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	Provedor de dados Ciênc. Tecnol. Aliment. (1) Anais da ABC (AABC) (1) Autor Andrella,Raquel R.S. (1) Araújo,Edílson Divino de (1) Barth,Ortrud M. (1) Cardozo,Gina M.B.Q. (1) Freitas,Alex S. (1) Jain,Sona Arun (1) Jesus,Flávia Thalita de (1) Maester,Fernanda M. (1) Marchioro,Giulia Manso (1) Oliveira,Érika S. (1) Silva,Rosana A. (1) Mais... Palavra-chave Botanical origin (2) Apis (1) Commercial pollen batches (1) Honey pollen (1) Molecular approach (1) Pollen analysis (1) Pollen loads (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2013 (1) 2010 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Evaluation of the botanical origin of commercial dry bee pollen load batches using pollen analysis: a proposal for technical standardization Anais da ABC (AABC) Barth,Ortrud M.; Freitas,Alex S.; Oliveira,Érika S.; Silva,Rosana A.; Maester,Fernanda M.; Andrella,Raquel R.S.; Cardozo,Gina M.B.Q.. High quality of bee pollen for commercial purpose is required. In order to attend the consumer with the best identification of the botanical and floral origin of the product, 25 bee pollen batches were investigated using two techniques of pollen grain preparation. The first started to identify pollen loads of different colors in two grams of each well mixed batch, and the second to identify pollen grains in a pool made of all the pollen loads comprised in two grams. The best result was obtained by this last technique, when a pollen grain suspension was dropped on a microscope slide and circa 500 pollen grains were counted per sample. This analysis resulted in the recognition of monofloral and bifloral pollen batches, while the use of the first technique... Tipo: Info:eu-repo/semantics/article Palavras-chave: Apis; Pollen loads; Commercial pollen batches; Pollen analysis; Botanical origin. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000400011 Extraction of DNA from honey and its amplification by PCR for botanical identification Ciênc. Tecnol. Aliment. Jain,Sona Arun; Jesus,Flávia Thalita de; Marchioro,Giulia Manso; Araújo,Edílson Divino de. The physiochemical and biological properties of honey are directly associated to its floral origin. Some current commonly used methods for identification of botanical origin of honey involve palynological analysis, chromatographic methods, or direct observation of the bee behavior. However, these methods can be less sensitive and time consuming. DNA-based methods have become popular due to their simplicity, quickness, and reliability. The main objective of this research is to introduce a protocol for the extraction of DNA from honey and demonstrate that the molecular analysis of the extracted DNA can be used for its botanical identification. The original CTAB-based protocol for the extraction of DNA from plants was modified and used in the DNA extraction... Tipo: Info:eu-repo/semantics/article Palavras-chave: Botanical origin; Honey pollen; Molecular approach. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000400022 Registros recuperados: 2 Primeira ... 1 ... Última

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