooms on the Plasma Lipids, Egg Cholesterol Level, Egg Quality and Intestinal Health of Laying Hens Rev. Bras. Ciênc. Avic.
Zhong,S; Liu,H; Zhang,H; Han,T; Jia,H; Xie,Y.
ABSTRACT The effects of the Kluyveromyces marxianus M3 strain, isolated from Tibetan mushrooms, on plasma lipids, egg cholesterol level, egg quality, and intestinal health of laying hens were evaluated. In total, 160 Beijing fatty laying hens (43 weeks old) were divided into four groups and fed a basal diet supplemented with 0%, 0.1%, 0.3%, or 0.5% freeze-dried K. marxianus M3 powder for four weeks. The results showed that yeast supplementation reduced serum total cholesterol (TC), triglyceride (TG), low density lipoprotein-cholesterol (LDL-C), and very low density lipoprotein-cholesterol (VLDL-C) levels (p<0.01), and increased serum high density lipoprotein-cholesterol (HDL-C) level (p<0.05). Moreover, regardless of K. marxianus M3 dietary addition...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood parameters; Cholesterol; Egg production; Poultry nutrition; Serum.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000200261
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LIPID METABOLISM INDICES AND FATTY ACIDS PROFILE IN THE BLOOD SERUM OF BROILER CHICKENS FED A DIET WITH LIGNOCELLULOSE Rev. Bras. Ciênc. Avic.
Bogusławska-Tryk,M; Piotrowska,A; Szymeczko,R; Burlikowska,K; Głowińska,B.
ABSTRACT The aim of the research was to determine lipid metabolism indices and fatty acid profile in the blood serum of Ross 308 chickens (n = 48), fed a finisher mixture supplemented with 0, 0.25, 0.5 and 1.0% of lignocellulose. The feeding trial lasted from 21 to 42 d of the birds' age. Blood samples were collected from each chicken at 42d of age from the pterygoid canal vein. In the blood serum the content of triglycerides (TG), total cholesterol (TCHOL) and high density lipoprotein (HDL) fraction was determined by the spectrophotometric method. The fatty acids concentration was estimated with the use of the gas chromatography method. Lignocellulose in doses of 0.5 and 1.0% significantly reduced the concentration of triglycerides and low density...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood parameters; Broiler; Fatty acids; Fiber.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000300451
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Effect of protein, carbohydrate, lipid, and selenium levels on the performance, carcass yield, and blood changes in broilers Rev. Bras. Ciênc. Avic.
Hada,FH; Malheiros,RD; Silva,JDT; Marques,RH; Gravena,RA; Silva,VK; Moraes,VMB.
The objective of this study was to evaluate the performance, carcass and parts yield, and blood changes in broilers fed different protein, carbohydrate, and lipid levels. Birds were fed a commercial diet until seven days of age. On day 8, birds were distributed according to a completely randomized experimental design in a 4 x 2 factorial arrangement (control diet, low protein diet, low carbohydrate diet or low lipid diet vs. supplementation of 0 or 0.3ppm organic selenium) with four replicates of 15 birds each. Broilers fed low protein presented lower body weight, feed intake, and worse feed conversion ratio on day 42, as well as lower carcass and breast yields, higher leg and abdominal fat yields, higher triglyceride and lower uric acid blood levels....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbohydrates; Lipids; Blood parameters; Protein; Selenium.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2013000400014
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Effect of humic acid on survival, ionoregulation and hematology of the silver catfish, Rhamdia quelen (Siluriformes: Heptapteridae), exposed to different pHs Rev. Bras. Zool.
Costa,Silvio T. da; Gressler,Luciane T.; Sutili,Fernando J.; Loebens,Luíza; Lazzari,Rafael; Baldisserotto,Bernardo.
This study evaluates whether humic acid (HA; Aldrich) protects the silver catfish, Rhamdia quelen (Quoy & Gaimard, 1824), against exposure to acidic pH. Survival, levels of Na+, Cl- and K+ plasma, hematocrit, hemoglobin and erythrocyte morphometry were measured. Fish were exposed to 0, 10, 25 and 50 mg L 1 HA at four pH levels: 3.8, 4.0, 4.2 and 7.0 up to 96 hours. None of the fish exposed to pH 3.8 survived for 96 hours into the experiment, and survival of fish subjected to pH 4.0 decreased when HA concentration increased. Plasma Na+ levels decreased when pH was acidic, with no influence of HA, while Cl- levels declined at low pH with increased HA concentration. The levels of K+ at pH 4.0 and 4.2 increased without HA. Hematocrit and hemoglobin...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood parameters; Humic acid; Plasma ion levels; Survival.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702015000300215
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Passive immune transfer, health, pre-weaning performance, and metabolism of dairy calves fed a colostrum supplement associated with medium-quality maternal colostrum R. Bras. Zootec.
Paula,Marília Ribeiro De; Rocha,Nathália Brito; Miqueo,Evangelina; Silva,Fernanda Lavínia Moura; Coelho,Marina Gavanski; Bittar,Carla Maris Machado.
ABSTRACT The objective of this study was to evaluate passive immunity transfer, health, performance, and metabolism of Holstein calves fed colostrum supplement associated with medium-quality colostrum. After birth, calves were blocked according to birth weight and date and distributed into the following treatments: high-quality colostrum (> 50 mg of Ig/mL; n = 15; 150 g kg−1 BW; positive control group); medium-quality colostrum (30-40 mg of Ig/mL; n = 14; 150 g kg−1 BW), and medium-quality colostrum (30-40 mg of Ig/mL; n = 15; 150 g kg−1 BW) + colostrum supplement (15 mL) given with the colostrum. Colostrum was given within the first 12 h of life in two meals by nipple bottle, and colostrum replacer was administered by a blister syringe. Blood samples...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood parameters; Colostrum supply; Immunity; Immunoglobulin Y; Intake.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100717
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Effects of dietary addition of synbiotic on the performance, carcass traits, and serum parameters of Japanese quails R. Bras. Zootec.
Tufan,Tuncay; Bolacali,Memis.
ABSTRACT This study aimed to evaluate the effects of different levels of synbiotic (Syn) on the growth performance, carcass traits, and serum parameters of Japanese quails. A total of 1,500 (750 male and 750 female) Japanese quails were assigned to five treatments, including the control (basal diet) and basal diets with the addition of 0.1, 0.2, 0.3, and 0.4% Syn according to a completely randomized design. Each treatment in the male and female experiment groups consisted of five replicates, with 30 chicks for each replicate. The addition of Syn had no effect on body weight (BW), while the BW of female quails was higher than that of male quails at day 42. The addition of Syn reduced feed intake (FI) and improved the feed conversion ratio (FCR)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood parameters; Carcass attributes; Growth performance; Synbiotics; Quail.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982017001000805
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Evaluation of mannan-oligosaccharides offered in milk replacers or calf starters and their effect on performance and rumen development of dairy calves R. Bras. Zootec.
Silva,Jackeline Thaís da; Bittar,Carla Maris Machado; Ferreira,Lucas Silveira.
The objective of this study was to evaluate the route of administration of mannan-oligosaccharides in the diet of dairy calves and their effects on performance and plasma parameters indicative of rumen development. Following birth, twenty-four male Holstein calves were used in a completely randomized design and assigned to the following treatments: Control; 4 g/d Bio-Mos® (Alltech Biotech.) added to starter concentrate; and 4 g/d Bio-Mos® mixed into milk replacer. Animals were housed in individual hutches with free access to water, and fed 4L/d of milk replacer until weaning at six weeks. Calves also received 23g/kg crude protein of starter concentrate ad libitum. Fecal scores were evaluated daily. Body weights, growth measurements and blood samples for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Additives; Blood parameters; Early weaning; Fecal score.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000300038
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