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| 松山, 雄喜; 神尾, 恭平; 村上, 智亮; 下田, 崇; 古林, 与志安; 古岡, 秀文; 松本, 高太郎; 猪熊, 壽; MATSUYAMA, Yuuki; KAMIO, Kyouhei; MURAKAMI, Tomoaki; SHIMODA, Takashi; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; MATSUMOTO, Kohtaro; INOKUMA, Hisashi. |
| 5 歳6 カ月齢ホルスタイン種乳牛が，後躯麻痺による起立不能を呈した．浅頸リンパ節の腫大と直腸検査による腫瘤 の触知から牛白血病を疑ったが，血液検査とリンパ節の針吸引生検では確定診断できなかった．剖検では，60 × 40 × 20cm 大の多結節性腫瘤が背側の腹腔壁に接して存在していた．腫瘍組織は腸腰筋に浸潤し，一部は第5 および第6 腰 椎椎間孔より脊柱管内に連続的に波及し，脊髄および脊髄神経根を圧迫していた．腫瘍はCD3 陽性のT細胞性リンパ 腫で，牛白血病ウイルス抗体陰性であったため，散発型牛白血病と診断された． A Holstein cow (5 years and 3 months old) presented with hind limb paresis and was unable to stand. Bovine leukosis was suspected given the enlargement of cervical lymph nodes and existence of an abdominal mass detected by rectal palpation; however，it could not be confirmed by blood examination and fine needle aspiration of cervical lymph nodes. Pathological examination revealed a mu1tinodular mass (60 x 40 x 20 cm) on the dorsal abdomen that was invasive into the iliopsoas muscle. A portion of the... |
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Palavras-chave: 牛白血病; 脊髄浸潤; 後躯麻痺; Bovine leukosis; Spinal cord invasion; Hind limb paresis. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2809 |
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| TAWFEEQ, Mohammad Monir; TAGAWA, Michihito; ITOH, Yuuki; SUGIMOTO, Kazuya; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi; 古林, 与志安; 猪熊, 壽. |
| A 49-month-old Holstein cow with anorexia, tachypnea, enlarged peripheral lymph nodes, and difficulty standing up was suspected of bovine leukosis. Hematological examination revealed lymphocytosis with the presence of neoplastic cells. Increased total lactate dehydrogenase (LDH) activity, isozymes of LDH-2 and LDH-3 activities and thymidine kinase activity were observed. Cytological findings of fine needle aspiration of subiliac lymph nodes indicated lymphosarcoma. Histopathology and antibody analysis confirmed the diagnosis of enzootic bovine leukosis, a B-cell bovine lymphoma caused by bovine leukemia virus. Gene expressions known as biomarkers of hematopoietic neoplasia in human were also examined in the present case. Increased messenger RNA expression... |
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Palavras-chave: Biomarker; Bovine leukosis; Gene expression. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4096 |
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| Gonzalez,E.T.; Oliva,G.A.; Norimine,J.; Cid de la Paz,V.; Echeverría,M.G.. |
| A western blotting (WB) procedure has been developed for detecting antibodies to bovine leukosis virus (BLV) in cattle sera. Two hundred and thirty three serum samples from naturally infected cattle with BLV virus and serial bleedings from experimentally BLV infected cows were used. An agar gel immunodiffusion test (AGID) was used for comparing with the results obtained by WB. The AGID positive sera showed a different degree of reactivity by WB test against the two most important viral antigens (gp51 and p24), or against one of them. Other proteins (gp30, p15, p12 and p10) were not detected with any AGID positive sera, being observed occasionally three bands corresponding to the p24 protein. Using sera obtained by BLV experimental inoculation, the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine leukosis; Western blotting; Diagnosis. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000400002 |
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