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	Provedor de dados BJMBR (1) Genet. Mol. Biol. (1) Autor Costa,Luis Fabiano Santos da (1) Gonçalves,Paulo Bayard Dias (1) Machado,Márcia Silveira Netto (1) Oliveira,João Francisco Coelho de (1) Wang,Z.G. (1) Xu,Z.R. (1) Yu,S.D. (1) Zamberlan,Gustavo (1) Mais... Palavra-chave Bovine oocytes (2) Annexin II (1) Embryo development (1) Green tea polyphenols (1) In vitro culture (1) Retinol (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2007 (1) 2006 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Effect of supplementation of green tea polyphenols on the developmental competence of bovine oocytes in vitro BJMBR Wang,Z.G.; Yu,S.D.; Xu,Z.R.. The objective of the present study was to examine the effect of green tea polyphenols (GTPs) supplementation during in vitro maturation, in vitro fertilization, and in vitro culture on the developmental competence of bovine oocytes. Cumulus-oocyte complexes aspirated from the ovaries were matured in vitro (38.5ºC for 24 h) and fertilized (38.5ºC for 15-18 h) and embryos were cultured (38.5ºC for 192 h) in a defined conditioned medium with or without GTPs supplementation. The GTPs used in the present study contained 99% catechin derivatives, with the major components being 50% (-)-epigallocatechin gallate, 22% (-)-epicatechin gallate, 18% (-)-epigallocatechin, and 10% (-)-epicatechin. Four replicate trials were done for each type of experiment. GTPs... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine oocytes; In vitro culture; Green tea polyphenols; Embryo development. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800008 Annexin II mRNA expression in bovine oocytes during follicular development Genet. Mol. Biol. Costa,Luis Fabiano Santos da; Machado,Márcia Silveira Netto; Oliveira,João Francisco Coelho de; Zamberlan,Gustavo; Gonçalves,Paulo Bayard Dias. We investigated the expression of calcium-dependent phospholipid binding protein annexin-II (Ann-II) messenger RNA (mRNA) during preantral follicle development and in oocytes from antral follicles of different diameters (< 3 mm, 5 to 8 mm and > 8 mm). The action of retinol on Ann-II mRNA expression in mature oocytes was also examined. Only oocytes from secondary preantral follicles expressed Ann-II mRNA and at the germinal vesicle stage expression by oocytes from follicles larger than 8 mm was significantly higher (p < 0.05) compared with oocytes from follicles smaller than 3 mm or between 5 and 8 mm. Ann-II mRNA expression by metaphase II oocytes from follicles larger than 8 mm was significantly higher (p < 0.05) than that from oocytes from... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine oocytes; Annexin II; Retinol. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200031 Registros recuperados: 2 Primeira ... 1 ... Última

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