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Validity of the polymerase chain reaction in the diagnosis of clinically suspected cases of American visceral leishmaniasis BJID
Pedrosa,Celia Maria Silva; Ximenes,Ricardo Arraes de Alencar; Almeida,Wendell Alexandre Pinheiro de; Rocha,Eliana Maria Mauricio da.
To test the validity of the polymerase chain reaction for diagnosing American visceral leishmaniasis, 88 suspected cases were studied. Diagnosis was confirmed in 47 (53.5%) and ruled out in 41 (46.5%) patients. Samples of bone marrow and peripheral blood were processed by polymerase chain reaction to evaluate the sensitivity and specificity of the test and its agreement beyond chance with microscopy examination. The polymerase chain reaction was positive in bone marrow of 100% of the patients with amastigotes seen with microscopy examination, and in 59.5% in those where no parasite were seen. Agreement beyond chance between visualization of the parasite in bone marrow aspirates and polymerase chain reaction was considered weak (Kappa = 0.41). Concordance...
Tipo: Info:eu-repo/semantics/article Palavras-chave: American visceral leishmaniasis; Diagnosis; Polymerase chain reaction; Peripheral blood; Bone marrow blood.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000300003
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