Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados Acta biol.Colomb. (1) BJID (1) Autor BOHORQUEZ ÁVILA,Sonia del Pilar (1) Botão,Gislene Diógenes (1) CAMACHO ORTEGA,Sigrid Johanna (1) CASTELLANOS PARRA,Jaime Eduardo (1) Cogo,Laura Lúcia (1) Dalla Costa,Libera Maria (1) Daur,Alessandra Valle (1) Klimak Jr.,Francisco (1) Monteiro,Cristina Leise Bastos (1) VELANDIA ROMERO,Myriam Lucia (1) Mais... Palavra-chave Body fluids (2) Cell culture (1) Cytomegalovirus (1) Enrichment (1) Etiological agents (1) Isolation (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2019 (1) 2006 (1) País Brazil (1) Colombia (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última ISOLATION OF THE HUMAN CYTOMEGALOVIRUS FROM BODILY FLUIDS Acta biol.Colomb. CAMACHO ORTEGA,Sigrid Johanna; BOHORQUEZ ÁVILA,Sonia del Pilar; VELANDIA ROMERO,Myriam Lucia; CASTELLANOS PARRA,Jaime Eduardo. ABSTRACT In vitro studies on the pathogenesis of the human cytomegalovirus (HCMV) are conducted regularly using laboratory adapted strains that lose some characteristics during the adaptation process. Since HCMV is excreted from bodily fluids during infection or reactivation, this work aimed to isolate and culture HCMV from the MRC-5 human cells found in the urine, bronchoalveolar lavage, saliva, and plasma samples of pediatric patients with probable or confirmed infection. The samples were inoculated on cell cultures either for 14 days or until a cytopathic effect (CPE) of 80 % was observed. The cell lysates and supernatants were used to perform successive viral passages. Besides HCMV, the herpes simplex virus was detected from all the saliva samples.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Body fluids; Cell culture; Cytomegalovirus; Isolation. Ano: 2019 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2019000300520 Enrichment methodology to increase the positivity of cultures from body fluids BJID Daur,Alessandra Valle; Klimak Jr.,Francisco; Cogo,Laura Lúcia; Botão,Gislene Diógenes; Monteiro,Cristina Leise Bastos; Dalla Costa,Libera Maria. Isolation and identification of etiological agents found in body fluids can be of critical importance for the recovery of patients suffering from potentially-severe infections, which are often followed by serious sequels. Eighty-two samples of different body fluids were analyzed using two different methods: (1) the conventional culture method (agar plating) and (2) the enrichment culture technique, using the Bact/Alert® blood culture bottle. The number of positive cultures increased on average from 9.7% to 23.1% with the enrichment culture technique. Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus were the most frequently isolated bacteria. The enrichment method could provide a more accurate means the identifying etiological agents. Tipo: Info:eu-repo/semantics/article Palavras-chave: Body fluids; Etiological agents; Enrichment. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000600002 Registros recuperados: 2 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco