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| Jerez,José Antonio; Gregori,Fábio; Brandão,Paulo Eduardo; Rodriguez,Cesar Alejandro Rosales; Ito,Fumio Honma; Buzinaro,Maria da Glória; Sakai,Takeo. |
| Isolation of BCoV was performed in monolayers of HmLu-1 cells, using 20 fecal samples from clinical cases of diarrhea in calves. Samples were positive for BCoV by means of hemagglutination / hemagglutination inhibition (HA/HI). Up to the fifth serial passage, 13 of these isolates presented syncytial cytopathic effect, similar to Kakegawa standard strain. When isolates were submitted to seroneutralization with gammaglobulin anti-bovine coronavirus, 8 of them were considered to be positive, once cytopathic effect was neutralized. After titration and seroneutralization in microplates, only three of them were confirmed as positive. The lineage HmLu-1 showed higher permissivity to BCoV isolation, but the low intensity of viral replication demonstrated that new... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine coronavirus (BCoV); Isolation; HmLu-1 cells. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300001 |
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