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Overexpression of the pineapple fruit bromelain gene (BAA) in transgenic Chinese cabbage (Brassica rapa) results in enhanced resistance to bacterial soft rot Electron. J. Biotechnol.
Jung,Yu-Jin; Choi,Chang-Sun; Park,Jin-Heui; Kang,Hee-Wan; Choi,Je-Eul; Nou,Ill-Sup; Lee,Soon Youl; Kang,Kwon-Kyoo.
Bromelain is a crude protein extract obtained from pineapple stems, which comprises a variety of proteolytic enzymes. It exhibits potential therapeutic activities against trauma, inflammation, autoimmune diseases and malignant disorders. In this study, we cloned BAA1 (the gene encoding fruit bromelain) into a plant expression vector that was then used to transform Brassica rapa and overexpress BAA1 under the control of the cauliflower mosaic virus (CaMV) 35S promoter. We demonstrate that constitutive overexpression of BAA1 in B. rapa confers enhanced resistance to the soft rot pathogen Pectobacterium carotovorum ssp. carotovorum. These results suggest that it could be utilized for protecting plants from attack by bacterial pathogens
Tipo: Journal article Palavras-chave: Brassica rapa; Fruit bromelain; Soft rot resistance; Transgenic plant.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100007
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Ammonium/nitrate ratio affects the growth and glucosinolates content of pakchoi Horticultura Brasileira
Yang,Jing; Zhu,Biao; Ni,Xiaolei; He,Yong.
ABSTRACT Nitrogen (N) strongly affects plant growth. However, little is known about the effects of the ammonium/nitrate ratio on pakchoi (Brassica rapa), especially its glucosinolates (GSs) contents which are involved in plant defense and many of them benefit to human health. The aim of this study was to evaluate the effects of a constant N supply (8 mM) but with five ammonium/nitrate ratios (namely 0/8 mM, 2/6 mM, 4/4 mM, 6/2 mM and 8/0 mM) on the growth of pakchoi in a hydroponic system in 2 years. In both years, a higher biomass (dry weight) was in the 4/4 and 2/6 ammonium/nitrate treatments (2.3 and 2.2-fold compared to 8/0, respectively), with no significant difference in biomass between these two treatments. The biomass then decreased with increasing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brassica rapa; Biomass; Nitrogen form; Secondary metabolites.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000300246
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Survey and prevalence of species causing Alternaria leaf spots on brassica species in Pernambuco Horticultura Brasileira
Michereff,Sami J; Noronha,Marissônia A; Xavier Filha,Maria S; Câmara,Marcos PS; Reis,Ailton.
Brassicaceae family comprises plant species that are very important as vegetable crops, such as the species complex Brassica oleracea and Brassica rapa. Alternaria brassicicola and A. brassicae are among the most important pathogens of Brassicaceae causing Alternaria leaf spot disease. The occurrence and prevalence of Alternaria species causing leaf spots in brassica crops in Pernambuco was acessed, as well as the existence of a possible preference by vegetable host for these pathogens. Twenty-eight fields were surveyed in the Agreste region of Pernambuco state, in the 2005 and 2006 growing seasons. In each year, 10 Chinese cabbage, six cabbage, six cauliflower and six broccoli fields were visited. In each field, 50 leaves showing at least five lesions...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brassica oleracea; Brassica rapa; Alternaria brassicae; A. brassicicola; Etiology; Epidemiology.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362012000200027
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Genome-wide Analysis of LBD (LATERAL ORGAN BOUNDARIES Domain) Gene Family in Brassica rapa BABT
Huang,Xiaoyun; Liu,Gang; Zhang,Weiwei.
ABSTRACT LOB (lateral organ boundaries)-domain proteins define a family of plant-specific transcription factors involved in developmental process from embryogenesis to seed production. They play a crucial role in shaping the plant architecture through coordinating cell fate at meristem to organ boundaries. Identification of LBD genes from Brassica rapa genome, and analysis of phylogeny,gene structure, chromosome location, phylogenetic and tissue expression pattern analysis of LBD family genes in Chinese cabbage will be useful to the functions identification of plant LBD genes. Based on Brassica rapa genome database and bioinformatic method, Chinese cabbage LBD family genes were identified and the genes were sequenced. A phylogenetic tree was created using...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brassica rapa; LBD; Phylogenetic analysis; Gene expression; Genome-wide.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100439
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